##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041757_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28805189
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.174561013989525	33.0	31.0	34.0	30.0	34.0
2	32.256015574138395	34.0	31.0	34.0	30.0	34.0
3	32.35695315173943	34.0	31.0	34.0	30.0	34.0
4	35.903006642310174	37.0	35.0	37.0	35.0	37.0
5	35.876098712631254	37.0	35.0	37.0	35.0	37.0
6	35.956581399274974	37.0	35.0	37.0	35.0	37.0
7	35.89160588392598	37.0	35.0	37.0	35.0	37.0
8	35.892710441858235	37.0	35.0	37.0	35.0	37.0
9	37.601322143729035	39.0	37.0	39.0	35.0	39.0
10	37.51219538951819	39.0	37.0	39.0	35.0	39.0
11	37.591305024938386	39.0	37.0	39.0	35.0	39.0
12	37.51334868866856	39.0	37.0	39.0	35.0	39.0
13	37.52770721275254	39.0	37.0	39.0	35.0	39.0
14	38.34094673011866	40.0	38.0	40.0	34.0	40.0
15	38.316992782099085	40.0	38.0	40.0	34.0	40.0
16	38.29528342966262	40.0	38.0	40.0	34.0	40.0
17	38.32536287125212	40.0	38.0	40.0	34.0	40.0
18	38.3049891462264	40.0	38.0	40.0	34.0	40.0
19	38.32616734436285	40.0	38.0	40.0	34.0	40.0
20	38.26575295166437	40.0	38.0	40.0	34.0	40.0
21	38.20924306381048	40.0	38.0	40.0	34.0	40.0
22	38.15206284534359	40.0	38.0	40.0	34.0	40.0
23	38.07396580525821	40.0	38.0	40.0	34.0	40.0
24	38.018497570003795	40.0	38.0	40.0	34.0	40.0
25	37.9813746405205	40.0	38.0	40.0	34.0	40.0
26	37.82723276004195	40.0	38.0	40.0	33.0	40.0
27	37.73305733213554	40.0	37.0	40.0	33.0	40.0
28	37.576778024265	40.0	37.0	40.0	33.0	40.0
29	37.50058015588789	40.0	37.0	40.0	33.0	40.0
30	37.37183963625443	40.0	37.0	40.0	33.0	40.0
31	37.26528827844178	40.0	36.0	40.0	32.0	40.0
32	37.08815175626864	40.0	36.0	40.0	32.0	40.0
33	37.0946897102463	40.0	36.0	40.0	32.0	40.0
34	36.97508983537654	40.0	36.0	40.0	31.0	40.0
35	37.01006447137007	40.0	36.0	40.0	32.0	40.0
36	36.977054585547066	40.0	36.0	40.0	32.0	40.0
37	36.8932904068083	40.0	36.0	40.0	31.0	40.0
38	36.7843012590544	40.0	35.0	40.0	31.0	40.0
39	36.68561511608203	40.0	35.0	40.0	31.0	40.0
40	36.58401227639923	40.0	35.0	40.0	31.0	40.0
41	36.473874099558934	40.0	35.0	40.0	30.0	40.0
42	36.346572695634805	40.0	35.0	40.0	30.0	40.0
43	35.992056708949214	39.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	20.0
11	28.0
12	43.0
13	82.0
14	97.0
15	132.0
16	203.0
17	373.0
18	821.0
19	1959.0
20	4448.0
21	9429.0
22	18276.0
23	32786.0
24	55495.0
25	88475.0
26	134245.0
27	198094.0
28	281627.0
29	385382.0
30	504826.0
31	629187.0
32	769996.0
33	958705.0
34	1219157.0
35	1585435.0
36	2277669.0
37	3953452.0
38	1.090368E7
39	4791061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73050286182812	19.962882382059703	12.827102089140954	23.47951266697122
2	17.743969671575492	23.268505545997286	34.70981218002076	24.277712602406464
3	19.171594395718078	25.106507719841726	31.302280294012302	24.41961759042789
4	13.491833016613777	16.99315703153345	36.722949465806316	32.79206048604645
5	13.26470032881923	37.48477748227932	34.59114953212076	14.659372656780691
6	31.06998187028039	37.91551237521823	15.5282056993273	15.486300055174087
7	26.896143608014516	32.96687621108822	22.02543090413328	18.111549276763988
8	27.16764677364207	33.69572058700952	20.020125540575346	19.116507098773074
9	25.78261159820892	14.80959906216897	21.153622703187263	38.254166636434846
10	16.542071638550958	28.486551502925394	32.155782765389944	22.815594093133708
11	33.776084579760955	23.818559218618564	22.405601990669112	19.999754210951366
12	22.06962780212968	27.119707494368463	29.72448123843242	21.086183465069436
13	29.503166946760878	21.357082572865604	25.89970161278928	23.24004886758424
14	21.195087454555498	22.105662976208905	28.20254711746554	28.496702451770062
15	23.460311265445956	29.343619304146902	24.979985376940245	22.216084053466894
16	22.155789361423736	28.75221544284955	26.049962734144877	23.042032461581837
