##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041760_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28938343
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15609197112634	33.0	31.0	34.0	30.0	34.0
2	32.25235211290432	34.0	31.0	34.0	30.0	34.0
3	32.36280249356364	34.0	31.0	34.0	30.0	34.0
4	35.91972812679703	37.0	35.0	37.0	35.0	37.0
5	35.88004130022234	37.0	35.0	37.0	35.0	37.0
6	35.941934926958325	37.0	35.0	37.0	35.0	37.0
7	35.87918966196509	37.0	35.0	37.0	35.0	37.0
8	35.87453144777501	37.0	35.0	37.0	35.0	37.0
9	37.59026389313306	39.0	37.0	39.0	35.0	39.0
10	37.49607515537431	39.0	37.0	39.0	35.0	39.0
11	37.568121333000995	39.0	37.0	39.0	35.0	39.0
12	37.488356192336234	39.0	37.0	39.0	35.0	39.0
13	37.50131491633781	39.0	37.0	39.0	35.0	39.0
14	38.34776555796578	40.0	38.0	40.0	35.0	40.0
15	38.32597927255199	40.0	38.0	40.0	34.0	40.0
16	38.29562646347789	40.0	38.0	40.0	34.0	40.0
17	38.29166148179252	40.0	38.0	40.0	34.0	40.0
18	38.25451298299975	40.0	38.0	40.0	34.0	40.0
19	38.26130608100125	40.0	38.0	40.0	34.0	40.0
20	38.20127081913432	40.0	38.0	40.0	34.0	40.0
21	38.14450613153628	40.0	38.0	40.0	34.0	40.0
22	38.09181303158927	40.0	38.0	40.0	34.0	40.0
23	38.01536425219647	40.0	38.0	40.0	34.0	40.0
24	37.95285697595056	40.0	38.0	40.0	33.0	40.0
25	37.91748712080716	40.0	38.0	40.0	33.0	40.0
26	37.774146156191456	40.0	38.0	40.0	33.0	40.0
27	37.68806513904407	40.0	37.0	40.0	33.0	40.0
28	37.538042451152094	40.0	37.0	40.0	33.0	40.0
29	37.46516167148893	40.0	37.0	40.0	32.0	40.0
30	37.35596879199338	40.0	37.0	40.0	32.0	40.0
31	37.267874909078245	40.0	37.0	40.0	32.0	40.0
32	37.11044986231589	40.0	36.0	40.0	32.0	40.0
33	37.162581492658376	40.0	36.0	40.0	32.0	40.0
34	37.06628333902878	40.0	36.0	40.0	31.0	40.0
35	37.173325335179	40.0	36.0	40.0	32.0	40.0
36	37.18632566487998	40.0	37.0	40.0	32.0	40.0
37	37.13778919546292	40.0	36.0	40.0	32.0	40.0
38	37.07463164010462	40.0	36.0	40.0	31.0	40.0
39	37.012032686183865	40.0	36.0	40.0	31.0	40.0
40	36.957564294541676	40.0	36.0	40.0	31.0	40.0
41	36.89424200964098	40.0	36.0	40.0	31.0	40.0
42	36.82716501770678	40.0	36.0	40.0	31.0	40.0
43	36.49890924991801	40.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	12.0
12	15.0
13	12.0
14	19.0
15	46.0
16	98.0
17	258.0
18	689.0
19	1719.0
20	4030.0
21	8664.0
22	17372.0
23	32039.0
24	54834.0
25	87912.0
26	131854.0
27	191942.0
28	269354.0
29	365989.0
30	478753.0
31	605135.0
32	752604.0
33	940395.0
34	1207289.0
35	1584192.0
36	2258845.0
37	3813358.0
38	1.1275346E7
39	4855562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2104457397578	19.522071460691443	12.568756960272399	24.698725839278357
2	18.231434329187405	22.367227453209743	34.3469182046809	25.05442001292196
3	19.467987507093962	23.88454998961067	30.76159198195971	25.88587052133565
4	14.57827422945398	16.637338219399776	35.28291858314071	33.501468968005526
5	14.369184856230365	36.23488393927738	34.27667921414851	15.119251990343747
6	32.86421755385234	36.12668147585368	15.67990261225392	15.329198358040058
7	28.06706313488647	31.875370334783852	21.362733173768795	18.694833356560878
8	27.106907952538954	33.53573838004477	20.12202979279083	19.23532387462544
9	25.9389316105625	14.782650133077766	20.361943322048536	38.9164749343112
10	16.729219775990632	27.884920708832567	32.23912993221485	23.146729582961957
11	35.18463721298763	22.730067854956314	21.913614749814805	20.171680182241257
12	22.26195190236013	25.616072765465525	29.436982621983574	22.684992710190766
13	29.947461055389386	20.772682112448525	25.04642715721491	24.23342967494718
14	21.856520948694264	21.05289857128309	26.7176838701511	30.372896609871546
15	24.201537731445093	28.50919280347185	23.754936486861048	23.534332978222007
16	23.53386992475692	27.84452793306099	24.996434661099983	23.62516748108211
17	22.7808827893152	27.374594322833207	26.29919411764523	23.545328770206368
18	23.625347173471543	26.01156189212354	27.463728659239408	22.899362275165515
