##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805173_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15847856
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.294082303625174	37.0	37.0	37.0	35.0	37.0
2	36.12682346432224	37.0	37.0	37.0	35.0	37.0
3	38.10306031301647	39.0	39.0	39.0	37.0	39.0
4	38.13346398402408	39.0	39.0	39.0	37.0	39.0
5	38.148842972828625	39.0	39.0	39.0	37.0	39.0
6	38.130054374547576	39.0	39.0	39.0	37.0	39.0
7	38.13194264258837	39.0	39.0	39.0	37.0	39.0
8	39.10954712107429	40.0	40.0	40.0	37.0	40.0
9	39.08230766357291	40.0	40.0	40.0	37.0	40.0
10	39.07295422169409	40.0	40.0	40.0	37.0	40.0
11	39.09902632886114	40.0	40.0	40.0	37.0	40.0
12	39.06519803057272	40.0	40.0	40.0	37.0	40.0
13	39.120588488436546	40.0	40.0	40.0	37.0	40.0
14	39.05372676278734	40.0	40.0	40.0	37.0	40.0
15	39.08414008809772	40.0	40.0	40.0	37.0	40.0
16	38.92775148890803	40.0	40.0	40.0	37.0	40.0
17	39.022132583738774	40.0	40.0	40.0	37.0	40.0
18	39.018348664955056	40.0	40.0	40.0	37.0	40.0
19	38.97334295566542	40.0	40.0	40.0	37.0	40.0
20	38.95589024786697	40.0	40.0	40.0	37.0	40.0
21	38.92466400502377	40.0	40.0	40.0	37.0	40.0
22	38.91319456713892	40.0	40.0	40.0	37.0	40.0
23	38.83658622339829	40.0	39.0	40.0	37.0	40.0
24	38.80146595223985	40.0	40.0	40.0	37.0	40.0
25	38.798897150504146	40.0	40.0	40.0	37.0	40.0
26	38.77117428376432	40.0	40.0	40.0	36.0	40.0
27	38.73460113468977	40.0	40.0	40.0	36.0	40.0
28	38.667962720004525	40.0	39.0	40.0	36.0	40.0
29	38.61508465246025	40.0	39.0	40.0	36.0	40.0
30	38.56525015118764	40.0	39.0	40.0	36.0	40.0
31	38.53083521203121	40.0	39.0	40.0	35.0	40.0
32	38.41774925264339	40.0	39.0	40.0	35.0	40.0
33	38.41138719332129	40.0	39.0	40.0	35.0	40.0
34	38.39142708010471	40.0	39.0	40.0	35.0	40.0
35	38.31643895552812	40.0	39.0	40.0	35.0	40.0
36	38.2584695999257	40.0	38.0	40.0	35.0	40.0
37	38.21913658226072	40.0	38.0	40.0	35.0	40.0
38	37.57062154022601	40.0	38.0	40.0	33.0	40.0
39	37.82935533992737	40.0	38.0	40.0	33.0	40.0
40	37.67073527169858	40.0	38.0	40.0	33.0	40.0
41	37.697037062931415	40.0	38.0	40.0	33.0	40.0
42	37.691768148322396	40.0	38.0	40.0	33.0	40.0
43	37.687658254845324	40.0	38.0	40.0	33.0	40.0
44	37.77869561661842	40.0	38.0	40.0	34.0	40.0
45	37.73353468128433	40.0	38.0	40.0	33.0	40.0
46	37.7330265368388	40.0	38.0	40.0	34.0	40.0
47	37.53899972336952	40.0	37.0	40.0	33.0	40.0
48	37.717468659483025	40.0	37.0	40.0	34.0	40.0
49	37.738586910431295	40.0	37.0	40.0	34.0	40.0
50	37.702558945512884	40.0	37.0	40.0	34.0	40.0
51	37.534891975293064	40.0	37.0	40.0	34.0	40.0
52	37.49333846799214	40.0	37.0	40.0	34.0	40.0
53	37.20088837253443	40.0	36.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	11.0
11	42.0
12	172.0
13	321.0
14	562.0
15	1000.0
16	1697.0
17	2699.0
18	4398.0
19	6333.0
20	9312.0
21	13057.0
22	17519.0
23	23267.0
24	29406.0
25	36577.0
26	45801.0
27	57267.0
28	70287.0
29	87114.0
30	107473.0
31	133506.0
32	166464.0
33	213964.0
34	284592.0
35	413466.0
36	640858.0
37	1162930.0
38	2690247.0
39	9627510.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.692235971856384	28.62273609755162	24.26502991950457	22.41999801108743
2	24.628643773643578	25.872528119892053	26.493716247800332	23.005111858664037
3	25.111119131824523	25.07498806147658	25.26853474690835	24.545358059790548
4	23.34508213603152	26.693484595013988	25.026218057508853	24.935215211445634
5	25.191893464958287	28.82121720439661	24.012137667076228	21.974751663568874
