FastQCFastQC Report
Thu 26 May 2016
SRR1041758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58356596
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2571050.4405757320046564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2243000.3843610069374163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1795770.30772356907178067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT784060.13435670579551967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA919250.022.5743271
GTATCAA1688750.016.389551
CTACTCG135300.012.606814
AACGCAG2184500.012.3804766
TGAACCG136600.012.35139114
AACCGGC139200.012.1206916
TAGCGCA101400.012.0961544
TACTCGG139800.012.05543615
TATACAG155350.011.9919545
GAACCGG143550.011.84360915
TATCAAC2335050.011.7819122
ATCAACG2381850.011.494493
GCCTAAG175800.011.4809441
TCAACGC2392800.011.45348554
ACGCAGA2361500.011.445487
CAACGCA2396950.011.4421455
CGCAGAG2374700.011.3717328
TTAGGCA182150.011.2838334
TCGGAAA147900.011.28262428
TATACTG91150.011.24410255
Produced by FastQC (version 0.11.5)