FastQCFastQC Report
Thu 26 May 2016
SRR1041755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28155783
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2677090.950813550452495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2300980.8172317566163939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1967490.6987871727808103No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT757760.2691312118721756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA815650.019.6420041
GTATCAA1391150.014.763831
TATACCG13950.013.5268825
TTAGGCA91150.012.7054314
TAGCGCA56350.012.5740914
TATACAG61800.012.5428815
TATACTG39450.012.1926515
AACGCAG1648850.012.1691496
CTACTCG81900.012.03968314
CTTAGGC96700.011.899693
TACTCGG83000.011.79096515
TAATACT58650.011.4185864
TAGTCCC91200.011.400225
GCCTAAG82000.011.3030491
ATAGCGC61900.011.2673673
TATCAAC1833150.011.1737732
TGAACCG68450.011.16216414
TAGGCAA100450.011.1607775
TAGACAG42100.010.9418065
TACACAG79200.010.9084615
Produced by FastQC (version 0.11.5)