6	26.891460901714403	25.085916984606627	25.649570516036995	22.373051597641975
7	23.513729554332144	25.601090772152396	28.496258421328413	22.388921252187046
8	26.065121995050937	25.263303755410192	25.668904361574207	23.002669887964654
9	25.606277593637905	25.460074851765434	26.08356613033334	22.85008142426332
10	22.48526235977914	25.73876870158336	29.11857603956018	22.657392899077326
11	22.489616261026097	28.643596963526168	26.01670535118441	22.85008142426332
12	22.462565283278696	25.541858785188353	28.959305283945035	23.036270647587916
13	22.567929693455064	25.7564619466507	25.744043863094163	25.931564496800068
14	22.904265409781612	28.61110045421917	25.683347955710857	22.801286180288365
15	22.83755607067606	28.335290275227138	25.702038181063735	23.125115473033073
16	23.1956676032392	25.404496355847755	25.477250676684594	25.92258536422845
17	25.954659103414368	25.416945989413332	25.67753013404463	22.950864773127673
18	22.859710487021083	25.39901296427731	25.71066395353416	26.030612595167447
19	23.121007661856595	28.52667894004085	25.537820384031757	22.8144930140708
20	26.07112911677138	25.339250937161466	25.7252779177196	22.864342028347558
21	22.97505101005461	28.246420209774747	25.78203007397341	22.99649870619723
22	26.012641710020585	25.310830689021905	25.73086857932076	22.945659021636743
23	22.938774809665105	25.383591319860553	28.65296731620984	23.0246665542645
24	22.858467416665068	28.32653830272057	25.609091854443903	23.205902426170454
25	23.088795102630918	25.307063617943022	25.64352553430571	25.960615745120347
26	23.27257390526517	27.298506498292262	26.597856517626106	22.831063078816467
27	23.32070659905037	25.075467621613928	28.61705709592515	22.986768683410553
28	25.58534731764347	25.189792234356496	26.36884762203796	22.856012825962075
29	25.42791277255422	25.196909916395	26.43209277015137	22.943084540899413
30	25.211631150611165	25.157630155145277	26.505793591259284	23.124945102984277
31	24.47630140001272	25.635896742120828	26.596272707172503	23.29152915069395
32	23.900646245145087	25.933823477447042	26.764068275229153	23.401462002178715
33	23.676754761022565	25.687411596874682	26.934886334151447	23.70094730795131
34	23.706468559532595	25.758531627243457	26.962827022153657	23.572172791070287
35	23.676578080971964	25.708587962939593	26.968771043856027	23.64606291223242
36	23.582294033969013	25.718942675905183	26.980299417157756	23.718463872968055
37	23.80908180892103	25.7832479043222	26.843738358046664	23.563931928710105
38	23.87322297729106	25.87719121122756	26.700375116987434	23.549210694493944
39	23.798872225997005	25.66240505971281	26.882847749247595	23.65587496504259
40	24.185706886786452	25.72883675873885	26.601200818583916	23.484255535890785
41	23.75299220285697	25.859087816042752	26.831711494602175	23.556208486498107
42	23.7676881970659	25.74815167427064	26.832973494963607	23.651186633699854
43	23.916238259610637	25.738894901619503	26.762881994889405	23.58198484388046
44	23.837155006961193	25.71607793508472	26.758376653599075	23.68839040435501
45	23.739482488987782	25.57752291540256	26.837756476333457	23.845238119276196
46	23.882517609953045	25.714670804681717	26.669714818206323	23.733096767158916
47	24.033011153054396	25.706234332265517	26.656672044470874	23.60408247020922
48	23.800828326557234	25.643594944325592	26.771192267269466	23.78438446184771
49	23.813915270305333	25.854752844801215	26.64022817976135	23.691103705132104
