##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041762_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30361983
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16682484144728	33.0	31.0	34.0	30.0	34.0
2	32.258860134398994	34.0	31.0	34.0	30.0	34.0
3	32.37491283095706	34.0	31.0	34.0	30.0	34.0
4	35.92465683153831	37.0	35.0	37.0	35.0	37.0
5	35.87262758825733	37.0	35.0	37.0	35.0	37.0
6	35.93177230881132	37.0	35.0	37.0	35.0	37.0
7	35.866384715385685	37.0	35.0	37.0	35.0	37.0
8	35.856681297792704	37.0	35.0	37.0	35.0	37.0
9	37.58208371963057	39.0	37.0	39.0	35.0	39.0
10	37.4768791946165	39.0	37.0	39.0	34.0	39.0
11	37.563244041075976	39.0	37.0	39.0	35.0	39.0
12	37.47518889000103	39.0	37.0	39.0	34.0	39.0
13	37.491397580981456	39.0	37.0	39.0	35.0	39.0
14	38.33991202089798	40.0	38.0	40.0	34.0	40.0
15	38.326296902280724	40.0	38.0	40.0	34.0	40.0
16	38.29127639653839	40.0	38.0	40.0	34.0	40.0
17	38.27134140085646	40.0	38.0	40.0	34.0	40.0
18	38.22097851777336	40.0	38.0	40.0	34.0	40.0
19	38.22705783084063	40.0	38.0	40.0	34.0	40.0
20	38.16217165393973	40.0	38.0	40.0	34.0	40.0
21	38.105735781487	40.0	38.0	40.0	34.0	40.0
22	38.0580692308536	40.0	38.0	40.0	34.0	40.0
23	37.979668752202386	40.0	38.0	40.0	34.0	40.0
24	37.916304511467516	40.0	38.0	40.0	33.0	40.0
25	37.883447994816414	40.0	38.0	40.0	33.0	40.0
26	37.740796113350044	40.0	38.0	40.0	33.0	40.0
27	37.65829708817108	40.0	37.0	40.0	33.0	40.0
28	37.50585803305403	40.0	37.0	40.0	32.0	40.0
29	37.43603660538246	40.0	37.0	40.0	32.0	40.0
30	37.33338599787768	40.0	37.0	40.0	32.0	40.0
31	37.259926698463666	40.0	37.0	40.0	32.0	40.0
32	37.11079006269123	40.0	36.0	40.0	31.0	40.0
33	37.185992067777654	40.0	37.0	40.0	31.0	40.0
34	37.10219638816081	40.0	36.0	40.0	31.0	40.0
35	37.245792344986164	40.0	37.0	40.0	32.0	40.0
36	37.28563401145439	40.0	37.0	40.0	32.0	40.0
37	37.25267651325672	40.0	37.0	40.0	32.0	40.0
38	37.20930414854656	40.0	37.0	40.0	32.0	40.0
39	37.16638534446186	40.0	37.0	40.0	32.0	40.0
40	37.13469545121608	40.0	37.0	40.0	31.0	40.0
41	37.10068578195304	40.0	37.0	40.0	31.0	40.0
42	37.062149893173974	40.0	37.0	40.0	31.0	40.0
43	36.755385180210396	40.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	5.0
12	12.0
13	23.0
14	42.0
15	64.0
16	120.0
17	269.0
18	674.0
19	1693.0
20	3934.0
21	8716.0
22	17597.0
23	32777.0
24	55848.0
25	90356.0
26	136147.0
27	197737.0
28	277020.0
29	374623.0
30	492468.0
31	627026.0
32	782748.0
33	981707.0
34	1261565.0
35	1657732.0
36	2346587.0
37	3889102.0
38	1.2108183E7
39	5017202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81519754490344	19.482640511326284	12.507414288454083	25.194747655316192
2	18.13523181275742	22.138843829798603	34.55839165709302	25.167532700350964
3	19.24551502449626	23.654762602297748	30.87853649084778	26.22118588235821
4	14.597910156263508	16.658546314316823	35.352457051306565	33.39108647811311
5	14.467665040191873	35.908145393533744	34.67585763419998	14.948331932074396
6	33.025260570101764	36.004288652687805	15.802311067758652	15.168139709451783
7	28.28836311514963	31.400234958302953	21.415465518177783	18.895936408369636
8	26.786511276289165	33.71883516303925	20.374924127979387	19.119729432692193
9	25.714865198363363	15.164378426797748	20.3697498941357	38.75100648070319
10	16.751191778218175	27.821670936315325	32.34519629366764	23.081940991798856
11	35.33378567532957	22.465396940641195	21.86149369756251	20.339323686466724
12	22.127830056422862	25.368418788720092	29.250342443047938	23.253408711809108
13	29.944855709852682	20.603044932868844	24.789365701179662	24.662733656098812
14	21.86844976495771	20.9975053342201	26.133829928038626	31.000214972783564
15	24.36448897293698	28.402627720330386	23.183050988468047	24.04983231826459
16	24.11221296053028	27.534772679373415	24.52933657198873	23.82367778810758
17	23.107416929915285	27.32078138638046	25.850636962677964	23.72116472102629
18	24.13900962924589	25.9418958241298	26.759006485182475	23.160088061441837