19	24.576196363419978	25.833220651230793	27.514125463230567	22.076457522118666
20	24.054369664496686	25.986619206220617	27.523389988155163	22.435621141127534
21	23.81762148579136	26.149641670913915	27.28596796298945	22.746768880305275
22	23.740540361968893	25.993744700586348	27.049351097953327	23.216363839491432
23	23.287618783148712	26.11840975138072	27.331184788292816	23.26278667717775
24	23.043368447184413	26.092520224810382	27.438706494010383	23.425404833994815
25	23.28043454319413	25.946796608223217	27.646855937812337	23.125912910770325
26	22.989844304492486	26.234777851655156	27.72820821150679	23.04716963234557
27	23.03022671339544	26.36797137970201	27.51611590200586	23.085686004896687
28	23.06956897981339	26.41510607570033	27.461679474875254	23.053645469611027
29	22.826109981487193	26.60441200797157	27.48743423215351	23.082043778387725
30	22.93700783075244	26.64429335155783	27.230263322264165	23.188435495425566
31	22.9941119987416	26.38218090095898	27.38197553329159	23.24173156700783
32	22.62330984189385	26.766694969369876	27.28577099248564	23.32422419625063
33	22.6490265873205	26.7063770721081	27.405377011392808	23.239219329178592
34	22.904504242001693	26.515678523818725	27.53701550914646	23.042801725033115
35	22.552808224023053	26.77541696150329	27.641651769764426	23.030123044709228
36	22.41985313395449	26.43216303020529	27.66825315464676	23.479730681193463
37	22.527481963981145	26.014163976147493	27.865140723503067	23.59321333636829
38	22.174693969174392	25.868851578682307	28.285838619025284	23.67061583311802
39	22.13031340460648	25.386774909676063	28.590856083224946	23.892055602492512
40	22.29803551640811	24.999036745123934	28.887317425189135	23.81561031327882
41	21.86858798376949	24.454541160148665	29.269143019004236	24.407727837077612
42	21.595459007449044	23.997642159400765	29.600357560209993	24.806541272940198
43	21.0851429883183	23.22782614056375	30.25911677112957	25.42791409998838
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10619.0
1	10244.0
2	9869.0
3	20612.0
4	31355.0
5	31355.0
6	42664.0
7	53973.0
8	56238.5
9	58504.0
10	75384.5
11	92265.0
12	92265.0
13	134098.5
14	175932.0
15	254213.0
16	332494.0
17	341988.5
18	351483.0
19	351483.0
20	345996.0
21	340509.0
22	329199.0
23	317889.0
24	401142.5
25	484396.0
26	484396.0
27	597535.5
28	710675.0
29	794711.5
30	878748.0
31	963787.5
32	1048827.0
33	1048827.0
34	1115820.0
35	1182813.0
36	1233021.0
37	1283229.0
38	1333873.0
39	1384517.0
40	1384517.0
41	1413005.0
42	1441493.0
43	1513227.0
44	1584961.0
45	1681797.5
46	1778634.0
47	1778634.0
48	1877520.5
49	1976407.0
50	2047786.0
51	2119165.0
52	2116702.5
53	2114240.0
54	2114240.0
55	2051248.0
56	1988256.0
57	1898045.5
58	1807835.0
59	1685828.0
60	1563821.0
61	1563821.0
62	1379447.5
63	1195074.0
64	1026099.0
65	857124.0
66	735743.0
67	614362.0
68	614362.0
69	510925.5
70	407489.0
71	350609.5
72	293730.0
73	240210.0
74	186690.0
75	186690.0
76	150823.0
77	114956.0
78	90671.5
79	66387.0
80	53788.5
81	41190.0
82	41190.0
83	31420.5
84	21651.0
85	16775.5
86	11900.0
87	7711.5
88	3523.0
89	3523.0
90	2237.5
91	952.0
92	625.0
93	298.0
94	194.5
95	91.0
96	91.0
97	53.0
98	15.0
99	8.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2.8938343E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.02144752200296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.53182407378318	27.912753192482782
2	13.305393611090702	10.383914371095383
3	5.281505362968505	6.182759530747581
4	2.6159819465877097	4.083176089891179
5	1.59148444388179	3.105101335450867
6	1.042997848829019	2.441957149418612
7	0.7167060132487938	1.9576834259284235
8	0.5532031635194001	1.7269430571422633
9	0.41488847325935635	1.457059390809651
>10	2.5834169090524983	18.830047435307197
>50	0.22378029218787	6.029078006111342
>100	0.1281591239970949	9.14649322884738
>500	0.006923846765721385	1.8504117663505268
>1k	0.0035049001779562036	2.509511753832993
>5k	1.0614953116077165E-4	0.280092818983434
>10k+	1.238411195847421E-4	2.1030174476003336
