FastQCFastQC Report
Thu 26 May 2016
SRR1041764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25186829
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1690730.6712754511494877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1486470.5901775090464941No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1235760.4906373882952872No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500610.198758644845685No Hit
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC307420.12205585705131836No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA288600.11458369769374303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA782100.019.4319761
TAGCGCA69850.014.566934
GTATCAA1316350.014.4686071
TAGTCCC106300.013.679215
CTACTCG103850.013.57438614
ATAGCGC76700.013.2418513
TACTCGG106650.013.11392415
TGAACCG87100.012.99885214
TAAACGC41450.012.72014528
AGCGCAC79900.012.7115145
TATACAG58800.012.5221085
AACCGGC90300.012.497231516
ACGCTAC26700.012.40262227
GCCTAAG103650.012.3868791
ACCGATT30150.012.33333220
TCGGAAA90300.012.31284628
GAACCGG92850.012.29348415
TTAGGCA120200.012.2358564
CGGAAAC91100.012.22502729
ACGACCG31500.012.15714317
Produced by FastQC (version 0.11.5)