##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041759_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25457814
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.041331239202236	33.0	31.0	34.0	30.0	34.0
2	32.13522791077035	33.0	31.0	34.0	30.0	34.0
3	32.22716636235931	34.0	31.0	34.0	30.0	34.0
4	35.81329154969865	37.0	35.0	37.0	35.0	37.0
5	35.77632525714895	37.0	35.0	37.0	35.0	37.0
6	35.858961338942926	37.0	35.0	37.0	35.0	37.0
7	35.78955204087829	37.0	35.0	37.0	33.0	37.0
8	35.79712075828663	37.0	35.0	37.0	33.0	37.0
9	37.48117799902222	39.0	37.0	39.0	35.0	39.0
10	37.376177506835425	39.0	37.0	39.0	34.0	39.0
11	37.46631871063242	39.0	37.0	39.0	34.0	39.0
12	37.38302860567683	39.0	37.0	39.0	34.0	39.0
13	37.400683774341346	39.0	37.0	39.0	34.0	39.0
14	38.20117889933519	40.0	38.0	40.0	34.0	40.0
15	38.18159838861263	40.0	38.0	40.0	34.0	40.0
16	38.15396439772873	40.0	38.0	40.0	34.0	40.0
17	38.17908957147695	40.0	38.0	40.0	34.0	40.0
18	38.158403899093614	40.0	38.0	40.0	34.0	40.0
19	38.17239901273534	40.0	38.0	40.0	34.0	40.0
20	38.113533078684604	40.0	38.0	40.0	34.0	40.0
21	38.05270939602277	40.0	38.0	40.0	34.0	40.0
22	37.99102271703297	40.0	38.0	40.0	34.0	40.0
23	37.90386660849985	40.0	38.0	40.0	33.0	40.0
24	37.83550382605514	40.0	38.0	40.0	33.0	40.0
25	37.78972927526299	40.0	37.0	40.0	33.0	40.0
26	37.61303225013742	40.0	37.0	40.0	33.0	40.0
27	37.50498856657528	40.0	37.0	40.0	33.0	40.0
28	37.33474775171191	40.0	37.0	40.0	32.0	40.0
29	37.23943088750668	40.0	36.0	40.0	32.0	40.0
30	37.092959827579854	39.0	36.0	40.0	32.0	40.0
31	36.948965924568384	39.0	36.0	40.0	31.0	40.0
32	36.751107577422005	39.0	35.0	40.0	31.0	40.0
33	36.75899085443864	39.0	35.0	40.0	31.0	40.0
34	36.62877366454166	39.0	35.0	40.0	31.0	40.0
35	36.67227457157162	39.0	35.0	40.0	31.0	40.0
36	36.63842225416526	39.0	35.0	40.0	31.0	40.0
37	36.54688344411661	40.0	35.0	40.0	30.0	40.0
38	36.439098423768826	40.0	35.0	40.0	30.0	40.0
39	36.32229593632823	40.0	35.0	40.0	30.0	40.0
40	36.2047677385026	39.0	35.0	40.0	30.0	40.0
41	36.08109737937436	39.0	35.0	40.0	30.0	40.0
42	35.94467081894777	39.0	35.0	40.0	29.0	40.0
43	35.550158548569804	39.0	35.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	24.0
12	40.0
13	55.0
14	89.0
15	98.0
16	177.0
17	402.0
18	954.0
19	2272.0
20	5107.0
21	10290.0
22	20122.0
23	35772.0
24	60044.0
25	94730.0
26	140416.0
27	203169.0
28	284356.0
29	383402.0
30	495835.0
31	612579.0
32	746708.0
33	929410.0
34	1177132.0
35	1518224.0
36	2182622.0
37	3700392.0
38	9540848.0
39	3312534.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35221790841901	20.046226278501365	13.316893587171311	23.28466222590832
2	18.354694554685647	23.619180342821263	33.908115598613456	24.118009503879634
3	20.058635042270325	24.77580360984647	30.906491028648414	24.259070319234795
4	14.577583134199976	17.116944919151344	35.164822085666906	33.14064986098178
5	13.999823394106029	37.155872849098515	33.76769898625231	15.076604770543142
6	31.930443831508864	36.565500085749704	15.484483467433613	16.01957261530782
7	27.137608908604644	32.531524505599734	21.660481139503965	18.670385446291657
8	27.359356148960785	33.28363150111789	20.047986052533812	19.309026297387515
9	25.744083918595685	14.880417462394846	20.957549615218337	38.417949003791136
10	16.77694322065516	28.863012354477885	31.692556163698892	22.667488261168064
11	33.987596107034165	23.57943616054387	21.946601542457653	20.486366189964308
12	22.0903255872637	26.89465796238436	29.521705987796125	21.49331046255582
13	29.965267245648036	21.349083625169072	25.47480706709539	23.2108420620875
14	21.501641892740672	22.003220700724736	27.833536689363825	28.661600717170764
15	23.534389087766925	29.362301099379547	24.82747733171434	22.27583248113919
16	22.259307103115766	28.481125677169295	26.117152085406865	23.142415134308074
