##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18905937
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.005833088304485	35.0	35.0	35.0	35.0	37.0
2	35.76144334977949	37.0	35.0	37.0	35.0	37.0
3	38.2008680659414	39.0	39.0	39.0	37.0	39.0
4	38.18528597656916	39.0	39.0	39.0	37.0	39.0
5	38.1633497456381	39.0	39.0	39.0	37.0	39.0
6	38.165276336211214	39.0	39.0	39.0	37.0	39.0
7	38.10308846369265	39.0	39.0	39.0	37.0	39.0
8	39.12647725420856	40.0	40.0	40.0	37.0	40.0
9	39.15134346422502	40.0	40.0	40.0	38.0	40.0
10	38.79040372344412	40.0	39.0	40.0	37.0	40.0
11	39.06478896020864	40.0	39.0	40.0	37.0	40.0
12	39.13918019508898	40.0	40.0	40.0	38.0	40.0
13	39.20054589201265	40.0	40.0	40.0	38.0	40.0
14	39.20799445168996	40.0	40.0	40.0	38.0	40.0
15	39.2300532896095	40.0	40.0	40.0	38.0	40.0
16	39.03991735506153	40.0	40.0	40.0	38.0	40.0
17	39.11965987192277	40.0	40.0	40.0	38.0	40.0
18	39.139199289619974	40.0	40.0	40.0	38.0	40.0
19	39.10299579438988	40.0	40.0	40.0	38.0	40.0
20	39.12100251894418	40.0	40.0	40.0	38.0	40.0
21	39.02801437453219	40.0	40.0	40.0	37.0	40.0
22	39.07968184808825	40.0	40.0	40.0	38.0	40.0
23	38.533920059079854	40.0	38.0	40.0	36.0	40.0
24	38.74684465520011	40.0	39.0	40.0	37.0	40.0
25	38.89269386648226	40.0	40.0	40.0	37.0	40.0
26	38.89029435568308	40.0	40.0	40.0	37.0	40.0
27	38.91204947948361	40.0	40.0	40.0	37.0	40.0
28	38.835150566724096	40.0	40.0	40.0	37.0	40.0
29	38.8057148926287	40.0	40.0	40.0	37.0	40.0
30	38.76864997487297	40.0	40.0	40.0	36.0	40.0
31	38.61520087578838	40.0	39.0	40.0	36.0	40.0
32	38.480236763721365	40.0	39.0	40.0	35.0	40.0
33	38.36964864529063	40.0	39.0	40.0	35.0	40.0
34	38.372265601011996	40.0	39.0	40.0	35.0	40.0
35	38.30912723341879	40.0	39.0	40.0	35.0	40.0
36	38.20335585588802	40.0	38.0	40.0	35.0	40.0
37	37.89077399337573	40.0	37.0	40.0	35.0	40.0
38	37.30742448787384	40.0	36.0	40.0	33.0	40.0
39	37.52092720926765	40.0	36.0	40.0	33.0	40.0
40	37.32609899207852	40.0	36.0	40.0	33.0	40.0
41	37.390787930796556	40.0	36.0	40.0	33.0	40.0
42	37.383620182379744	40.0	36.0	40.0	33.0	40.0
43	37.374447666888976	40.0	36.0	40.0	33.0	40.0
44	37.424148192179	40.0	36.0	40.0	33.0	40.0
45	37.38510913264971	40.0	36.0	40.0	33.0	40.0
46	37.36578171185062	40.0	35.0	40.0	33.0	40.0
47	37.12304833132576	40.0	35.0	40.0	33.0	40.0
48	37.24041109414466	40.0	35.0	40.0	33.0	40.0
49	37.18725609844146	40.0	35.0	40.0	33.0	40.0
50	37.0680327031662	40.0	35.0	40.0	33.0	40.0
51	36.76325928728103	39.0	35.0	40.0	33.0	40.0
52	36.52905735378257	39.0	35.0	40.0	32.0	40.0
53	36.07106365582409	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	20.0
13	61.0
14	209.0
15	582.0
16	1249.0
17	2308.0
18	3640.0
19	5232.0
20	7955.0
21	11474.0
22	15855.0
23	21120.0
24	28809.0
25	38173.0
26	49494.0
27	63231.0
28	80345.0
29	99069.0
30	122041.0
31	152782.0
32	196452.0
33	259523.0
34	375132.0
35	598260.0
36	1030749.0
37	2135786.0
38	3576836.0
39	1.0029544E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.162879099829858	52.11172024957028	19.63040498865515	14.094995661944711
2	21.290539580238736	52.33797721847904	12.680857870202361	13.690625331079861
3	47.09339716936537	20.763192006828334	16.187862045663223	15.955548778143077
4	19.12178169217426	19.618398178307693	16.718182230269782	44.541637899248265
5	17.282835545257555	45.96399004185828	19.509220833646065	17.2439535792381
6	43.71021653145253	19.44163888835555	18.473022521972858	18.375122058219066
7	19.148926604378296	19.23116532124274	44.45446951399447	17.165438560384498
8	43.256829851913714	20.388108772392503	19.304840590551002	17.050220785142784
9	43.39659018222689	19.867811894221376	19.48036217406204	17.255235749489696
