FastQCFastQC Report
Thu 26 May 2016
SRR805173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15847856
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT969770.611925045255333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT575900.041.7034231
AGCAGTG644200.037.3439142
TCAACGC643500.037.0339612
CAACGCA646450.036.86131713
AGTGGTA656500.036.6979455
ACGCAGA652400.036.63319815
AACGCAG652350.036.5603614
GGTATCA656750.036.5515828
ATCAACG652550.036.5419511
GTATCAA657650.036.4836929
GTGGTAT659650.036.4835136
TGGTATC665900.036.1410877
TATCAAC663050.035.97036710
AGAGTAC662450.035.84330719
CGCAGAG669550.035.6948716
GCAGTGG680100.035.4901473
CAGAGTA683200.034.90603318
CAGTGGT691600.034.8830224
GCAGAGT704400.034.03563317
GAGTACT208950.033.65015420
Produced by FastQC (version 0.11.5)