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	166694	0.5760315993213572	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	144529	0.4994377183240934	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	118695	0.4101651570029424	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48777	0.1685549169142131	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4556228737768434E-6	0.0	0.0	3.4556228737768434E-6	7.94793260968674E-5
2	1.036686862133053E-4	6.911245747553687E-6	0.0	6.911245747553687E-6	7.94793260968674E-5
3	1.5204740644618112E-4	6.911245747553687E-6	0.0	6.911245747553687E-6	8.293494897064424E-5
4	1.5550302931995795E-4	6.911245747553687E-6	0.0	6.911245747553687E-6	8.984619471819793E-5
5	3.97396630484337E-4	6.911245747553687E-6	0.0	1.7278114368884215E-5	1.0712430908708214E-4
6	5.183434310665265E-4	6.911245747553687E-6	0.0	2.4189360116437902E-5	1.347692920772969E-4
7	5.94367134289617E-4	6.911245747553687E-6	0.0	2.7644982990214747E-5	1.347692920772969E-4
8	7.256808034931371E-4	6.911245747553687E-6	0.0	3.110060586399159E-5	1.3822491495107372E-4
9	7.70603900852236E-4	6.911245747553687E-6	0.0	5.5289965980429495E-5	1.6241427506751165E-4
10	0.0011334443025988047	1.036686862133053E-5	0.0	1.6241427506751165E-4	1.6586989794128848E-4
11	0.0012440242345596636	1.036686862133053E-5	0.0	2.1424861817416428E-4	1.7278114368884218E-4
12	0.0013096810691614236	1.036686862133053E-5	0.0	2.4189360116437904E-4	2.4189360116437904E-4
13	0.0013995272638796215	1.036686862133053E-5	0.0	2.4189360116437904E-4	2.729942070283706E-4
14	0.0016068646363062323	1.036686862133053E-5	0.0	2.5917171553326327E-4	3.351954187563538E-4
15	0.0017105333225195375	2.7644982990214747E-5	0.0	3.0409481289236223E-4	3.97396630484337E-4
16	0.0017934682714901818	2.7644982990214747E-5	0.0	5.632665284256255E-4	4.354084820958823E-4
17	0.0018107463858590658	3.455622873776843E-5	0.0	8.673613413179877E-4	4.457753507172128E-4
18	0.001831480123101727	3.455622873776843E-5	0.0	0.0013615154122680762	4.906984480763118E-4
19	0.0018556694832181648	3.455622873776843E-5	0.0	0.001872947597587049	4.976096938238654E-4
20	0.0021701311647318576	3.455622873776843E-5	0.0	0.0036111259030968015	5.73633397046956E-4
21	0.003220640518360018	3.455622873776843E-5	0.0	0.006990725073650554	6.047340029109476E-4
22	0.0032482855013502327	3.801185161154528E-5	0.0	0.010484359799038943	6.39290231648716E-4
23	0.003286297352961778	3.801185161154528E-5	0.0	0.0130276982341387	7.014914433766992E-4
24	0.003307031090204439	4.492309735909896E-5	0.0	0.01703276514484606	7.118583119980297E-4
25	0.003376143547679976	4.492309735909896E-5	0.0	0.020052979536527023	7.153139348718066E-4
26	0.003600759034475471	4.492309735909896E-5	0.0	0.028028557129203976	7.636926551046824E-4
27	0.0037182502121838835	4.492309735909896E-5	0.0	0.0855543111089671	7.844263923473435E-4
28	0.0037804514239118668	4.492309735909896E-5	6.911245747553687E-6	0.19691521383929964	7.844263923473435E-4
29	0.00378736266965942	4.492309735909896E-5	6.911245747553687E-6	0.34341634557306894	7.844263923473435E-4
30	0.0038046407840283047	4.492309735909896E-5	6.911245747553687E-6	0.482550089339946	7.844263923473435E-4
31	0.0038288301441447427	4.492309735909896E-5	6.911245747553687E-6	0.5846430115228091	8.086157524637813E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	46745	0.0	23.286768	1
GTATCAA	86545	0.0	16.726847	1
AACGCAG	114830	0.0	12.469738	6
TATCAAC	119820	0.0	12.047697	2
ATCAACG	122015	0.0	11.833996	3
CTGTCTC	131445	0.0	11.813991	37
TTAGGCA	8765	0.0	11.777524	4
TCAACGC	123020	0.0	11.735815	4
CAACGCA	123680	0.0	11.68366	5
TGTCTCT	84040	0.0	11.682473	37
TAGCGCA	5315	0.0	11.521167	4
ACGCAGA	127205	0.0	11.242089	7
TATACTG	4080	0.0	11.199755	5
TGAACCG	6795	0.0	11.189846	14
CGCAGAG	128425	0.0	11.113685	8
AACCGGC	6775	0.0	11.086348	16
TATACAC	15020	0.0	11.048268	37
TAGTCCC	7610	0.0	10.915243	5
TAAGGCG	1895	0.0	10.836411	5
AGAGTAC	131890	0.0	10.759989	11
>>END_MODULE