10	17.626214453163573	20.217024948300633	44.967773879707735	17.18898671882806
11	17.26888225640443	45.74338738143473	19.59866892606275	17.38906143609809
12	17.293498862288605	20.030490951070025	45.12825256954998	17.547757617091392
13	17.290140128997574	20.391959414653716	19.376955503448468	42.940944952900246
14	17.566661731708933	45.94278506270279	19.267370879316907	17.22318232627137
15	17.466127174759972	45.61970665616838	19.35433298016385	17.559833188907803
16	17.910019482239893	19.862279240642767	19.33661367854976	42.89108759856759
17	43.13956510063479	19.821651791180727	19.5397139004536	17.499069207730884
18	17.576505200456342	19.718245120567154	19.53759816294744	43.16765151602907
19	17.624849802472102	45.65443119798823	19.383514289717564	17.337204709822103
20	43.45332897279833	19.989858212264224	19.436196153620948	17.120616661316497
21	17.79310911699325	45.292465536090596	19.559115213385088	17.35531013353107
22	43.19611347483068	19.884727215583126	19.610596396253726	17.308562913332462
23	17.603052416814887	19.918399177993663	45.043469678334375	17.43507872685707
24	17.565635599018446	45.17209594002138	19.501979722031233	17.76028873892894
25	17.54550435664733	19.97901505754515	19.548827439761382	42.926653146046135
26	17.816609671342924	37.19503561235817	27.582208699838574	17.406146016460333
27	18.093739548587305	19.565806233248317	44.896230215936924	17.44422400222745
28	35.58272197775757	19.63519184476284	27.458337558196668	17.323748619282927
29	35.54284032576645	19.70646046265784	27.41665752932531	17.334041682250394
30	35.25099020482296	19.572571303924263	27.532256137318136	17.64418235393464
31	28.28736285326667	22.359156279850083	29.182309239684866	20.17117162719838
32	24.03430731838364	25.274875294464376	30.388856156666556	20.30196123048543
33	23.42199701606961	23.869052351121237	31.167468716308534	21.541481916500622
34	22.527558406652894	23.352108916897375	31.985788379597373	22.13454429685236
35	21.81799294052445	23.75970574745912	32.218826287213375	22.203475024803055
36	21.87071711917796	23.88770257723804	32.31149558998319	21.930084713600813
37	22.954545971458597	23.52540897602695	32.07652178254905	21.443523269965407
38	23.136451792894476	23.721178167471944	31.848064446633877	21.294305592999702
39	23.251764776323967	23.435088141888976	31.83810461232363	21.47504246946343
40	22.67905050143772	24.136761907119443	31.211867467875305	21.97232012356753
41	22.577600888017347	24.8963751439561	31.178116165308285	21.347907802718268
42	22.879590680959108	24.674656431998056	31.30005669647582	21.145696190567016
43	22.770275813359582	24.105850982154443	32.102148653092414	21.021724551393564
44	22.638375447881796	23.849011027594138	31.62419826110708	21.888415263416988
45	22.780230358326065	23.21786008278775	31.628736518057792	22.37317304082839
46	22.65740121740594	23.481110721991723	31.261555563207473	22.599932497394867
47	22.848293633899235	23.624510120815486	31.201087785281416	22.32610846000386
48	22.686027145864283	23.497930835165697	31.37825964404726	22.437782374922755
49	21.926070101682875	24.74261921003968	31.031622500381754	22.299688187895686
50	22.897309982573198	24.194341703349586	30.978866585665653	21.929481728411556
51	22.662854530828067	23.891902316187767	30.973466165681184	22.47177698730298
52	23.140889552313645	23.95253406377055	30.503285819687225	22.40329056422858
53	23.540996672103585	24.27738968981014	29.711418164569153	22.47019547351713
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6887.0
1	4176.0
2	1465.0
3	1190.0
4	915.0
5	929.5
6	944.0
7	1010.5
8	1381.5
9	1686.0
10	2482.0
11	3278.0
12	4833.5
13	6389.0
14	8094.5
15	9800.0
16	13862.0
17	17924.0
18	25182.0
19	32440.0
20	49938.0
21	67436.0
