##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041763_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25737091
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1470564019842	33.0	31.0	34.0	30.0	34.0
2	32.243483966389206	34.0	31.0	34.0	30.0	34.0
3	32.36421241235072	34.0	31.0	34.0	30.0	34.0
4	35.918565893868895	37.0	35.0	37.0	35.0	37.0
5	35.86960674770898	37.0	35.0	37.0	35.0	37.0
6	35.928150426946075	37.0	35.0	37.0	35.0	37.0
7	35.865253108830366	37.0	35.0	37.0	35.0	37.0
8	35.85417194196501	37.0	35.0	37.0	35.0	37.0
9	37.57830572227452	39.0	37.0	39.0	35.0	39.0
10	37.46679580065983	39.0	37.0	39.0	34.0	39.0
11	37.552555609334405	39.0	37.0	39.0	35.0	39.0
12	37.46554946710955	39.0	37.0	39.0	34.0	39.0
13	37.4836507746738	39.0	37.0	39.0	34.0	39.0
14	38.34147802484748	40.0	38.0	40.0	34.0	40.0
15	38.33028829870477	40.0	38.0	40.0	34.0	40.0
16	38.29956349767734	40.0	38.0	40.0	34.0	40.0
17	38.274190194999115	40.0	38.0	40.0	34.0	40.0
18	38.22416717569208	40.0	38.0	40.0	34.0	40.0
19	38.23033729802641	40.0	38.0	40.0	34.0	40.0
20	38.16763094943403	40.0	38.0	40.0	34.0	40.0
21	38.11439591210988	40.0	38.0	40.0	34.0	40.0
22	38.06926081894803	40.0	38.0	40.0	34.0	40.0
23	37.99860407689432	40.0	38.0	40.0	34.0	40.0
24	37.93696268937309	40.0	38.0	40.0	33.0	40.0
25	37.90916055742275	40.0	38.0	40.0	33.0	40.0
26	37.774533493315154	40.0	38.0	40.0	33.0	40.0
27	37.70009602095279	40.0	38.0	40.0	33.0	40.0
28	37.56265702289353	40.0	37.0	40.0	33.0	40.0
29	37.50259394117229	40.0	37.0	40.0	32.0	40.0
30	37.413442956704	40.0	37.0	40.0	32.0	40.0
31	37.354792427784474	40.0	37.0	40.0	32.0	40.0
32	37.224823232742196	40.0	37.0	40.0	32.0	40.0
33	37.3165692268796	40.0	37.0	40.0	32.0	40.0
34	37.24622872103145	40.0	37.0	40.0	32.0	40.0
35	37.397625240552635	40.0	37.0	40.0	32.0	40.0
36	37.44605180904089	40.0	37.0	40.0	33.0	40.0
37	37.421837534008795	40.0	37.0	40.0	32.0	40.0
38	37.38562594350698	40.0	37.0	40.0	32.0	40.0
39	37.35712272222218	40.0	37.0	40.0	32.0	40.0
40	37.33902965179709	40.0	37.0	40.0	32.0	40.0
41	37.31645577194408	40.0	37.0	40.0	32.0	40.0
42	37.28921932163973	40.0	37.0	40.0	32.0	40.0
43	36.98013326370101	40.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	13.0
13	18.0
14	19.0
15	34.0
16	87.0
17	156.0
18	465.0
19	1112.0
20	2691.0
21	5921.0
22	12358.0
23	23749.0
24	41276.0
25	68372.0
26	104706.0
27	155363.0
28	220390.0
29	304125.0
30	401973.0
31	518845.0
32	652099.0
33	821628.0
34	1056074.0
35	1389041.0
36	1957404.0
37	3238181.0
38	1.0479713E7
39	4281270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.965838680059065	19.180905099181565	12.129968379099253	25.723287841660113
2	17.889982205059614	22.07227693292921	34.66627599832475	25.37146486368642
3	19.162647402536674	23.791433927012186	30.611971648233283	26.433947022217858
4	14.522476530078709	16.393496063715983	35.75042727245282	33.33360013375249
5	14.294801226758688	36.13819448359568	34.99063277974967	14.576371509895973
6	33.12648270933183	36.33122329170767	15.928462933126358	14.613831065834129
7	28.581466335880773	31.314269355460567	21.615496483266117	18.488767825392543
8	26.85931366524678	34.160457372591175	20.302982182407483	18.677246779754558
9	26.0318464118575	15.15058947415619	19.919119841477034	38.89844427250927
10	16.28084930033468	27.781717832835113	32.88606315298026	23.051369713849944
11	35.60682518471104	22.432671198155223	21.97810545100066	19.982398166133073
12	22.19436532279425	25.098057896286726	29.764175757081485	22.94340102383754
13	29.749197374326414	20.44219371956217	25.003758194739255	24.804850711372158
14	21.919586794016467	20.422191459011433	26.287629009820883	31.37059273715122
15	24.231133969258607	28.26346225375665	23.187538949137647	24.317864827847092
16	24.27389715488825	27.456887027364512	24.439246067086604	23.82996975066063
17	23.24257624919615	27.1664734759651	25.895615009481844	23.695335265356913
