FastQCFastQC Report
Thu 26 May 2016
SRR1041761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33888276
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT832450.24564542616449417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT742180.21900789523786926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT561800.16578004735324983No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT352890.1041333586872345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA398150.022.8560831
GTATCAA734400.016.4595581
ATCTCCG110000.013.26954510
ACGAGAC114050.012.74967122
TAGCGCA60350.012.4150794
AACGCAG974650.012.159346
ATCTCGT117600.012.05016937
CACGAGA119350.011.99748521
TATCAAC1009450.011.9014312
TCTCCGA124800.011.68108811
ATCAACG1025500.011.6592393
CTACTCG87650.011.65088414
CCCACGA125450.011.59107219
TCAACGC1032650.011.5910524
TATACAG72750.011.5704465
CAACGCA1034900.011.5676395
AACCGGC85450.011.51784516
TGAACCG86700.011.47981514
GATCTCG125900.011.476171536
ACGCAGA1037300.011.4142487
Produced by FastQC (version 0.11.5)