##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041764_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25186829
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51200554067366	34.0	31.0	34.0	31.0	34.0
2	32.66558481816032	34.0	31.0	34.0	31.0	34.0
3	32.695006902218616	34.0	31.0	34.0	31.0	34.0
4	36.15901485653474	37.0	35.0	37.0	35.0	37.0
5	36.123437015433744	37.0	35.0	37.0	35.0	37.0
6	36.19335649596859	37.0	37.0	37.0	35.0	37.0
7	36.10881016423306	37.0	36.0	37.0	35.0	37.0
8	36.13009958498547	37.0	36.0	37.0	35.0	37.0
9	37.89562211265261	39.0	38.0	39.0	35.0	39.0
10	37.82212782720683	39.0	38.0	39.0	35.0	39.0
11	37.90217803916484	39.0	38.0	39.0	35.0	39.0
12	37.82301980134141	39.0	38.0	39.0	35.0	39.0
13	37.82701319804887	39.0	38.0	39.0	35.0	39.0
14	38.67486105535556	40.0	39.0	40.0	36.0	40.0
15	38.64627091405591	40.0	39.0	40.0	36.0	40.0
16	38.62722333962723	40.0	39.0	40.0	36.0	40.0
17	38.639236721700854	40.0	39.0	40.0	36.0	40.0
18	38.615089061032656	40.0	39.0	40.0	36.0	40.0
19	38.61316960543147	40.0	39.0	40.0	36.0	40.0
20	38.55163395122109	40.0	39.0	40.0	35.0	40.0
21	38.469842472031715	40.0	38.0	40.0	35.0	40.0
22	38.42209751771452	40.0	38.0	40.0	35.0	40.0
23	38.33063272871706	40.0	38.0	40.0	35.0	40.0
24	38.278337261113734	40.0	38.0	40.0	34.0	40.0
25	38.249723059619775	40.0	38.0	40.0	34.0	40.0
26	38.121486472155745	40.0	38.0	40.0	34.0	40.0
27	38.02312283932209	40.0	38.0	40.0	34.0	40.0
28	37.90953033428702	40.0	38.0	40.0	34.0	40.0
29	37.80672894551355	40.0	38.0	40.0	33.0	40.0
30	37.805477180156345	40.0	38.0	40.0	33.0	40.0
31	37.715896510831115	40.0	37.0	40.0	33.0	40.0
32	37.637607814782875	40.0	37.0	40.0	33.0	40.0
33	37.550164294203135	40.0	37.0	40.0	33.0	40.0
34	37.445116493227474	40.0	37.0	40.0	33.0	40.0
35	37.349475235647965	40.0	37.0	40.0	33.0	40.0
36	37.41225205443686	40.0	37.0	40.0	33.0	40.0
37	37.377357824599514	40.0	37.0	40.0	33.0	40.0
38	37.308900695677096	40.0	37.0	40.0	33.0	40.0
39	37.23597746266511	40.0	37.0	40.0	33.0	40.0
40	37.16607044896362	40.0	37.0	40.0	33.0	40.0
41	37.05503690043713	40.0	36.0	40.0	32.0	40.0
42	36.95281363128324	40.0	36.0	40.0	32.0	40.0
43	36.58955051467574	40.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	9.0
11	14.0
12	42.0
13	45.0
14	83.0
15	102.0
16	214.0
17	386.0
18	777.0
19	1505.0
20	3233.0
21	6661.0
22	12650.0
23	23063.0
24	38491.0
25	61035.0
26	91494.0
27	133248.0
28	189327.0
29	257419.0
30	337835.0
31	428074.0
32	526118.0
33	664490.0
34	868381.0
35	1165541.0
36	1730214.0
37	3165838.0
38	1.0292064E7
39	5188475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.536629482020146	17.835512362433555	12.07754259180463	24.550315563741666
2	18.849169143126353	21.55892272107775	33.99978615807492	25.59212197772098
3	19.97777092146058	23.53412571308599	29.92445376907113	26.563649596382298
4	14.109549082181008	15.862838470059092	35.002953329297625	35.02465911846227
5	14.56434233940287	35.78799062001811	33.810282350350654	15.837384690228372
6	33.42694310585902	35.366710910690664	14.74747376892899	16.458872214521328
7	29.359452116818673	31.028622142152155	20.624815454140734	18.987110286888438
8	28.526425458321885	32.59175261800522	18.74301445410218	20.1388074695707
9	26.666540674890037	14.10242631178383	19.589889620483785	39.64114339284235
10	17.413113020301203	27.60071146709258	30.61949163985669	24.366683872749523
11	35.919674525125814	22.3490976176477	20.611745130758617	21.11948272646787
12	23.53361751096178	25.777099610276466	28.113677986220498	22.575604892541257
13	31.186502278631423	19.96307276314934	24.28064684125183	24.569778116967402
14	22.617305259030424	20.585445670830577	26.215157930361144	30.582091139777855
15	25.147310127844996	27.502556991195675	23.53213657820919	23.81799630275014
16	24.259155449858337	27.119837118042927	24.106162788495528	24.514844643603208
17	23.642333062252497	26.336943805033975	25.057683918845044	24.963039213868488
18	25.00263927626618	24.17486139283353	26.663936139003447	24.158563191896846
19	26.475413002565745	24.22238226177658	26.310870653864367	22.991334081793305