17	21.70307709845001	28.13396704053223	27.381683281997425	22.781272579020335
18	22.758489790207438	26.19159681188652	28.834089996886615	22.215823401019428
19	23.64483847670503	26.27895702278287	29.332027486727654	20.74417701378445
20	23.07376037864052	26.620325688607828	29.219496222260087	21.086417710491563
21	22.653366860171108	26.959467140422976	29.028592164276162	21.358573835129757
22	22.341835791556967	26.962067520801273	28.880099446087552	21.815997241554204
23	21.958157915679642	27.133940093992358	29.113803722503434	21.794098267824566
24	21.739525632483605	27.159739638289448	29.219091631355308	21.88164309787164
25	21.905930336359596	27.054695269593847	29.42111997518719	21.618254418859372
26	21.562986515652916	27.46070813464188	29.533580534448088	21.442724815257115
27	21.56739773493514	27.421254629325205	29.48127046572027	21.53007717001939
28	21.557082630896744	27.585879918833566	29.3882577663581	21.46877968391159
29	21.368366506252265	27.78920059672052	29.1857344860796	21.656698410947616
30	21.48050103594912	27.854512567339835	28.998251774484647	21.666734622226404
31	21.404858249023267	27.68597885112995	29.134088260680986	21.775074639165798
32	21.12324333896068	28.040891492097476	28.945136452014303	21.89072871692754
33	21.163160356187692	27.989873757424732	29.097451179429623	21.74951470695795
34	21.379938591742402	27.830205688516696	28.959776357860108	21.8300793618808
35	21.073360815661548	28.166463939126903	29.115308172178487	21.64486707303306
36	20.97314404135406	27.864847311713408	29.132387407654093	22.029621239278438
37	21.15046877159209	27.477783442050445	29.290692437300393	22.08105534905707
38	20.82515804381319	27.45988716863121	29.596351045694654	22.118603741860948
39	20.856130852397616	27.08228994052671	29.688731326263913	22.37284788081176
40	20.868154665596975	26.80708170780099	29.93035065775875	22.39441296884328
41	20.61072486427939	26.226690162792455	30.09350292212835	23.069082050799807
42	20.409462493519673	25.807439711830714	30.285844652647707	23.4972531420019
43	20.039175398170478	25.027290245737515	30.767252836398285	24.166281519693715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18262.0
1	15864.0
2	13466.0
3	27508.5
4	41551.0
5	41551.0
6	60355.5
7	79160.0
8	81962.5
9	84765.0
10	111054.0
11	137343.0
12	137343.0
13	216475.5
14	295608.0
15	451286.5
16	606965.0
17	591031.0
18	575097.0
19	575097.0
20	544960.5
21	514824.0
22	455901.5
23	396979.0
24	467555.5
25	538132.0
26	538132.0
27	621238.5
28	704345.0
29	749446.0
30	794547.0
31	839423.5
32	884300.0
33	884300.0
34	926716.0
35	969132.0
36	1012619.5
37	1056107.0
38	1108106.5
39	1160106.0
40	1160106.0
41	1205742.5
42	1251379.0
43	1316315.0
44	1381251.0
45	1456731.0
46	1532211.0
47	1532211.0
48	1606615.5
49	1681020.0
50	1711903.5
51	1742787.0
52	1749087.0
53	1755387.0
54	1755387.0
55	1681795.5
56	1608204.0
57	1531098.0
58	1453992.0
59	1346220.5
60	1238449.0
61	1238449.0
62	1089586.5
63	940724.0
64	801905.0
65	663086.0
66	569492.0
67	475898.0
68	475898.0
69	394563.5
70	313229.0
71	268055.0
72	222881.0
73	180205.5
74	137530.0
75	137530.0
76	110031.0
77	82532.0
78	65958.5
79	49385.0
80	38994.0
81	28603.0
82	28603.0
83	22255.0
84	15907.0
85	12472.0
86	9037.0
87	5876.5
88	2716.0
89	2716.0
90	1676.0
91	636.0
92	406.5
93	177.0
94	130.0
95	83.0
96	83.0
97	48.5
98	14.0
99	10.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2.5457814E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.99910304288866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.59805887747753	28.312591788764447
2	13.121609466562312	10.23461999350014
3	5.092921623959121	5.95858125606413
4	2.474213852331102	3.8596848390881267
5	1.4986450868702008	2.92229070837849
6	0.9900135943838648	2.316578530874216
7	0.6684948489418114	1.8249489648265398
8	0.4878161808086206	1.521951480107504
9	0.3761284638241819	1.3201805446238424