18	24.064308588721232	26.152936242872205	26.66593516726502	23.116820001141544
19	25.02221016353402	25.88018591533907	26.229001560432764	22.868602360694144
20	24.609210885565894	25.966030892924145	26.327167277762666	23.097590943747296
21	24.40494537630535	26.146202770157668	26.042947122501143	23.405904731035843
22	24.426412448866113	25.807399911668337	25.986884065491317	23.779303573974232
23	24.080623563867416	25.862526576915783	26.092482635275292	23.96436722394151
24	23.833808568342086	25.816604526129233	26.19473195319549	24.154854952333192
25	24.08809527075146	25.753703089443945	26.413723291416268	23.744478348388323
26	23.852241109921863	25.88620446654208	26.389058499268625	23.87249592426743
27	23.75954998177533	26.109112331304264	26.31011795388997	23.821219733030436
28	23.869177755947632	25.908095052389562	26.36506977420253	23.85765741746027
29	23.640379559601353	26.121056183078345	26.28078285925942	23.957781398060877
30	23.9174815832916	26.228410973097155	26.115810835031823	23.738296608579425
31	23.704139679189073	25.91846141430669	26.373404049432004	24.00399485707223
32	23.465542395603293	26.248331639344947	26.390667072669554	23.895458892382205
33	23.530639107582125	26.16578151742169	26.48357967106694	23.81999970392924
34	23.64669340447217	25.844264994827892	26.53088105411758	23.978160546582362
35	23.336973086818553	26.240385908415213	26.811219651824675	23.61142135294156
36	23.30271902135327	25.892153079771134	26.677164097527573	24.127963801348024
37	23.351430820211966	25.449352454012768	26.942085257420896	24.257131468354366
38	22.918712919031915	25.222112320308458	27.425865650473085	24.433309110186542
39	22.92384947467451	24.615198353224923	27.640007955833077	24.82094421626749
40	23.015390511693802	24.106170351575475	28.248887957073315	24.629551179657405
41	22.624196339827215	23.618314128818987	28.550060300132596	25.207429231221194
42	22.37507339116142	23.045502694923837	28.994539437265853	25.584884476648895
43	21.857769395927455	22.20882694163066	29.725962425201825	26.207441237240058
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1067.0
1	1338.5
2	1610.0
3	4151.0
4	6692.0
5	6692.0
6	9875.0
7	13058.0
8	13943.5
9	14829.0
10	19363.5
11	23898.0
12	23898.0
13	34222.0
14	44546.0
15	65159.0
16	85772.0
17	89966.0
18	94160.0
19	94160.0
20	96628.0
21	99096.0
22	104624.5
23	110153.0
24	154078.0
25	198003.0
26	198003.0
27	286539.5
28	375076.0
29	490849.5
30	606623.0
31	778869.5
32	951116.0
33	951116.0
34	1136547.5
35	1321979.0
36	1458104.5
37	1594230.0
38	1690829.0
39	1787428.0
40	1787428.0
41	1758186.5
42	1728945.0
43	1703158.5
44	1677372.0
45	1674552.5
46	1671733.0
47	1671733.0
48	1702976.5
49	1734220.0
50	1777379.0
51	1820538.0
52	1828581.0
53	1836624.0
54	1836624.0
55	1807011.0
56	1777398.0
57	1680258.5
58	1583119.0
59	1479211.0
60	1375303.0
61	1375303.0
62	1210581.5
63	1045860.0
64	886103.0
65	726346.0
66	620313.0
67	514280.0
68	514280.0
69	422465.5
70	330651.0
71	283404.0
72	236157.0
73	191444.5
74	146732.0
75	146732.0
76	117111.5
77	87491.0
78	70398.0
79	53305.0
80	41934.5
81	30564.0
82	30564.0
83	23970.0
84	17376.0
85	13435.0
86	9494.0
87	6325.0
88	3156.0
89	3156.0
90	1948.5
91	741.0
92	476.5
93	212.0
94	165.5
95	119.0
96	119.0
97	65.5
98	12.0
99	9.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2.5737091E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.133294249104466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99199611466743	30.024012540141054
2	12.927228102649774	10.634789547431712
3	4.861470661823576	5.999049096485334
4	2.477346995882245	4.076057717550375
5	1.5014402527394866	3.087959185669154
6	0.9983134084867817	2.463835151046793
7	0.6798755038233736	1.957586340606748
8	0.5191099989138084	1.7082163466379185
9	0.3776695787819989	1.3981314521677775
>10	2.339256905526075	18.064569815236762