17	21.604885147603092	28.19915189586154	27.494015054023773	22.701947902511595
18	22.509569369602122	26.2472882923976	29.0364246525166	22.20671768548368
19	23.83741346047061	26.0961731582459	29.306028854731693	20.760384526551796
20	23.098987477568713	26.719921886296248	29.409045016160107	20.77204561997493
21	22.781721723818578	26.714648530860185	29.04920360008747	21.45442614523376
22	22.158549280825756	26.840146752725698	29.054844944777138	21.94645902167141
23	21.924403967632358	27.10406795109034	29.24097113197209	21.73055694930521
24	21.67925716439493	27.186629464573205	29.239953954129582	21.89415941690228
25	21.995491853915627	27.12335961413063	29.547162492146818	21.333986039806927
26	21.663530831198504	27.152760566854813	29.7864214673266	21.397287134620086
27	21.557633244482442	27.27151694786658	29.58334694488552	21.587502862765458
28	21.438363067154324	27.22630981522114	29.751066726206865	21.58426039141767
29	21.16370074850056	27.77470059300774	29.33319757075713	21.728401087734575
30	21.514821513582152	27.657614050024108	29.172969495183665	21.65459494121007
31	21.33825263219068	27.365388923502636	29.340685804908272	21.955672639398408
32	21.03074900845122	27.766806182038938	29.327601356824978	21.87484345268486
33	21.045472050192068	27.958153650718977	29.247445659877464	21.7489286392115
34	21.555585002410503	27.521267088370777	29.386163722098818	21.5369841871199
35	21.069589232689985	28.185841793990658	29.266518612323633	21.47805036099572
36	21.045121418922125	27.779387248596077	29.165942983397887	22.00954834908391
37	21.528048297131463	27.362129094171195	29.11388986199674	21.995932746700603
38	20.86052620588603	27.58021132928515	29.495439172435216	22.063823292393604
39	20.74066238551672	27.164275853215198	29.679638623443854	22.41542313782423
40	20.784251754085002	26.827760095585557	30.0278987928182	22.360089357511246
41	20.59233147194417	26.27709889353616	30.214399912460216	22.91616972205945
42	20.55539715431133	25.892397373264934	30.31267734434931	23.239528128074426
43	20.22516151517006	25.00187032273942	30.80332505369085	23.969643108399673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8764.0
1	8708.5
2	8653.0
3	23055.0
4	37457.0
5	37457.0
6	56546.5
7	75636.0
8	77716.5
9	79797.0
10	105112.5
11	130428.0
12	130428.0
13	207625.0
14	284822.0
15	440727.0
16	596632.0
17	594269.0
18	591906.0
19	591906.0
20	544529.0
21	497152.0
22	454621.0
23	412090.0
24	512686.5
25	613283.0
26	613283.0
27	718788.5
28	824294.0
29	931311.0
30	1038328.0
31	1120121.5
32	1201915.0
33	1201915.0
34	1290111.5
35	1378308.0
36	1438789.5
37	1499271.0
38	1544349.5
39	1589428.0
40	1589428.0
41	1566824.5
42	1544221.0
43	1548564.0
44	1552907.0
45	1594404.5
46	1635902.0
47	1635902.0
48	1673670.5
49	1711439.0
50	1728711.5
51	1745984.0
52	1757659.0
53	1769334.0
54	1769334.0
55	1719902.0
56	1670470.0
57	1616276.0
58	1562082.0
59	1457364.0
60	1352646.0
61	1352646.0
62	1206760.5
63	1060875.0
64	916312.0
65	771749.0
66	660283.5
67	548818.0
68	548818.0
69	450737.5
70	352657.0
71	308390.0
72	264123.0
73	211850.5
74	159578.0
75	159578.0
76	129670.5
77	99763.0
78	82211.5
79	64660.0
80	49843.5
81	35027.0
82	35027.0
83	27366.5
84	19706.0
85	15236.5
86	10767.0
87	6943.5
88	3120.0
89	3120.0
90	1975.5
91	831.0
92	532.5
93	234.0
94	166.5
95	99.0
96	99.0
97	58.5
98	18.0
99	16.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2.8805189E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.226772029618964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.08933989251396	22.90979950452144
2	13.935513335368826	8.981932227492923
3	5.307611514239918	5.131415588735716
4	2.615072867913594	3.3710142862037302
5	1.5229297831162154	2.4539555468801657
6	1.0212027588158827	1.9746041102626444
7	0.7132875395741283	1.609086845059627
