FastQCFastQC Report
Thu 26 May 2016
SRR1041754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902385
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT125720.6608546640138563No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG102050.536431899957159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT83830.4406573853347246No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC70790.3721118490736628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65150.34246485332884774No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGT60550.3182846794944241No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA58820.3091908315088691No Hit
ATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGG54640.28721841267671894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51290.2696089382538235No Hit
GGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTC50560.26577164979749107No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC50270.2642472475340165No Hit
GCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCT45040.23675544119618272No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA44910.23607208845738376No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC43700.2297116514270245No Hit
GAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGCGAAGT41430.21777926129568936No Hit
GATCTATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGG40620.21352144807701914No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC36920.19407217781889577No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT36240.19049771733902443No Hit
GGTAGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAG34250.1800371638758716No Hit
GCATTTAGGTGCAGCGTTGCGTGTTTCTTGCCGGAGGTAGAGC33020.17357159565492788No Hit
GGATAGCCGATGGGGCCCAAAAGCTTACTGACGTTAGCCAAAC31410.16510853481287963No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC30890.1623751238576839No Hit
GGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTCTCTCC30730.16153407433300832No Hit
CTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCGCG28970.15228252956157665No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG28710.1509158240839788No Hit
GTGGAGGACCGAACCCACTTCAGTTAAAAATGGAGGGGATGAG28190.14818241312878308No Hit
GTGTGACAGCGTGCCTTTTGAAGAATGAGCCTGCGAGTTAGTG26950.14166427931254713No Hit
GTGGGAAAGGATGTGGAGTTGCATAGACAACCAGGAGGTTGGC26530.1394565243102737No Hit
AGTTAGTGATATGTGGCGAGCTTAACCCGAGAGGGGAATGCGT26370.13861547478559808No Hit
CTACTGGATAGCCGATGGGGCCCAAAAGCTTACTGACGTTAGC26190.13766929407033804No Hit
AGGCTAAACCGATCATGTGTGATAGCCGGCAGGCGTTGCATGG25740.1353038422821879No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG25200.1324653001364077No Hit
AGCTGGTTCTCTCCGAAATGCATTTAGGTGCAGCGTTGCGTGT24930.13104602906351764No Hit
GGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTCTCTCCGA24110.12673565024955516No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA23810.1251586823907884No Hit
TAATAGCTCACTGGTCAAGTGATTCCGCGCCGACAATGTAACG23790.12505355120020395No Hit
CCCTTTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTG23410.12305605857909939No Hit
GGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTACCTGAAAC23390.12295092738851496No Hit
GGGATGAGCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGT22700.11932390131335141No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA22640.11900850774159806No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA22200.11669562154874014No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22100.11616996559581788No Hit
AAATAGTACCTGAAACCGTTTGCTTACAAACCGTTGGAGCACC21530.11317372666416103No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG21520.11312116106886883No Hit
GTCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGA21490.11296346428299214No Hit
ATAGCTCACTGGTCAAGTGATTCCGCGCCGACAATGTAACGGG21220.11154419321010205No Hit
GGCATGTAGAGCCGAAGAAGGACGTAGTAATCTGCGATAAGCC21110.11096597166188758No Hit
GAGGTAGAGCTACTGGATAGCCGATGGGGCCCAAAAGCTTACT20950.11012492213721198No Hit
GAATATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACAT20910.10991465975604307No Hit
GGTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGG20900.10986209416075085No Hit
GTAGTAATCTGCGATAAGCCTCGGGGAGTTGATAAACGAACTG20400.10723381439613959No Hit
ATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACATCTCA20280.10660302725263288No Hit
GGTTGAAGCGACGGTAAGACGTCGTGGAGGACCGAACCCACTT20250.1064453304667562No Hit
GGTCAAGTGATTCCGCGCCGACAATGTAACGGGGCTCAAGCAC20220.10628763368087951No Hit
AAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC19950.10486836260798944No Hit
GTGATATGTGGCGAGCTTAACCCGAGAGGGGAATGCGTAGCGA19880.10450040344094386No Hit
TTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTGCGAG19830.10423757546448274No Hit
GTGCAGCGTTGCGTGTTTCTTGCCGGAGGTAGAGCTACTGGAT19440.10218751724808595No Hit
ATGTAACGGGGCTCAAGCACGCCACCGAAGTTGTGGCATTGAT19360.10176699248574815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAT7000.018.519
TAACCTA1054.80135E-717.619055
CTACGCA7350.017.61904718
CGCTACG7700.016.81818216
ATCCCCT5100.016.3235312
GTACGAC803.3840231E-416.18753
GCTACGC8050.016.08695617
TCGAAAT13750.016.0109126
AGTCGAA14200.015.89436624
CGCATTC8250.015.6969721
ACGCATT8500.015.670588520
GTCGAAA15000.014.79999925
GGCGTAC1001.0939622E-414.7999991
TACGCAC3300.014.5757573
ATTACGC3050.014.5573771
TTCGCTA9050.014.51381214
CGTACAC908.277529E-414.3888883
CAATGGC15500.014.32257937
GGTCTAT1304.447358E-614.2307691
ATTCCCC9150.013.95081924
Produced by FastQC (version 0.11.5)