FastQCFastQC Report
Thu 26 May 2016
SRR1041759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25457814
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2618681.0286350587682038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2594861.0192784030867694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1911020.7506614668486461No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT937820.3683819828363897No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT315720.12401693248289111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA971850.016.062461
GTATCAA1528650.012.76901151
TACACCG15150.012.211225
CTACTCG56400.011.93971614
TATCTCG47700.011.79035736
TATACAC144300.011.537
TACTCGG58550.011.438086515
AACGCAG1642650.011.3918066
TATACAG48000.011.3312495
ACGGACT49500.011.28686827
TATACTG32900.011.0775085
CTGTCTC1027200.011.00418737
TAGTCCC66100.010.8313155
TACACAG67450.010.7516695
TGTCTCT673750.010.68675237
TTAGGCA68050.010.6568694
ACGAGAC57400.010.53919922
TAGCGCA43750.010.4868574
CGAGACG58400.010.3904123
TGAACCG55650.010.37196714
Produced by FastQC (version 0.11.5)