50	23.854223561849626	25.797830318498605	26.705681828507277	23.642264291144492
51	23.88850580166806	25.64507148474847	26.64157852014809	23.824844193435375
52	23.892468482802972	25.794757347618503	26.548758393564402	23.76401577601412
53	23.89238645277948	25.831020928004396	26.500284959681615	23.776307659534513
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4668.0
1	3083.5
2	1499.0
3	1357.0
4	1215.0
5	1268.0
6	1321.0
7	1430.0
8	1849.0
9	2159.0
10	2871.0
11	3583.0
12	5203.5
13	6824.0
14	8743.5
15	10663.0
16	15265.0
17	19867.0
18	27395.5
19	34924.0
20	47065.0
21	59206.0
22	134061.0
23	208916.0
24	177281.5
25	162936.5
26	180226.0
27	204460.0
28	228694.0
29	257626.0
30	286558.0
31	313903.0
32	341248.0
33	380335.5
34	419423.0
35	469164.5
36	518906.0
37	571580.5
38	624255.0
39	673403.0
40	722551.0
41	780377.5
42	893127.0
43	948050.0
44	999358.5
45	1050667.0
46	1086809.5
47	1122952.0
48	1145638.5
49	1168325.0
50	1167960.5
51	1167596.0
52	1145272.0
53	1122948.0
54	1087722.0
55	1052496.0
56	987961.5
57	923427.0
58	843669.5
59	679526.5
60	595141.0
61	522574.0
62	450007.0
63	387131.5
64	324256.0
65	268686.5
66	213117.0
67	172323.5
68	131530.0
69	103977.5
70	76425.0
71	56596.0
72	36767.0
73	27667.0
74	18567.0
75	14482.5
76	7651.5
77	4905.0
78	3521.5
79	2138.0
80	1658.5
81	1179.0
82	871.0
83	563.0
84	372.0
85	181.0
86	127.5
87	74.0
88	73.0
89	72.0
90	51.5
91	31.0
92	27.0
93	14.5
94	6.0
95	5.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.5847856E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.3141857509435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.60774403467357	35.80591776282853
2	14.05810877070652	13.865283744511785
3	4.984428574702594	7.37409109785583
4	2.3249945435831534	4.586208511687589
5	1.375661908900288	3.391982345300316
6	0.8671479727731555	2.565761772173369
7	0.6175323164910672	2.1317172353855645
8	0.47296457812218756	1.8659090447307347
9	0.370953409703802	1.6463938815971226
>10	2.1857356359718576	19.808106902965243
>50	0.10585363281613577	3.507809912220617
>100	0.02798920598558668	2.323463568965027
>500	6.414956447423532E-4	0.19564307033177775
>1k	2.0541062299211856E-4	0.23648614527470255
>5k	2.5672868225011696E-5	0.08134285106219612
>10k+	1.2836434112505848E-5	0.6138821531096181
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96977	0.611925045255333	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3347006686582714E-4	6.310001807184518E-6	0.0	1.2620003614369035E-5	6.310001807184518E-6
2	2.524000722873807E-4	1.2620003614369035E-5	0.0	1.2620003614369035E-5	6.310001807184518E-6
3	2.7133007770893425E-4	1.2620003614369035E-5	0.0	1.2620003614369035E-5	6.310001807184518E-6
4	2.7133007770893425E-4	1.2620003614369035E-5	0.0	1.2620003614369035E-5	1.8930005421553553E-5
5	2.839500813233033E-4	1.8930005421553553E-5	0.0	1.2620003614369035E-5	3.1550009035922585E-5
6	2.839500813233033E-4	1.8930005421553553E-5	0.0	1.2620003614369035E-5	3.7860010843107106E-5
7	2.965700849376723E-4	1.8930005421553553E-5	0.0	1.2620003614369035E-5	3.7860010843107106E-5
8	3.0919008855204134E-4	1.8930005421553553E-5	0.0	1.2620003614369035E-5	4.417001265029162E-5
9	3.281200939735949E-4	2.524000722873807E-5	0.0	1.8930005421553553E-5	5.048001445747614E-5
10	3.5336010120233296E-4	2.524000722873807E-5	0.0	1.8930005421553553E-5	7.572002168621421E-5
11	3.6598010481670205E-4	3.1550009035922585E-5	0.0	1.8930005421553553E-5	7.572002168621421E-5
