##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041758_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58356596
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29852462607654	33.0	31.0	34.0	31.0	34.0
2	32.427757009678906	34.0	31.0	34.0	31.0	34.0
3	32.50697514981854	34.0	31.0	34.0	30.0	34.0
4	36.024900698457465	37.0	35.0	37.0	35.0	37.0
5	35.985684480294225	37.0	35.0	37.0	35.0	37.0
6	36.048433051167	37.0	35.0	37.0	35.0	37.0
7	35.97934493985907	37.0	35.0	37.0	35.0	37.0
8	35.983656928858565	37.0	35.0	37.0	35.0	37.0
9	37.72294029967067	39.0	38.0	39.0	35.0	39.0
10	37.633889406434875	39.0	37.0	39.0	35.0	39.0
11	37.71629758870788	39.0	37.0	39.0	35.0	39.0
12	37.63366207994723	39.0	37.0	39.0	35.0	39.0
13	37.64688634340495	39.0	37.0	39.0	35.0	39.0
14	38.49977056578146	40.0	38.0	40.0	35.0	40.0
15	38.48080994991552	40.0	38.0	40.0	35.0	40.0
16	38.45553644013095	40.0	38.0	40.0	35.0	40.0
17	38.449030971580314	40.0	38.0	40.0	35.0	40.0
18	38.414886485154135	40.0	38.0	40.0	35.0	40.0
19	38.421855106147724	40.0	38.0	40.0	35.0	40.0
20	38.36435087817665	40.0	38.0	40.0	34.0	40.0
21	38.30095816418079	40.0	38.0	40.0	34.0	40.0
22	38.24886218517612	40.0	38.0	40.0	34.0	40.0
23	38.17048840545806	40.0	38.0	40.0	34.0	40.0
24	38.11385590413807	40.0	38.0	40.0	34.0	40.0
25	38.090692558558416	40.0	38.0	40.0	34.0	40.0
26	37.96401465911411	40.0	38.0	40.0	34.0	40.0
27	37.87849013674478	40.0	38.0	40.0	33.0	40.0
28	37.75299386893643	40.0	38.0	40.0	33.0	40.0
29	37.675805816363926	40.0	38.0	40.0	33.0	40.0
30	37.63122134471312	40.0	37.0	40.0	33.0	40.0
31	37.56319335349855	40.0	37.0	40.0	33.0	40.0
32	37.4579168051543	40.0	37.0	40.0	33.0	40.0
33	37.459433343233385	40.0	37.0	40.0	33.0	40.0
34	37.371997914340305	40.0	37.0	40.0	33.0	40.0
35	37.3913419487319	40.0	37.0	40.0	33.0	40.0
36	37.439877130598916	40.0	37.0	40.0	33.0	40.0
37	37.41092360836126	40.0	37.0	40.0	33.0	40.0
38	37.35941712570075	40.0	37.0	40.0	33.0	40.0
39	37.313307616503195	40.0	37.0	40.0	33.0	40.0
40	37.277097433853065	40.0	37.0	40.0	32.0	40.0
41	37.2140285050211	40.0	37.0	40.0	32.0	40.0
42	37.15207641652025	40.0	37.0	40.0	32.0	40.0
43	36.82401091386482	40.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	21.0
12	37.0
13	81.0
14	98.0
15	149.0
16	250.0
17	572.0
18	1330.0
19	3148.0
20	6874.0
21	14456.0
22	29095.0
23	53297.0
24	92378.0
25	146673.0
26	223731.0
27	328115.0
28	465218.0
29	635243.0
30	843565.0
31	1076061.0
32	1349129.0
33	1701111.0
34	2206051.0
35	2934032.0
36	4233141.0
37	7288024.0
38	2.3788864E7
39	1.0935845E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.102563761601175	19.695106959288715	12.54673250646765	24.65559677264246
2	17.933405505694676	22.263532986056966	35.17988437845141	24.623177129796947
3	19.053390297131106	23.85382279665524	31.357697765647607	25.735089140566046
4	13.738313317658212	16.627111012438082	36.84269898127711	32.7918766886266
5	13.802753334001865	36.55961530038524	35.08583845431971	14.551792911293182
6	32.01946700249617	37.277522150195324	15.929064813855831	14.77394603345267
7	27.925389959345814	32.307823437816694	21.499285187916033	18.267501414921462
8	26.65534672378766	34.06937752160869	20.220535138821326	19.054740615782322
9	25.780506457230643	15.05657732332434	20.663446510828013	38.499469708617
10	16.215279246239792	28.279277975706464	32.674169343256416	22.83127343479733
11	34.90644485158113	23.05804814249275	22.363533678352315	19.671973327573802
12	22.10870558659727	26.106029213904115	29.69484717717257	22.090418022326045
13	29.432705430590918	21.035800306104218	25.37463460000306	24.156859663301812
14	21.383385693024316	21.551753978247806	27.13536272746272	29.929497601265158
15	23.87070520700008	28.936967125361456	23.990592254558507	23.201735413079955
16	23.243828341187	28.35883367837288	25.151107168759467	23.246230811680654