19	25.019584524502235	25.78371116273927	26.49694850300127	22.69975580975722
20	24.52240355974114	25.81938076969478	26.55404292927771	23.104172741286362
21	24.402984482271794	26.0740907469713	26.211305104808208	23.311619665948697
22	24.263250526159638	26.001595482086927	26.054408238091696	23.680745753661743
23	23.963965067762537	25.923362779038513	26.236761281369535	23.87591087182942
24	23.767235493149443	25.87693629892356	26.405241712967165	23.950586494959833
25	23.864910272823746	25.779007912625467	26.6615754313544	23.69450638319638
26	23.675169701531022	25.924037965504425	26.749675737582752	23.651116595381797
27	23.544410785026788	26.17994022327198	26.509800101001307	23.765848890699925
28	23.599390724907526	25.928481021809414	26.633085856085227	23.839042397197836
29	23.514518139345512	26.226057105690366	26.50544926528679	23.753975489677337
30	23.50802317490264	26.105195434698715	26.437970141805295	23.948811248593348
31	23.538116729727435	25.85736906578203	26.8143981241278	23.790116080362736
32	23.112795366495	26.49536757859327	26.596974907732474	23.794862147179256
33	23.153507463593535	26.119585140404038	26.78696908564898	23.939938310353444
34	23.505411356036923	25.902955679805235	26.791339682918604	23.80029328123924
35	22.962126683227506	26.119944141988356	27.13250317016514	23.785426004619
36	22.822695737626887	25.68760083951038	27.31758001445426	24.17212340840847
37	22.904818173437487	25.362832855811824	27.50246912396993	24.229879846780758
38	22.485135440593588	24.981438135974187	28.120600686720625	24.412825736711596
39	22.525804720989402	24.331091944817963	28.390540235794216	24.752563098398415
40	22.494604519079004	23.77475805845751	29.035784652142123	24.69485277032136
41	22.088613250326897	23.18768177954648	29.284747310477055	25.43895765964957
42	21.735082323180276	22.544864740883362	29.89038298321951	25.829669952716856
43	21.055966601390956	21.63333666315537	30.82936974175896	26.481326993694715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5170.0
1	5001.0
2	4832.0
3	9969.5
4	15107.0
5	15107.0
6	21386.0
7	27665.0
8	29941.0
9	32217.0
10	41328.0
11	50439.0
12	50439.0
13	70731.5
14	91024.0
15	130697.5
16	170371.0
17	175702.5
18	181034.0
19	181034.0
20	180899.5
21	180765.0
22	187493.0
23	194221.0
24	267744.5
25	341268.0
26	341268.0
27	476468.0
28	611668.0
29	749019.0
30	886370.0
31	1058936.5
32	1231503.0
33	1231503.0
34	1392532.5
35	1553562.0
36	1634649.5
37	1715737.0
38	1742487.0
39	1769237.0
40	1769237.0
41	1720536.0
42	1671835.0
43	1681537.5
44	1691240.0
45	1758051.5
46	1824863.0
47	1824863.0
48	1908179.5
49	1991496.0
50	2056844.0
51	2122192.0
52	2183392.5
53	2244593.0
54	2244593.0
55	2154079.5
56	2063566.0
57	1993262.5
58	1922959.0
59	1790415.5
60	1657872.0
61	1657872.0
62	1466846.0
63	1275820.0
64	1093252.5
65	910685.0
66	783920.0
67	657155.0
68	657155.0
69	546742.0
70	436329.0
71	381909.5
72	327490.0
73	268067.5
74	208645.0
75	208645.0
76	168249.0
77	127853.0
78	101450.0
79	75047.0
80	60363.5
81	45680.0
82	45680.0
83	35211.0
84	24742.0
85	19156.5
86	13571.0
87	9033.5
88	4496.0
89	4496.0
90	2817.0
91	1138.0
92	740.5
93	343.0
94	245.5
95	148.0
96	148.0
97	85.0
98	22.0
99	17.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3.0361983E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.531827706065854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.98530359335399	29.17695922614667
2	13.445413672632295	10.899343808318287
3	5.200545088762273	6.323627925460183
4	2.6013275616107223	4.217462421369849
5	1.5565119135012766	3.1544136350236314
6	0.9970680956505718	2.42477953584745
7	0.7018619280032644	1.9913422717492857
8	0.4954021848464246	1.6063644801123096
9	0.39742148428736923	1.4497397215042147
>10	2.3062147658388814	17.389018610634956
>50	0.18301208671202052	5.139431955083236
>100	0.11634455039871701	8.963774171408911
>500	0.009219303818136328	2.553086779978078
>1k	0.004118040573121238	2.9675595723456576