12	3.6598010481670205E-4	3.1550009035922585E-5	0.0	2.524000722873807E-5	7.572002168621421E-5
13	3.7229010662388653E-4	3.1550009035922585E-5	0.0	3.1550009035922585E-5	8.203002349339873E-5
14	3.8491011023825556E-4	5.048001445747614E-5	0.0	3.1550009035922585E-5	1.072700307221368E-4
15	3.975301138526246E-4	5.048001445747614E-5	0.0	3.7860010843107106E-5	1.2620003614369034E-4
16	4.101501174669936E-4	5.6790016264660656E-5	0.0	4.417001265029162E-5	1.6406004698679745E-4
17	4.4801012831010076E-4	5.6790016264660656E-5	0.0	4.417001265029162E-5	1.8299005240835102E-4
18	4.732501355388388E-4	5.6790016264660656E-5	0.0	5.048001445747614E-5	1.8930005421553554E-4
19	4.8587013915320785E-4	5.6790016264660656E-5	0.0	5.048001445747614E-5	2.0192005782990457E-4
20	5.048001445747614E-4	5.6790016264660656E-5	0.0	6.310001807184517E-5	2.145400614442736E-4
21	5.174201481891304E-4	6.310001807184517E-5	0.0	6.94100198790297E-5	2.208500632514581E-4
22	5.36350153610684E-4	6.310001807184517E-5	0.0	6.94100198790297E-5	2.2716006505864262E-4
23	5.679001626466066E-4	6.310001807184517E-5	0.0	6.94100198790297E-5	2.3347006686582714E-4
24	5.805201662609756E-4	6.310001807184517E-5	0.0	6.94100198790297E-5	2.3347006686582714E-4
25	6.120701752968982E-4	6.310001807184517E-5	0.0	6.94100198790297E-5	2.460900704801962E-4
26	6.183801771040827E-4	6.94100198790297E-5	0.0	6.94100198790297E-5	2.524000722873807E-4
27	6.436201843328208E-4	6.94100198790297E-5	0.0	8.203002349339873E-5	2.587100740945652E-4
28	6.625501897543744E-4	7.572002168621421E-5	0.0	8.203002349339873E-5	2.587100740945652E-4
29	7.256502078262196E-4	8.203002349339873E-5	0.0	8.203002349339873E-5	2.776400795161188E-4
30	8.076802313196183E-4	8.834002530058324E-5	0.0	8.203002349339873E-5	2.965700849376723E-4
31	8.203002349339872E-4	8.834002530058324E-5	0.0	8.203002349339873E-5	3.0919008855204134E-4
32	8.770902511986479E-4	8.834002530058324E-5	0.0	8.834002530058324E-5	3.218100921664104E-4
33	9.528102728848622E-4	8.834002530058324E-5	0.0	8.834002530058324E-5	3.4074009758796393E-4
34	0.0010222202927638918	8.834002530058324E-5	0.0	8.834002530058324E-5	3.5336010120233296E-4
35	0.001072700307221368	8.834002530058324E-5	0.0	8.834002530058324E-5	3.8491011023825556E-4
36	0.0011105603180644752	9.465002710776777E-5	0.0	8.834002530058324E-5	4.1646011927417816E-4
37	0.0011673503343291357	9.465002710776777E-5	0.0	9.465002710776777E-5	4.227701210813627E-4
38	0.001280930366858457	9.465002710776777E-5	0.0	9.465002710776777E-5	4.3539012469573173E-4
39	0.0013503403867374867	9.465002710776777E-5	0.0	9.465002710776777E-5	4.4801012831010076E-4
40	0.0015144004337242843	9.465002710776777E-5	0.0	9.465002710776777E-5	4.4801012831010076E-4
41	0.0017163204915541888	9.465002710776777E-5	0.0	9.465002710776777E-5	4.669401337316543E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	57590	0.0	41.703423	1
AGCAGTG	64420	0.0	37.343914	2
TCAACGC	64350	0.0	37.03396	12
CAACGCA	64645	0.0	36.861317	13
AGTGGTA	65650	0.0	36.697945	5
ACGCAGA	65240	0.0	36.633198	15
AACGCAG	65235	0.0	36.56036	14
GGTATCA	65675	0.0	36.551582	8
ATCAACG	65255	0.0	36.54195	11
GTATCAA	65765	0.0	36.483692	9
GTGGTAT	65965	0.0	36.483513	6
TGGTATC	66590	0.0	36.141087	7
TATCAAC	66305	0.0	35.970367	10
AGAGTAC	66245	0.0	35.843307	19
CGCAGAG	66955	0.0	35.69487	16
GCAGTGG	68010	0.0	35.490147	3
CAGAGTA	68320	0.0	34.906033	18
CAGTGGT	69160	0.0	34.883022	4
GCAGAGT	70440	0.0	34.035633	17
GAGTACT	20895	0.0	33.650154	20
>>END_MODULE