17	22.548188040303106	27.662280027436832	26.583733568010032	23.205798364250033
18	23.37745333877939	26.242012128329073	27.68597743432465	22.694557098566886
19	24.57635808641066	26.15245926955712	27.458251677325386	21.81293096670683
20	23.993421412037126	26.481868476358695	27.51735553595347	22.007354575650712
21	23.883214504149624	26.521564074779143	27.216774261473375	22.378447159597865
22	23.39493208274177	26.493258791174178	27.13662393879177	22.975185187292283
23	23.013521899049767	26.477723272275856	27.393007981479933	23.115746847194448
24	22.719450599894483	26.52412076948422	27.570232163644366	23.18619646697693
25	23.028615308541987	26.379592120143542	27.733289995187523	22.858502576126956
26	22.72638383499956	26.59776968485276	27.786785233326494	22.889061246821182
27	22.868338653611666	26.612714010940596	27.716810281394753	22.80213705405298
28	22.781666360388808	26.725052640150565	27.66117818112626	22.832102818334366
29	22.629282557879147	26.902994821699334	27.58712656920565	22.880596051215875
30	22.758704088908818	26.984313821183125	27.395412165575937	22.861569924332116
31	22.710246841676646	26.732234347596282	27.434717062660745	23.12280174806632
32	22.353877529114275	27.05111860876875	27.55253407858128	23.042469783535697
33	22.53962345576154	26.901408025923924	27.58696206338012	22.97200645493442
34	22.704430875303284	26.69387707261061	27.743355695387027	22.858336356699077
35	22.52471854252774	27.09384899694972	27.705976887342775	22.675455573179764
36	22.335778461101466	26.64277573695354	27.822964519726273	23.198481282218722
37	22.598086427111	26.35097324730867	27.75574846757683	23.295191858003506
38	22.06312890491419	26.291865961475892	28.258303482951607	23.386701650658306
39	22.09621685267592	25.777881218431588	28.36894050502877	23.756961423863725
40	22.145171044589375	25.42921968923616	28.81191699392473	23.61369227224974
41	21.89281054021725	24.86713584185068	29.216935477182393	24.023118140749677
42	21.69465641895905	24.372819826571103	29.49373195105486	24.43879180341499
43	21.251664507641944	23.549690938107492	30.092169872279733	25.106474681970823
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21962.0
1	17704.0
2	13446.0
3	27656.0
4	41866.0
5	41866.0
6	59635.0
7	77404.0
8	78770.0
9	80136.0
10	103051.5
11	125967.0
12	125967.0
13	191432.0
14	256897.0
15	385442.5
16	513988.0
17	535226.5
18	556465.0
19	556465.0
20	551752.5
21	547040.0
22	574035.0
23	601030.0
24	813759.0
25	1026488.0
26	1026488.0
27	1319113.0
28	1611738.0
29	1836557.0
30	2061376.0
31	2273404.0
32	2485432.0
33	2485432.0
34	2727249.0
35	2969066.0
36	3089445.5
37	3209825.0
38	3304203.0
39	3398581.0
40	3398581.0
41	3331911.5
42	3265242.0
43	3274996.5
44	3284751.0
45	3385418.5
46	3486086.0
47	3486086.0
48	3594556.5
49	3703027.0
50	3775485.0
51	3847943.0
52	3888022.0
53	3928101.0
54	3928101.0
55	3788661.0
56	3649221.0
57	3523881.0
58	3398541.0
59	3156777.5
60	2915014.0
61	2915014.0
62	2578342.0
63	2241670.0
64	1909598.5
65	1577527.0
66	1364639.5
67	1151752.0
68	1151752.0
69	958998.0
70	766244.0
71	673996.0
72	581748.0
73	475053.0
74	368358.0
75	368358.0
76	303178.5
77	237999.0
78	196551.0
79	155103.0
80	124381.5
81	93660.0
82	93660.0
83	75542.0
84	57424.0
85	45394.5
86	33365.0
87	22458.0
88	11551.0
89	11551.0
90	6996.5
91	2442.0
92	1593.0
93	744.0
94	529.5
95	315.0
96	315.0
97	175.0
98	35.0
99	30.5
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5.8356596E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.42997922404267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.875926097698	23.713967045804406
2	14.423632307485324	9.932107213639005
3	5.956363425765051	6.152324069998149
4	3.1587213089772463	4.350188361705098
5	1.771042475725607	3.048847782206487
6	1.1340799426677566	2.342780931867262
