FastQCFastQC Report
Thu 26 May 2016
SRR1041757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28805189
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2843190.9870409112747012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2653840.9213062271523371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2014620.6993948208428696No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT924580.3209768906567494No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT307660.10680714505987099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1024500.016.8964861
CTACTCG85300.014.14067914
TACTCGG87400.013.80091615
GTATCAA1658750.013.3701881
TAGCGCA64450.012.6299464
CGTATCG12500.012.58000110
GCCTAAG102300.012.5141741
GAACCGG92900.012.48600715
AACCGGC92300.012.46695716
TGAACCG94300.012.24178214
CGGAAAC95300.012.07450229
TTAGGCA109250.011.9720834
TAGTCCC106900.011.9583735
ATAGCGC67200.011.8928583
AACGCAG1800200.011.8880136
TATACAG63300.011.8072685
TATACAC166950.011.80143837
CCTGTAG110700.011.7818431
TCGGAAA95350.011.68012628
ACTCGGG105050.011.64064816
Produced by FastQC (version 0.11.5)