##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1041755_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28155783 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17831963685755 33.0 31.0 34.0 30.0 34.0 2 32.258834996703875 34.0 31.0 34.0 30.0 34.0 3 32.356181783330264 34.0 31.0 34.0 30.0 34.0 4 35.90520380839702 37.0 35.0 37.0 35.0 37.0 5 35.86911797125301 37.0 35.0 37.0 35.0 37.0 6 35.95028587910342 37.0 35.0 37.0 35.0 37.0 7 35.882283081951584 37.0 35.0 37.0 35.0 37.0 8 35.880492366346196 37.0 35.0 37.0 35.0 37.0 9 37.59273155358528 39.0 37.0 39.0 35.0 39.0 10 37.48668403219332 39.0 37.0 39.0 34.0 39.0 11 37.57584429458062 39.0 37.0 39.0 35.0 39.0 12 37.485746036613506 39.0 37.0 39.0 35.0 39.0 13 37.504251364630846 39.0 37.0 39.0 35.0 39.0 14 38.32945441439153 40.0 38.0 40.0 34.0 40.0 15 38.302362076025375 40.0 38.0 40.0 34.0 40.0 16 38.275111049122664 40.0 38.0 40.0 34.0 40.0 17 38.28883451758383 40.0 38.0 40.0 34.0 40.0 18 38.2634750026309 40.0 38.0 40.0 34.0 40.0 19 38.27533412940425 40.0 38.0 40.0 34.0 40.0 20 38.213000860249565 40.0 38.0 40.0 34.0 40.0 21 38.1537236950576 40.0 38.0 40.0 34.0 40.0 22 38.098136180407415 40.0 38.0 40.0 34.0 40.0 23 38.019667824546026 40.0 38.0 40.0 34.0 40.0 24 37.9607441568931 40.0 38.0 40.0 34.0 40.0 25 37.919833485007324 40.0 38.0 40.0 33.0 40.0 26 37.76136014402441 40.0 38.0 40.0 33.0 40.0 27 37.66689784475182 40.0 37.0 40.0 33.0 40.0 28 37.50679506941789 40.0 37.0 40.0 33.0 40.0 29 37.42482299284662 40.0 37.0 40.0 33.0 40.0 30 37.29442132722787 40.0 37.0 40.0 32.0 40.0 31 37.181979027185996 40.0 36.0 40.0 32.0 40.0 32 37.00193562366921 39.0 36.0 40.0 31.0 40.0 33 37.03131147160781 40.0 36.0 40.0 32.0 40.0 34 36.91579722716289 40.0 36.0 40.0 31.0 40.0 35 36.982069758102625 40.0 36.0 40.0 31.0 40.0 36 36.97317474708482 40.0 36.0 40.0 31.0 40.0 37 36.902184357650434 40.0 36.0 40.0 31.0 40.0 38 36.80575106009305 40.0 35.0 40.0 31.0 40.0 39 36.71245551935103 40.0 35.0 40.0 31.0 40.0 40 36.623374032965096 40.0 35.0 40.0 31.0 40.0 41 36.52164139779029 40.0 35.0 40.0 30.0 40.0 42 36.40173064979226 40.0 35.0 40.0 30.0 40.0 43 36.03055773657582 39.0 35.0 40.0 29.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 2.0 10 8.0 11 18.0 12 47.0 13 44.0 14 77.0 15 97.0 16 141.0 17 361.0 18 875.0 19 2012.0 20 4520.0 21 9674.0 22 19202.0 23 34608.0 24 58419.0 25 92529.0 26 137363.0 27 198814.0 28 278017.0 29 375222.0 30 490246.0 31 610426.0 32 749040.0 33 934143.0 34 1202421.0 35 1573168.0 36 2263530.0 37 3914816.0 38 1.0858068E7 39 4347874.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.18376917452447 18.334826632241057 12.091008088817846 24.39039610441663 2 18.648375007010102 21.506139609045857 34.10655636889942 25.73892901504462 3 20.324233213475186 23.305148359752597 29.69105139075692 26.6795670360153 4 14.214802692576512 15.62758528150327 34.75650100016753 35.401111025752684 5 14.287249621152428 36.31471729981723 33.791928286988146 15.606104792042188 6 33.723160886699546 35.61767044446961 14.606121946599746 16.053046722231095 7 29.285060195271427 31.290523868577903 20.44929455522512 18.975121380925543 8 28.47855092504442 33.069934513985984 18.558876519257165 19.892638041712427 9 26.469223036702623 14.056749904628829 19.49752560601849 39.97650145265006 10 17.003526415869878 27.876237716422235 31.098151310514076 24.02208455719381 11 35.901519769491046 22.347306057870952 20.939357289406583 20.811816883231412 12 23.13818798788157 25.559850351169423 28.951032901482442 22.350928759466573 13 30.93598924242313 20.017610591756586 24.62452207420408 24.421878091616207 14 22.34675554929515 20.357981165006137 26.64919672097203 30.646066564726688 15 24.679540966770485 27.674510064237957 23.936322424419878 23.709626544571677 16 23.77092123490226 27.162189025252825 