22	648167.5
23	1228899.0
24	775546.0
25	293942.0
26	265691.0
27	270200.5
28	274710.0
29	291496.5
30	308283.0
31	327516.0
32	346749.0
33	383097.5
34	419446.0
35	469072.0
36	518698.0
37	571162.0
38	623626.0
39	684916.5
40	746207.0
41	809312.5
42	937726.0
43	1003034.0
44	1086170.0
45	1169306.0
46	1221298.0
47	1273290.0
48	1360179.5
49	1447069.0
50	1423124.5
51	1399180.0
52	1366783.0
53	1334386.0
54	1295254.0
55	1256122.0
56	1231047.5
57	1205973.0
58	1046526.0
59	759397.0
60	631715.0
61	539862.0
62	448009.0
63	380960.5
64	313912.0
65	256943.5
66	199975.0
67	160672.0
68	121369.0
69	94989.5
70	68610.0
71	50997.5
72	33385.0
73	25221.5
74	17058.0
75	13167.5
76	7000.0
77	4723.0
78	3563.0
79	2403.0
80	1941.5
81	1480.0
82	1187.0
83	894.0
84	595.5
85	297.0
86	204.0
87	111.0
88	80.5
89	50.0
90	43.5
91	37.0
92	26.0
93	9.5
94	4.0
95	4.5
96	5.0
97	5.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8905937E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.77269861470695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.87759312767726	27.868882554899617
2	14.45113548912931	11.206190419206125
3	4.991557661872591	5.806084825251516
4	2.2931606051698745	3.5564810007748213
5	1.3225713248021937	2.563982968650457
6	0.8148593605394785	1.8956577839742015
7	0.5870942961648403	1.5934261142528994
8	0.4455233483318424	1.3819314008548498
9	0.33901124458695303	1.182994273202997
>10	2.525309344514607	20.91658066595307
>50	0.2816980020052467	6.9368032775214665
>100	0.06389290485423124	4.384261450533619
>500	0.0038835301687748618	0.9959250518006508
>1k	0.0023266654225513866	1.8020319199765957
>5k	2.05229335804459E-4	0.5526659797125067
>10k+	1.7786542436386448E-4	7.356100313434519
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1004640	5.313886320471712	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	82625	0.4370320286161961	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	57809	0.30577167373402336	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	44609	0.23595233603073998	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	42280	0.2236334544011228	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	38387	0.2030420391224196	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	29110	0.1539727970108014	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7983768802360867E-4	5.289343765400255E-6	0.0	0.0	5.289343765400255E-6
2	2.1157375061601019E-4	5.289343765400255E-6	0.0	0.0	1.057868753080051E-5
3	2.221524381468107E-4	5.289343765400255E-6	0.0	5.289343765400255E-6	1.5868031296200763E-5
4	2.69756532035413E-4	5.289343765400255E-6	0.0	5.289343765400255E-6	1.5868031296200763E-5
5	2.803352195662135E-4	5.289343765400255E-6	0.0	5.289343765400255E-6	3.1736062592401527E-5
6	2.90913907097014E-4	5.289343765400255E-6	0.0	1.057868753080051E-5	3.1736062592401527E-5
7	2.9620325086241427E-4	5.289343765400255E-6	5.289343765400255E-6	1.057868753080051E-5	3.1736062592401527E-5
8	3.0149259462781454E-4	5.289343765400255E-6	5.289343765400255E-6	1.057868753080051E-5	3.702540635780178E-5
9	3.067819383932148E-4	5.289343765400255E-6	1.057868753080051E-5	1.057868753080051E-5	3.702540635780178E-5
10	3.067819383932148E-4	5.289343765400255E-6	1.057868753080051E-5	1.057868753080051E-5	3.702540635780178E-5
11	3.12071282158615E-4	5.289343765400255E-6	1.057868753080051E-5	2.115737506160102E-5	3.702540635780178E-5
12	3.2793931345481583E-4	5.289343765400255E-6	1.057868753080051E-5	2.6446718827001273E-5	4.231475012320204E-5
13	3.2793931345481583E-4	5.289343765400255E-6	1.057868753080051E-5	2.6446718827001273E-5	4.231475012320204E-5
14	3.3322865722021605E-4	5.289343765400255E-6	1.057868753080051E-5	2.6446718827001273E-5	4.231475012320204E-5
