FastQCFastQC Report
Thu 26 May 2016
SRR1041760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28938343
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1666940.5760315993213572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1445290.4994377183240934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1186950.4101651570029424No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT487770.1685549169142131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA467450.023.2867681
GTATCAA865450.016.7268471
AACGCAG1148300.012.4697386
TATCAAC1198200.012.0476972
ATCAACG1220150.011.8339963
CTGTCTC1314450.011.81399137
TTAGGCA87650.011.7775244
TCAACGC1230200.011.7358154
CAACGCA1236800.011.683665
TGTCTCT840400.011.68247337
TAGCGCA53150.011.5211674
ACGCAGA1272050.011.2420897
TATACTG40800.011.1997555
TGAACCG67950.011.18984614
CGCAGAG1284250.011.1136858
AACCGGC67750.011.08634816
TATACAC150200.011.04826837
TAGTCCC76100.010.9152435
TAAGGCG18950.010.8364115
AGAGTAC1318900.010.75998911
Produced by FastQC (version 0.11.5)