FastQCFastQC Report
Thu 26 May 2016
SRR1041756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22745894
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT996230.43798234529713365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT974490.4284245763213352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT746120.328024038096722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335460.1474815630460601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA480350.017.7393571
TATACCG12250.013.5918365
GTATCAA777100.013.4363651
TAGCGCA48600.012.4475314
TTAGGCA86200.012.2975644
TGAACCG65750.012.07072214
TAGTCCC76700.011.9393735
CTACTCG72500.011.91655214
TATACTG30900.011.9142395
TACCTAT30300.011.90593932
ATACCGT12450.011.73895556
CGTATAC6150.011.7317073
AACGCAG869650.011.70649056
ACCTATC31150.011.69983933
TATACAC106800.011.67509337
TACTCGG74850.011.5918515
CGGAAAC68350.011.44915829
GCCTAAG82750.011.4465251
ATAGCGC53200.011.4407883
CGTATCG6350.011.36220410
Produced by FastQC (version 0.11.5)