>50	0.19798377731844574	5.646855196411725
>100	0.11618326557179602	8.948697528867482
>500	0.008305193100366974	2.322679241661987
>1k	0.003640787429114729	2.5550048948020176
>5k	7.555927826722338E-5	0.2374824348207673
>10k+	1.0389400761404296E-4	0.8750735104626048
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	43454	0.1688380400100384	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36677	0.14250639281649974	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28446	0.11052531150470735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.885442997423446E-6	0.0	0.0	0.0	7.770885994846893E-5
2	2.7198100981964123E-5	0.0	0.0	3.885442997423446E-6	8.159430294589238E-5
3	4.2739872971657905E-5	0.0	0.0	1.5541771989693785E-5	8.159430294589238E-5
4	4.2739872971657905E-5	0.0	0.0	2.7198100981964123E-5	8.547974594331581E-5
5	1.5541771989693786E-4	0.0	0.0	3.8854429974234465E-5	9.32506319381627E-5
6	2.4089746584025367E-4	0.0	0.0	3.8854429974234465E-5	1.437613909046675E-4
7	2.4866835183510056E-4	0.0	0.0	4.2739872971657905E-5	1.4764683390209096E-4
8	3.0694999679645223E-4	0.0	0.0	4.2739872971657905E-5	1.515322768995144E-4
9	3.22491768786146E-4	0.0	0.0	1.0879240392785649E-4	2.0592847886344266E-4
10	5.789310066160935E-4	0.0	0.0	2.758664528170647E-4	2.2147025085313645E-4
11	6.216708795877514E-4	0.0	0.0	3.3414809777841637E-4	2.4089746584025367E-4
12	6.333272085800217E-4	0.0	0.0	3.9242974273976804E-4	3.458044267706867E-4
13	6.644107525594093E-4	3.885442997423446E-6	0.0	4.118569577268853E-4	4.040860717320384E-4
14	7.382341695104548E-4	7.770885994846893E-6	0.0	4.390550587088494E-4	4.584822736959666E-4
15	8.081721434640768E-4	7.770885994846893E-6	0.0	5.089930326624715E-4	5.944727786057872E-4
16	8.664537884254285E-4	7.770885994846893E-6	0.0	7.732031564872658E-4	6.333272085800217E-4
17	8.819955604151223E-4	7.770885994846893E-6	0.0	0.0010995803682708352	6.488689805697155E-4
18	8.897664464099692E-4	7.770885994846893E-6	0.0	0.001810616436799326	7.343487265130313E-4
19	9.05308218399663E-4	7.770885994846893E-6	0.0	0.002420630987394807	7.537759415001486E-4
20	0.0010879240392785649	1.5541771989693785E-5	0.0	0.004681958811895252	8.237139154537706E-4
21	0.001620229729925577	1.5541771989693785E-5	0.0	0.008066179662651074	8.470265734383112E-4
22	0.0016474278309075413	1.5541771989693785E-5	0.0	0.012309083415837477	8.664537884254285E-4
23	0.0016590841598998116	1.5541771989693785E-5	0.0	0.014760797947211673	9.40277205376474E-4
24	0.001690167703879199	1.5541771989693785E-5	0.0	0.019683654224947177	9.519335343687443E-4
25	0.0017095949188663164	1.5541771989693785E-5	0.0	0.0232504908965819	9.597044203635912E-4
26	0.0018611271957658308	1.5541771989693785E-5	0.0	0.03313117243902972	0.001017986065324943
27	0.001915523397729759	1.5541771989693785E-5	0.0	0.1080463988723512	0.0010568404952991774
28	0.00195049238470657	1.5541771989693785E-5	0.0	0.2726182224712187	0.0010684968242914478
29	0.00195049238470657	1.5541771989693785E-5	0.0	0.46713904069422607	0.0010801531532837181
30	0.001954377827703993	1.5541771989693785E-5	0.0	0.6444862008686219	0.0010801531532837181
31	0.0019738050426911105	1.5541771989693785E-5	0.0	0.7746563121683021	0.0010879240392785649
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19410	0.0	25.295723	1
GTATCAA	39895	0.0	18.05715	1
CTACTCG	6960	0.0	12.838361	14
AACGCAG	55585	0.0	12.750472	6
TATCAAC	56100	0.0	12.709268	2
ATCAACG	56230	0.0	12.587763	3
TCAACGC	56335	0.0	12.584005	4
CAACGCA	56820	0.0	12.4668255	5
TAGTCCC	7615	0.0	12.3657255	5
TACTCGG	7200	0.0	12.281943	15
ACGCAGA	58575	0.0	12.068031	7
CGCAGAG	60100	0.0	11.764891	8
ATCTCCG	7985	0.0	11.746399	10
ACGAGAC	7800	0.0	11.645513	22
TAGCGCA	4940	0.0	11.459514	4
AGAGTAC	61550	0.0	11.457677	11
CGTATAC	635	0.0	11.362204	3
CTGTCTC	119675	0.0	11.227532	37
AGACCGA	8110	0.0	11.223181	25
CCCACGA	8245	0.0	11.196482	19
>>END_MODULE