24.80608335417275 24.260806385672172 17 22.931800546978216 26.645360919282552 26.04347746251631 24.379361071222917 18 24.200626919166126 24.650467010631527 27.53653485680011 23.612371213402234 19 25.222765071033542 24.766041846536467 27.592924693303683 22.41826838912631 20 24.680247748748453 24.85822184380381 27.952662513416872 22.508867894030864 21 24.054827386615386 25.282251962234543 27.72030882607669 22.942611825073378 22 23.650558750221933 25.039491886977533 27.705416681184108 23.60453268161642 23 23.160982594588116 25.322463949945913 28.123174553518897 23.39337890194707 24 23.12266009437564 25.385903847888013 28.025642192227433 23.465793865508907 25 23.64706746035086 25.125396086480706 28.17342000398284 23.054116449185592 26 23.091899095826957 25.685842229995874 28.13669220280608 23.085566471371084 27 23.275676616771765 25.32202354308527 28.18992105458406 23.2123787855589 28 23.131823398411615 25.606693303468063 28.01971090628167 23.24177239183865 29 22.910398194218217 25.91460873242275 27.8778146571168 23.297178416242232 30 23.10759391773974 25.842033943790515 27.668546813278112 23.38182532519163 31 22.9362898556222 25.71735973387776 27.789591218258785 23.556759192241252 32 22.750470125444565 26.08576007280636 27.625884174487354 23.537885627261723 33 22.74339875399665 26.13865506777062 27.897817652593787 23.22012852563894 34 23.21190641368418 25.8755794502323 27.717481698164814 23.1950324379187 35 22.86166575442068 26.360272062048494 27.933597158352867 22.844465025177954 36 22.773602140633063 26.014740914859303 27.775910192232978 23.435746752274657 37 23.179174239267294 25.612201940894348 27.6197717534618 23.58885206637656 38 22.71041441113536 25.792257313533067 27.958458835969864 23.53886943936171 39 22.756582546470117 25.673610284608316 27.874742464096986 23.695064704824585 40 22.855073858183946 25.621301314902162 27.94120483170367 23.58241999521022 41 22.44472476577902 25.208309781333377 28.305950504022565 24.041014948865033 42 22.41097326257984 24.92235786871919 28.43122139419813 24.23544747450284 43 22.16611059973008 24.4715907918455 28.667663051672193 24.69463555675223 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4362.0 1 4696.0 2 5030.0 3 15079.5 4 25129.0 5 25129.0 6 38413.0 7 51697.0 8 50902.0 9 50107.0 10 68322.0 11 86537.0 12 86537.0 13 150852.5 14 215168.0 15 350476.0 16 485784.0 17 502134.0 18 518484.0 19 518484.0 20 493099.0 21 467714.0 22 406645.0 23 345576.0 24 402451.0 25 459326.0 26 459326.0 27 512088.5 28 564851.0 29 618956.5 30 673062.0 31 716765.5 32 760469.0 33 760469.0 34 810505.0 35 860541.0 36 922066.5 37 983592.0 38 1067675.5 39 1151759.0 40 1151759.0 41 1219017.5 42 1286276.0 43 1391231.0 44 1496186.0 45 1604228.0 46 1712270.0 47 1712270.0 48 1815636.0 49 1919002.0 50 1984750.0 51 2050498.0 52 2089790.5 53 2129083.0 54 2129083.0 55 2085616.0 56 2042149.0 57 1955600.5 58 1869052.0 59 1755319.5 60 1641587.0 61 1641587.0 62 1473022.0 63 1304457.0 64 1121365.0 65 938273.0 66 818462.0 67 698651.0 68 698651.0 69 594635.0 70 490619.0 71 417564.5 72 344510.0 73 277226.5 74 209943.0 75 209943.0 76 169181.0 77 128419.0 78 105875.5 79 83332.0 80 66914.0 81 50496.0 82 50496.0 83 39140.0 84 27784.0 85 21814.0 86 15844.0 87 11015.0 88 6186.0 89 6186.0 90 3805.0 91 1424.0 92 904.0 93 384.0 94 258.0 95 132.0 96 132.0 97 81.0 98 30.0 99 19.