>5k	9.754345296472516E-5	0.24494947660862054
>10k+	1.3818655818125433E-4	1.498146408408743
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	82366	0.2712800412278737	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	72386	0.23840998791152737	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	56169	0.184997798068723	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGT	54826	0.1805745033188379	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.880777550003898E-6	0.0	0.0	6.587185033335932E-6	8.563340543336712E-5
2	6.916544285002729E-5	0.0	0.0	1.9761555100007795E-5	8.563340543336712E-5
3	1.0539496053337491E-4	0.0	0.0	2.9642332650011695E-5	8.892699795003508E-5
4	1.0868855305004288E-4	0.0	0.0	3.622951768334763E-5	8.892699795003508E-5
5	3.2606565915012863E-4	1.3174370066671864E-5	0.0	4.281670271668356E-5	1.1527573808337881E-4
6	4.545157673001793E-4	1.3174370066671864E-5	0.0	4.281670271668356E-5	1.7126681086673424E-4
7	5.170940251168707E-4	1.3174370066671864E-5	0.0	4.940388775001949E-5	1.8114758841673812E-4
8	6.422505407502534E-4	1.3174370066671864E-5	0.0	5.2697480266687456E-5	1.844411809334061E-4
9	6.718928734002651E-4	1.3174370066671864E-5	0.0	9.880777550003899E-5	2.2396429113342168E-4
10	0.0010407752352670772	1.3174370066671864E-5	0.0	3.063041040501208E-4	2.338450686834256E-4
11	0.0010967663080504327	1.646796258333983E-5	0.0	4.281670271668356E-4	2.5031303126676544E-4
12	0.0011461701958004522	2.9642332650011695E-5	0.0	4.512221747835113E-4	3.4912080676680437E-4
13	0.0012318036012338193	3.952311020001559E-5	0.0	4.742773224001871E-4	3.91937509483488E-4
14	0.0014392999297839011	3.952311020001559E-5	0.0	5.072132475668668E-4	4.3475421220017153E-4
15	0.0015315205202506043	4.611029523335152E-5	0.0	6.257825781669135E-4	5.203876176335386E-4
16	0.0016072731481339673	4.611029523335152E-5	0.0	0.0010473624203004132	5.401491727335464E-4
17	0.0016204475182006392	4.611029523335152E-5	0.0	0.0016566770358839868	5.533235428002183E-4
18	0.001636915480783979	5.928466530002339E-5	0.0	0.0026875714936010603	6.323697632002494E-4
19	0.0016863193685339986	5.928466530002339E-5	0.0	0.0037184659513181334	6.356633557169175E-4
20	0.00192675162225076	7.245903536669525E-5	0.0	0.006435679777569206	7.212967611502846E-4
21	0.0027666177140010916	7.904622040003118E-5	0.0	0.011679079064104607	7.443519087669603E-4
22	0.002799553639167771	7.904622040003118E-5	0.0	0.01765036229682363	7.904622040003119E-4
23	0.0028193151942677787	8.233981291669915E-5	0.0	0.02200449160385868	8.266917216836595E-4
24	0.0028555447119511263	8.233981291669915E-5	0.0	0.029296505435761556	8.299853142003274E-4
25	0.0028851870446011383	8.563340543336712E-5	0.0	0.03567619414054741	8.365724992336634E-4
26	0.0030498666704345365	8.892699795003508E-5	0.0	0.05248669034562071	8.826827944670149E-4
27	0.0031256192983178995	9.222059046670305E-5	0.0	0.17046317429266725	9.090315346003586E-4
28	0.003178316778584587	9.222059046670305E-5	0.0	0.3834071048653179	9.189123121503626E-4
29	0.003184903963617923	9.222059046670305E-5	0.0	0.6528756702090243	9.254994971836985E-4
30	0.003188197556134591	1.0868855305004288E-4	0.0	0.9047795066613402	9.287930897003664E-4
31	0.003204665518717931	1.0868855305004288E-4	0.0	1.084958778878178	9.518482373170421E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	35385	0.0	24.337292	1
GTATCAA	66285	0.0	16.762617	1
ACGCTAC	13940	0.0	14.930058	27
GACGCTA	14280	0.0	14.600491	26
CGCTATC	14230	0.0	14.573787	33
TACGCTA	14215	0.0	14.563138	31
TATCTCG	14355	0.0	14.485546	36
GCTACGC	14420	0.0	14.3817625	29
CGCTACG	14500	0.0	14.289657	28
ATCTCCG	15050	0.0	14.209968	10
ACGCTAT	14700	0.0	14.158164	32
CTACGCT	14820	0.0	13.9561405	30
ACGAGAC	15385	0.0	13.684109	22
CCCACGA	15930	0.0	13.332079	19
CACGAGA	15880	0.0	13.210958	21
ATCTCGT	15620	0.0	13.182139	37
CGAGACG	16170	0.0	13.065554	23
CCGTCTT	3910	0.0	13.01151	37
AGACGCT	16275	0.0	12.890323	25
TCTCCGA	16790	0.0	12.704288	11
>>END_MODULE