20	25.776099087344424	24.444438797754174	26.884333077419154	22.89512903748225
21	24.92710773555496	25.216457379370784	26.416862559395625	23.439572325678633
22	24.53236173557219	24.841999761065594	26.388534261299824	24.237104242062387
23	24.003037460571157	25.11899771106557	26.914551252164376	23.963413576198896
24	23.772841749947958	25.14810816399317	26.93298946048349	24.146060625575373
25	24.315593677949693	24.798155416864905	27.133042432614285	23.75320847257112
26	23.74101162159	25.346799313244233	27.118943793996458	23.793245271169308
27	24.053877524637976	24.889746938767082	27.205326244125448	23.85104929246949
28	23.67179687446959	25.19853531383407	27.017017505458906	24.112650306237438
29	23.53289094073732	25.471678074282394	26.721783833923674	24.27364715105661
30	23.6125278017332	25.51361666051729	26.63459540698831	24.2392601307612
31	23.475535566624924	25.412234307065805	26.867518733700063	24.244711392609208
32	23.197795165084102	25.846401704636975	26.643020445328787	24.312782684950136
33	23.24018636883587	25.893819345023545	27.03949353846806	23.826500747672526
34	23.72842964868662	25.488071563117376	26.960936606986134	23.822562181209868
35	23.338408340327398	25.982377535496827	27.11811796554461	23.561096158631166
36	23.078578093336006	25.388432184138782	27.211658919032644	24.32133080349257
37	23.67003801868032	25.051402858216093	26.870536183812575	24.40802293929101
38	23.170606351438682	25.053646094155003	27.533354833988827	24.242392720417484
39	23.218949078504483	24.883620720972853	27.472060893413776	24.425369307108884
40	23.144255277232396	24.775290291604392	27.644583603596946	24.435870827566262
41	22.60585482991924	24.083178553362156	28.310574546720428	25.000392069998174
42	22.47265822942618	23.670581953766394	28.65294793560555	25.203811881201876
43	22.099614048278966	23.18205281022077	29.02078304497958	25.69755009652069
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2423.0
1	2490.5
2	2558.0
3	9942.0
4	17326.0
5	17326.0
6	26377.5
7	35429.0
8	34368.5
9	33308.0
10	44703.0
11	56098.0
12	56098.0
13	97680.5
14	139263.0
15	226156.0
16	313049.0
17	316034.0
18	319019.0
19	319019.0
20	295942.0
21	272865.0
22	226930.5
23	180996.0
24	223403.5
25	265811.0
26	265811.0
27	330303.5
28	394796.0
29	484654.0
30	574512.0
31	654858.0
32	735204.0
33	735204.0
34	820727.0
35	906250.0
36	987064.5
37	1067879.0
38	1135396.5
39	1202914.0
40	1202914.0
41	1248446.5
42	1293979.0
43	1355387.0
44	1416795.0
45	1490761.5
46	1564728.0
47	1564728.0
48	1653322.5
49	1741917.0
50	1767686.0
51	1793455.0
52	1832924.5
53	1872394.0
54	1872394.0
55	1817194.0
56	1761994.0
57	1711429.0
58	1660864.0
59	1574865.0
60	1488866.0
61	1488866.0
62	1332096.0
63	1175326.0
64	1011149.5
65	846973.0
66	751319.0
67	655665.0
68	655665.0
69	561705.0
70	467745.0
71	406813.0
72	345881.0
73	279654.5
74	213428.0
75	213428.0
76	173771.0
77	134114.0
78	116394.5
79	98675.0
80	80226.0
81	61777.0
82	61777.0
83	49391.5
84	37006.0
85	30144.0
86	23282.0
87	16248.0
88	9214.0
89	9214.0
90	5696.5
91	2179.0
92	1380.5
93	582.0
94	412.5
95	243.0
96	243.0
97	136.0
98	29.0
99	23.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2.5186829E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.11714075822371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92503888906035	28.52619011022312
2	13.542297285426905	10.594718982075104
3	5.069166634093244	5.948739142581496
4	2.4150232212222114	3.7787521311571233
5	1.4292888224132276	2.7954846025247018
6	0.8708728748002339	2.0439634095647814
7	0.5985289552212534	1.6388918972483641
8	0.43823024453275616	1.3713851327918896
9	0.32498476134720056	1.1441227188506566
>10	2.073606810503289	15.387235973494912
>50	0.17968704468700344	4.859938626303724
>100	0.11548880214896566	8.654521539565255
>500	0.009822397081532241	2.6512364764508844
>1k	0.007041421946487461	5.491477183932783
>5k	6.685840001976528E-4	1.6895968910403187
>10k+	2.5325151522521467E-4	3.423745182194988