>10	2.3827953270264586	17.573996432104916
>50	0.18407162672273164	4.942644855323567
>100	0.11102152371221773	8.27888865074073
>500	0.008893088555177366	2.364681877236453
>1k	0.004754846845098408	3.4271745510266434
>5k	3.0085284616617234E-4	0.7851952336304462
>10k+	2.607391333417396E-4	4.355990293709858
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	261868	1.0286350587682038	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	259486	1.0192784030867694	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	191102	0.7506614668486461	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93782	0.3683819828363897	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31572	0.12401693248289111	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7496469256944055E-5
2	1.217700781378951E-4	0.0	0.0	3.1424536293650353E-5	2.7496469256944055E-5
3	1.5319461443154546E-4	0.0	0.0	4.3208737403769235E-5	2.7496469256944055E-5
4	1.5712268146825175E-4	0.0	0.0	5.892100555059441E-5	2.7496469256944055E-5
5	4.595838432946364E-4	0.0	0.0	1.2569814517460141E-4	2.7496469256944055E-5
6	6.402749269831259E-4	0.0	0.0	1.6105074850495806E-4	5.892100555059441E-5
7	6.913397984603078E-4	0.0	0.0	1.7676301665178323E-4	5.892100555059441E-5
8	8.131098765982028E-4	0.0	0.0	2.0818755294543356E-4	5.892100555059441E-5
9	8.484624799285595E-4	0.0	0.0	2.553243573859091E-4	7.070520666071329E-5
10	0.0013983918650674406	0.0	0.0	3.9673477070733567E-4	7.463327369741959E-5
11	0.0015162338761686295	0.0	0.0	4.5172770922122376E-4	8.248940777083217E-5
12	0.0015830110157926363	0.0	0.0	4.752961114414615E-4	9.820167591765734E-5
13	0.0016419320213432308	0.0	0.0	5.106487147718182E-4	1.1391394406448252E-4
14	0.0018265511720684266	0.0	0.0	5.852819884692378E-4	1.3355427924801398E-4
15	0.0019601054513164406	0.0	0.0	6.756275303134825E-4	1.7283494961507694E-4
16	0.002097587797601161	0.0	0.0	0.001001657094360105	1.846191507251958E-4
17	0.0021093719987112796	0.0	0.0	0.001484809339874979	1.846191507251958E-4
18	0.002152580736115049	0.0	0.0	0.002022954523903741	1.885472177619021E-4
19	0.002235070143885881	0.0	0.0	0.0027889275960614685	1.885472177619021E-4
20	0.0026003803782995664	0.0	0.0	0.005000429337727112	2.1997175405555244E-4
21	0.003731663684870979	0.0	0.0	0.01046044251874886	2.1997175405555244E-4
22	0.0037748724222747485	0.0	0.0	0.015892959230513665	2.2782788812896504E-4
23	0.00379451275745828	0.0	0.0	0.02025311364125765	2.474682233124965E-4
24	0.003837721494862049	0.0	0.0	0.025123916766773453	2.5139629034920283E-4
25	0.0038966425004126433	3.928067036706294E-6	0.0	0.02826637039613849	2.5139629034920283E-4
26	0.004206959796312441	3.928067036706294E-6	0.0	0.036020374726596714	2.592524244226154E-4
27	0.004332657941487042	3.928067036706294E-6	0.0	0.08818117690701958	2.631804914593217E-4
28	0.0043876508800009305	3.928067036706294E-6	0.0	0.1910336841961372	2.631804914593217E-4
29	0.0043994350811110495	3.928067036706294E-6	0.0	0.3636368778560484	2.631804914593217E-4
30	0.0044112192822211675	3.928067036706294E-6	0.0	0.5331918914954756	2.6710855849602797E-4
31	0.004446571885551524	3.928067036706294E-6	0.0	0.6367553789182371	2.749646925694406E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	97185	0.0	16.06246	1
GTATCAA	152865	0.0	12.7690115	1
TACACCG	1515	0.0	12.21122	5
CTACTCG	5640	0.0	11.939716	14
TATCTCG	4770	0.0	11.790357	36
TATACAC	14430	0.0	11.5	37
TACTCGG	5855	0.0	11.4380865	15
AACGCAG	164265	0.0	11.391806	6
TATACAG	4800	0.0	11.331249	5
ACGGACT	4950	0.0	11.286868	27
TATACTG	3290	0.0	11.077508	5
CTGTCTC	102720	0.0	11.004187	37
TAGTCCC	6610	0.0	10.831315	5
TACACAG	6745	0.0	10.751669	5
TGTCTCT	67375	0.0	10.686752	37
TTAGGCA	6805	0.0	10.656869	4
ACGAGAC	5740	0.0	10.539199	22
TAGCGCA	4375	0.0	10.486857	4
CGAGACG	5840	0.0	10.39041	23
TGAACCG	5565	0.0	10.371967	14
>>END_MODULE