FastQCFastQC Report
Thu 26 May 2016
SRR1041763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25737091
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT434540.1688380400100384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT366770.14250639281649974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT284460.11052531150470735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA194100.025.2957231
GTATCAA398950.018.057151
CTACTCG69600.012.83836114
AACGCAG555850.012.7504726
TATCAAC561000.012.7092682
ATCAACG562300.012.5877633
TCAACGC563350.012.5840054
CAACGCA568200.012.46682555
TAGTCCC76150.012.36572555
TACTCGG72000.012.28194315
ACGCAGA585750.012.0680317
CGCAGAG601000.011.7648918
ATCTCCG79850.011.74639910
ACGAGAC78000.011.64551322
TAGCGCA49400.011.4595144
AGAGTAC615500.011.45767711
CGTATAC6350.011.3622043
CTGTCTC1196750.011.22753237
AGACCGA81100.011.22318125
CCCACGA82450.011.19648219
Produced by FastQC (version 0.11.5)