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	169073	0.6712754511494877	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	148647	0.5901775090464941	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	123576	0.4906373882952872	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50061	0.198758644845685	No Hit
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC	30742	0.12205585705131836	No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA	28860	0.11458369769374303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1910987286251874E-5	0.0	0.0	2.779230366792104E-5	1.5881316381669167E-5
2	4.4467685868673665E-4	0.0	0.0	2.6204172029754125E-4	1.9851645477086457E-5
3	6.074603515988456E-4	0.0	0.0	3.4938896039672163E-4	1.9851645477086457E-5
4	6.233416679805148E-4	0.0	0.0	3.6129994768297353E-4	2.3821974572503747E-5
5	0.002016927180471984	0.0	0.0	0.0010838998430489205	2.779230366792104E-5
6	0.0027911413540783556	0.0	0.0	0.0013697635379189654	5.1614278240424784E-5
7	0.003084945707139235	0.0	0.0	0.0014650514362089806	6.352526552667667E-5
8	0.003926655475367701	0.0	0.0	0.0018382623711782058	6.749559462209396E-5
9	0.004172815879283573	0.0	0.0	0.0019851645477086456	1.0322855648084957E-4
10	0.007067185789842778	0.0	0.0	0.0029380435306087956	1.0322855648084957E-4
11	0.007706408774204963	0.0	0.0	0.0031802336054292503	1.0719888557626687E-4
12	0.008067708721887936	0.0	0.0	0.0032914028201009343	1.389615183396052E-4
13	0.008687080060773034	0.0	0.0	0.0034978599330626335	1.6675382200752624E-4
14	0.010036991953214912	0.0	0.0	0.003827397247982269	1.9851645477086456E-4
15	0.010958108303351723	3.970329095417292E-6	3.970329095417292E-6	0.0040537060064210545	2.0248678386628185E-4
16	0.011517924705805562	3.970329095417292E-6	3.970329095417292E-6	0.005177309140424148	2.1042744205711643E-4
17	0.011756144451530599	7.940658190834583E-6	3.970329095417292E-6	0.006622508931156042	2.1042744205711643E-4
18	0.01191495761534729	7.940658190834583E-6	3.970329095417292E-6	0.007940658190834582	2.3821974572503748E-4
19	0.012331842170366107	7.940658190834583E-6	3.970329095417292E-6	0.009695543651009025	2.4616040391587204E-4
20	0.014924467069673599	7.940658190834583E-6	3.970329095417292E-6	0.016290260278497146	2.6204172029754125E-4
21	0.02273410440035941	7.940658190834583E-6	3.970329095417292E-6	0.03373985665285614	2.739527075837931E-4
22	0.023067612044374464	7.940658190834583E-6	3.970329095417292E-6	0.048227587522033835	2.739527075837931E-4
23	0.023341564751958255	7.940658190834583E-6	3.970329095417292E-6	0.05619603801653634	3.2953731491963515E-4
24	0.02376241963607249	7.940658190834583E-6	3.970329095417292E-6	0.06630846622256419	3.3747797311046974E-4
25	0.024139600900137132	7.940658190834583E-6	3.970329095417292E-6	0.07156518194489668	3.4144830220588705E-4
26	0.026613115926582102	7.940658190834583E-6	3.970329095417292E-6	0.0815386486325849	3.4938896039672163E-4
27	0.027272190556421372	7.940658190834583E-6	3.970329095417292E-6	0.12563312356628936	3.4938896039672163E-4
28	0.027855828933447718	7.940658190834583E-6	3.970329095417292E-6	0.22842097351754762	3.4938896039672163E-4
29	0.02795508716083315	7.940658190834583E-6	3.970329095417292E-6	0.4025079933643096	3.5335928949213895E-4
30	0.028078167362791083	7.940658190834583E-6	3.970329095417292E-6	0.5827291716634913	3.5335928949213895E-4
31	0.02843946731047406	7.940658190834583E-6	3.970329095417292E-6	0.6863785830284551	3.573296185875562E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	78210	0.0	19.431976	1
TAGCGCA	6985	0.0	14.56693	4
GTATCAA	131635	0.0	14.468607	1
TAGTCCC	10630	0.0	13.67921	5
CTACTCG	10385	0.0	13.574386	14
ATAGCGC	7670	0.0	13.241851	3
TACTCGG	10665	0.0	13.113924	15
TGAACCG	8710	0.0	12.998852	14
TAAACGC	4145	0.0	12.720145	28
AGCGCAC	7990	0.0	12.711514	5
TATACAG	5880	0.0	12.522108	5
AACCGGC	9030	0.0	12.4972315	16
ACGCTAC	2670	0.0	12.402622	27
GCCTAAG	10365	0.0	12.386879	1
ACCGATT	3015	0.0	12.333332	20
TCGGAAA	9030	0.0	12.312846	28
GAACCGG	9285	0.0	12.293484	15
TTAGGCA	12020	0.0	12.235856	4
CGGAAAC	9110	0.0	12.225027	29
ACGACCG	3150	0.0	12.157143	17
>>END_MODULE