FastQCFastQC Report
Thu 26 May 2016
SRR1041762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30361983
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT823660.2712800412278737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT723860.23840998791152737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT561690.184997798068723No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGT548260.1805745033188379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA353850.024.3372921
GTATCAA662850.016.7626171
ACGCTAC139400.014.93005827
GACGCTA142800.014.60049126
CGCTATC142300.014.57378733
TACGCTA142150.014.56313831
TATCTCG143550.014.48554636
GCTACGC144200.014.381762529
CGCTACG145000.014.28965728
ATCTCCG150500.014.20996810
ACGCTAT147000.014.15816432
CTACGCT148200.013.956140530
ACGAGAC153850.013.68410922
CCCACGA159300.013.33207919
CACGAGA158800.013.21095821
ATCTCGT156200.013.18213937
CGAGACG161700.013.06555423
CCGTCTT39100.013.0115137
AGACGCT162750.012.89032325
TCTCCGA167900.012.70428811
Produced by FastQC (version 0.11.5)