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 2.8155783E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.690308300508335 #Duplication Level Percentage of deduplicated Percentage of total 1 70.39190805516724 25.82700808405122 2 14.0501442963714 10.310082518009908 3 5.375128209201263 5.9164533345106065 4 2.7631295605680637 4.055203018059615 5 1.6525309101150052 3.0315934284119583 6 1.0894890020618018 2.39842124253964 7 0.7518556139372473 1.93100699909779 8 0.5822868288895204 1.7091426617025478 9 0.4070498334850164 1.3441305495812248 >10 2.569788970357378 17.677577340448238 >50 0.22112171842905867 5.620574743399985 >100 0.12984769068142224 8.88604269973175 >500 0.009198805347385697 2.310747166668003 >1k 0.005892856065067079 4.176231608066489 >5k 3.765895938620534E-4 0.9032791994189986 >10k+ 2.510597292170189E-4 3.902505406301888 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 267709 0.950813550452495 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 230098 0.8172317566163939 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 196749 0.6987871727808103 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 75776 0.2691312118721756 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.7758341154994694E-5 0.0 0.0 0.0 7.813670108197666E-5 2 2.91236794941913E-4 0.0 0.0 1.0655004692996817E-5 8.16883693129756E-5 3 4.0489017833387906E-4 0.0 0.0 1.4206672923995756E-5 8.524003754397454E-5 4 4.2264851948887376E-4 0.0 0.0 1.7758341154994694E-5 9.234337400597241E-5 5 0.0016124573768735184 0.0 0.0 2.841334584799151E-5 1.0299837869896923E-4 6 0.0023085843501493104 0.0 0.0 4.262001877198727E-5 1.5272173393295437E-4 7 0.002564304462781234 0.0 0.0 4.6171687002986206E-5 1.633767386259512E-4 8 0.0031645363938200546 0.0 0.0 6.39300281579809E-5 1.633767386259512E-4 9 0.00344866985229997 0.0 0.0 9.944671046797029E-5 1.7758341154994694E-4 10 0.005561912449744339 0.0 0.0 1.633767386259512E-4 1.9889342093594057E-4 11 0.005966802628078217 0.0 0.0 2.095484256289374E-4 2.0599675739793846E-4 12 0.00619055772663115 0.0 0.0 2.4861677616992576E-4 3.303051454829013E-4 13 0.0066487229284300136 0.0 0.0 2.699267855559194E-4 3.800285007168865E-4 14 0.0077710500894256785 0.0 0.0 3.2320180902090345E-4 4.262001877198727E-4 15 0.008552417100245445 0.0 0.0 3.551668230998939E-4 4.830268794158557E-4 16 0.009074512330202289 0.0 0.0 5.966802628078218E-4 5.22095229956844E-4 17 0.009177510708901259 0.0 0.0 9.553987541387146E-4 5.363019028808397E-4 18 0.009259199078214235 0.0 0.0 0.0012928072360836138 6.321969451178112E-4 19 0.009493609181460165 0.0 0.0 0.0018433158118884494 6.464036180418069E-4 20 0.011613955115366531 0.0 0.0 0.003327913132446006 7.280919873547825E-4 21 0.01830884973079953 0.0 0.0 0.0072560581959308324 7.565053332027741E-4 22 0.01852194982465947 0.0 0.0 0.011066998207792694 7.849186790507655E-4 23 0.018699533236209414 0.0 0.0 0.013350720880325012 8.417453707467486E-4 24 0.01911507841923629 0.0 0.0 0.01637319054490511 8.559520436707443E-4 25 0.019441831896488192 0.0 0.0 0.018021164604088615 8.666070483637411E-4 26 0.021338422731841625 0.0 0.0 0.02165807287263153 9.163304035977262E-4 27 0.021967068008728437 0.0 0.0 0.04493215479036758 9.411920812147189E-4 28 0.022457198224606293 0.0 7.103336461997878E-6 0.10351337059246407 9.518470859077156E-4 29 0.022521128252764273 0.0 1.0655004692996817E-5 0.19401342878654804 9.625020906007124E-4 30 0.02265609164554223 0.0 1.0655004692996817E-5 0.28038644849621125 9.625020906007124E-4 31 0.022989948459256133 0.0 1.0655004692996817E-5 0.33480510913157696 9.94467104679703E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 81565 0.0 19.642004 1 GTATCAA 139115 0.0 14.76383 1 TATACCG 1395 0.0 13.526882 5 TTAGGCA 9115 0.0 12.705431 4 TAGCGCA 5635 0.0 12.574091 4 TATACAG 6180 0.0 12.542881 5 TATACTG 3945 0.0 12.192651 5 AACGCAG 164885 0.0 12.169149 6 CTACTCG 8190 0.0 12.039683 14 CTTAGGC 9670 0.0 11.89969 3 TACTCGG 8300 0.0 11.790965 15 TAATACT 5865 0.0 11.418586 4 TAGTCCC 9120 0.0 11.40022 5 GCCTAAG 8200 0.0 11.303049 1 ATAGCGC 6190 0.0 11.267367 3 TATCAAC 183315 0.0 11.173773 2 TGAACCG 6845 0.0 11.162164 14 TAGGCAA 10045 0.0 11.160777 5 TAGACAG 4210 0.0 10.941806 5 TACACAG 7920 0.0 10.908461 5 >>END_MODULE