7	0.812424294767067	1.958022611295634
8	0.5601565903043284	1.5428943813110885
9	0.4289366769946466	1.3291452789620022
>10	2.488214239206739	15.91465204425868
>50	0.21704723539513554	5.1934253544888165
>100	0.14668683410580724	9.902234977832098
>500	0.015171221378497133	3.5901555851019165
>1k	0.010685305174114297	7.092425223499098
>5k	7.226593791587058E-4	1.7006971895477083
>10k+	1.8938497540140248E-4	2.2361319484825937
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	257105	0.4405757320046564	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	224300	0.3843610069374163	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	179577	0.30772356907178067	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78406	0.13435670579551967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.140807047758577E-6	0.0	0.0	0.0	2.056322819103431E-5
2	4.284005873132148E-5	0.0	0.0	1.7136023492528592E-6	2.056322819103431E-5
3	5.826247987459721E-5	0.0	0.0	3.4272046985057184E-6	2.056322819103431E-5
4	6.168968457310293E-5	0.0	0.0	8.568011746264295E-6	2.2276830540287167E-5
5	2.0049147486258452E-4	0.0	0.0	1.884962584178145E-5	2.3990432889540026E-5
6	2.776035805789632E-4	0.0	0.0	2.2276830540287167E-5	4.112645638206862E-5
7	2.9988041111925033E-4	0.0	0.0	2.7417637588045747E-5	4.284005873132148E-5
8	3.684245050893647E-4	0.0	0.0	3.255844463580432E-5	4.798086577908005E-5
9	3.975557450266633E-4	0.0	0.0	6.511688927160864E-5	6.168968457310293E-5
10	6.357464715728107E-4	0.0	0.0	1.4736980203574587E-4	6.340328692235579E-5
11	6.871545420503965E-4	0.0	0.0	1.884962584178145E-4	6.68304916208615E-5
12	7.077177702414308E-4	0.0	0.0	2.1248669130735453E-4	8.910732216114867E-5
13	7.488442266234994E-4	0.0	0.0	2.261955101013774E-4	1.1481135739994156E-4
14	8.739371981189582E-4	0.0	0.0	2.55326750038676E-4	1.3023377854321728E-4
15	9.476220991368311E-4	0.0	0.0	3.050212181670089E-4	1.4736980203574587E-4
16	0.001009311783709934	0.0	0.0	6.100424363340178E-4	1.627922231790216E-4
17	0.0010230206025039568	0.0	0.0	0.0011104143223158527	1.627922231790216E-4
18	0.0010384430236472326	0.0	0.0	0.0020374731932616495	1.8335545137005592E-4
19	0.0010641470588860255	0.0	0.0	0.0029114103913806077	1.8506905371930879E-4
20	0.001262924931399357	0.0	0.0	0.005965049777749203	2.142002936566074E-4
21	0.0017941416596677436	0.0	0.0	0.011042453538585423	2.2276830540287167E-4
22	0.0018044232737632606	0.0	0.0	0.01733308776269267	2.3133631714913598E-4
23	0.0018404089230975706	0.0	0.0	0.020926511889075915	2.6560836413419314E-4
24	0.001860972151288605	0.0	0.0	0.026368912950302997	2.758899782297103E-4
25	0.0018883897888766507	0.0	0.0	0.029239196885301535	2.810307852774689E-4
26	0.002006628350975098	0.0	0.0	0.0371320493059602	2.9988041111925033E-4
27	0.0020511820120556724	0.0	0.0	0.0826213372692266	3.050212181670089E-4
28	0.0020854540590407294	0.0	0.0	0.18752807309048664	3.0844842286551464E-4
29	0.002092308468437741	0.0	0.0	0.33180996369287885	3.0844842286551464E-4
30	0.002100876480184005	0.0	0.0	0.47289769951626376	3.1187562756402037E-4
31	0.0021214397083750398	0.0	0.0	0.5683213599367585	3.1701643461177895E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	91925	0.0	22.574327	1
GTATCAA	168875	0.0	16.38955	1
CTACTCG	13530	0.0	12.6068	14
AACGCAG	218450	0.0	12.380476	6
TGAACCG	13660	0.0	12.351391	14
AACCGGC	13920	0.0	12.12069	16
TAGCGCA	10140	0.0	12.096154	4
TACTCGG	13980	0.0	12.055436	15
TATACAG	15535	0.0	11.991954	5
GAACCGG	14355	0.0	11.843609	15
TATCAAC	233505	0.0	11.781912	2
ATCAACG	238185	0.0	11.49449	3
GCCTAAG	17580	0.0	11.480944	1
TCAACGC	239280	0.0	11.4534855	4
ACGCAGA	236150	0.0	11.44548	7
CAACGCA	239695	0.0	11.442145	5
CGCAGAG	237470	0.0	11.371732	8
TTAGGCA	18215	0.0	11.283833	4
TCGGAAA	14790	0.0	11.282624	28
TATACTG	9115	0.0	11.2441025	5
>>END_MODULE