15	3.385180009856163E-4	5.289343765400255E-6	1.057868753080051E-5	3.702540635780178E-5	4.231475012320204E-5
16	3.385180009856163E-4	5.289343765400255E-6	1.057868753080051E-5	3.702540635780178E-5	4.76040938886023E-5
17	3.438073447510166E-4	5.289343765400255E-6	1.057868753080051E-5	3.702540635780178E-5	5.2893437654002547E-5
18	3.543860322818171E-4	5.289343765400255E-6	1.057868753080051E-5	3.702540635780178E-5	5.2893437654002547E-5
19	3.649647198126176E-4	1.5868031296200763E-5	1.057868753080051E-5	3.702540635780178E-5	6.876146895020331E-5
20	3.7025406357801783E-4	1.5868031296200763E-5	1.057868753080051E-5	3.702540635780178E-5	9.52081877772046E-5
21	4.125688137012199E-4	1.5868031296200763E-5	1.057868753080051E-5	3.702540635780178E-5	1.0049753154260485E-4
22	4.2314750123202037E-4	1.5868031296200763E-5	1.057868753080051E-5	3.702540635780178E-5	1.163655628388056E-4
23	4.6017290758982216E-4	1.5868031296200763E-5	1.057868753080051E-5	3.702540635780178E-5	1.163655628388056E-4
24	5.39513064070826E-4	1.5868031296200763E-5	1.057868753080051E-5	4.231475012320204E-5	1.163655628388056E-4
25	5.553810953670267E-4	1.5868031296200763E-5	1.057868753080051E-5	4.76040938886023E-5	1.163655628388056E-4
26	5.976958454902288E-4	2.115737506160102E-5	1.057868753080051E-5	4.76040938886023E-5	1.269442503696061E-4
27	6.347212518480306E-4	2.115737506160102E-5	1.057868753080051E-5	6.347212518480305E-5	1.269442503696061E-4
28	6.770360019712326E-4	2.115737506160102E-5	1.057868753080051E-5	6.347212518480305E-5	1.428122816658069E-4
29	7.034827207982339E-4	2.115737506160102E-5	1.057868753080051E-5	6.347212518480305E-5	1.4810162543120713E-4
30	7.352187833906354E-4	2.115737506160102E-5	1.057868753080051E-5	6.347212518480305E-5	1.5868031296200765E-4
31	7.616655022176367E-4	2.115737506160102E-5	1.057868753080051E-5	6.347212518480305E-5	1.5868031296200765E-4
32	7.934015648100383E-4	2.115737506160102E-5	1.057868753080051E-5	6.347212518480305E-5	1.745483442582084E-4
33	8.3042697116784E-4	2.115737506160102E-5	1.057868753080051E-5	6.347212518480305E-5	1.7983768802360867E-4
34	8.991884401180433E-4	2.115737506160102E-5	1.057868753080051E-5	6.876146895020331E-5	1.7983768802360867E-4
35	9.626605653028464E-4	2.115737506160102E-5	1.057868753080051E-5	6.876146895020331E-5	1.8512703178900892E-4
36	9.891072841298476E-4	2.115737506160102E-5	1.057868753080051E-5	6.876146895020331E-5	1.9570571931980943E-4
37	0.0010208433467222492	2.115737506160102E-5	1.057868753080051E-5	7.405081271560357E-5	1.9570571931980943E-4
38	0.0010737367843762517	2.6446718827001273E-5	1.5868031296200763E-5	7.405081271560357E-5	2.009950630852097E-4
39	0.0011054728469686533	2.6446718827001273E-5	1.5868031296200763E-5	7.405081271560357E-5	2.0628440685060994E-4
40	0.0012059703785112581	3.1736062592401527E-5	1.5868031296200763E-5	7.405081271560357E-5	2.0628440685060994E-4
41	0.0012853105349922619	3.702540635780178E-5	1.5868031296200763E-5	7.934015648100382E-5	2.1157375061601019E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	492805	0.0	46.096825	1
AGCAGTG	500305	0.0	45.422237	2
AGTGGTA	504055	0.0	45.190605	5
GTGGTAT	504115	0.0	45.174038	6
GGTATCA	504070	0.0	45.12166	8
GTATCAA	504110	0.0	45.119476	9
TGGTATC	504800	0.0	45.111343	7
TCAACGC	500540	0.0	45.08963	12
CAACGCA	500735	0.0	45.0716	13
ACGCAGA	501940	0.0	45.07108	15
TATCAAC	501175	0.0	45.044228	10
AACGCAG	501455	0.0	45.028446	14
ATCAACG	502055	0.0	44.982124	11
GCAGTGG	506690	0.0	44.96997	3
AGAGTAC	500600	0.0	44.951843	19
CGCAGAG	503395	0.0	44.931942	16
CAGTGGT	506905	0.0	44.907322	4
CAGAGTA	503560	0.0	44.836956	18
GAGTACT	156560	0.0	44.743965	20
GCAGAGT	508490	0.0	44.63285	17
>>END_MODULE