8	0.50303935479369	1.2969067687090285
9	0.37594348008938155	1.0903900345975885
>10	2.4592208299437304	15.10397529364349
>50	0.24251934107124878	5.440366104380409
>100	0.17828879545718934	11.500857707519417
>500	0.020779285851049482	4.622290740672917
>1k	0.01407750039254864	8.433592194264477
>5k	8.506784205212928E-4	1.7540289998948415
>10k+	3.2304243816443063E-4	4.3257840471617
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	284319	0.9870409112747012	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	265384	0.9213062271523371	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	201462	0.6993948208428696	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92458	0.3209768906567494	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30766	0.10680714505987099	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4715967321026777E-6	0.0	0.0	0.0	2.0829580392616068E-5
2	1.0761949869518301E-4	0.0	0.0	3.4715967321026777E-6	2.0829580392616068E-5
3	1.2844907908779907E-4	0.0	0.0	3.4715967321026777E-6	2.0829580392616068E-5
4	1.3192067581990175E-4	0.0	0.0	1.0414790196308034E-5	2.777277385682142E-5
5	3.714608503349865E-4	0.0	0.0	1.388638692841071E-5	2.777277385682142E-5
6	5.693418640648391E-4	0.0	0.0	2.0829580392616068E-5	4.513075751733481E-5
7	6.179442183142766E-4	0.0	0.0	2.4301177124718745E-5	5.2073950981540166E-5
8	8.019388451157186E-4	0.0	0.0	3.4715967321026775E-5	5.554554771364284E-5
9	8.713707797577721E-4	0.0	0.0	6.943193464205355E-5	7.63751281062589E-5
10	0.0014927865948041515	0.0	0.0	1.7705143333723657E-4	7.63751281062589E-5
11	0.001583048109838821	0.0	0.0	3.089721091571383E-4	7.98467248383616E-5
12	0.0016490084477487719	0.0	0.0	3.610460601386785E-4	9.026151503466963E-5
13	0.0017844007203007763	0.0	0.0	3.9576202745970527E-4	9.37331117667723E-5
14	0.0020343556850121692	0.0	0.0	4.4783597844124545E-4	1.249774823556964E-4
15	0.0022218219085457136	0.0	0.0	4.999099294227856E-4	1.4233546601620978E-4
16	0.0023294414072408966	0.0	0.0	0.0010727233902197274	1.562218529446205E-4
17	0.0023572141810977183	0.0	0.0	0.0017080255921945176	1.562218529446205E-4
18	0.002381515358222437	0.0	0.0	0.0023537425843656156	1.7010823987303122E-4
19	0.002457890486328696	0.0	0.0	0.0033014884922296464	1.735798366051339E-4
20	0.003006402770000919	0.0	0.0	0.00552331040077536	1.735798366051339E-4
21	0.004436700623627222	0.0	0.0	0.010959830883248154	1.8052303006933925E-4
22	0.004492246171340865	0.0	0.0	0.016486612880755615	1.8052303006933925E-4
23	0.0045373769288582	0.0	0.0	0.020583097024636777	2.4648336797929014E-4
24	0.004599865670036048	0.0	0.0	0.025512764384222578	2.499549647113928E-4
25	0.004662354411213896	0.0	0.0	0.028692746990828633	2.534265614434955E-4
26	0.005065059632137807	0.0	0.0	0.03528878078182372	2.5689815817559814E-4
27	0.005221281485082428	0.0	0.0	0.08197481363514053	2.5689815817559814E-4
28	0.005315014596849199	0.0	0.0	0.19053858664145548	2.638413516398035E-4
29	0.005349730564170227	0.0	0.0	0.36553136311655515	2.638413516398035E-4
30	0.005363616951098637	0.0	0.0	0.540058945629553	2.638413516398035E-4
31	0.005408747708615972	0.0	0.0	0.6463106352122876	2.673129483719062E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	102450	0.0	16.896486	1
CTACTCG	8530	0.0	14.140679	14
TACTCGG	8740	0.0	13.800916	15
GTATCAA	165875	0.0	13.370188	1
TAGCGCA	6445	0.0	12.629946	4
CGTATCG	1250	0.0	12.580001	10
GCCTAAG	10230	0.0	12.514174	1
GAACCGG	9290	0.0	12.486007	15
AACCGGC	9230	0.0	12.466957	16
TGAACCG	9430	0.0	12.241782	14
CGGAAAC	9530	0.0	12.074502	29
TTAGGCA	10925	0.0	11.972083	4
TAGTCCC	10690	0.0	11.958373	5
ATAGCGC	6720	0.0	11.892858	3
AACGCAG	180020	0.0	11.888013	6
TATACAG	6330	0.0	11.807268	5
TATACAC	16695	0.0	11.801438	37
CCTGTAG	11070	0.0	11.781843	1
TCGGAAA	9535	0.0	11.680126	28
ACTCGGG	10505	0.0	11.640648	16
>>END_MODULE