##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041754_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1902385
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.54236287607398	31.0	30.0	31.0	27.0	34.0
2	30.726294624905055	31.0	30.0	33.0	27.0	34.0
3	30.62279822433419	31.0	30.0	33.0	27.0	34.0
4	34.24606480812243	35.0	35.0	37.0	30.0	37.0
5	33.70226163473745	35.0	33.0	37.0	28.0	37.0
6	34.32559970773529	35.0	35.0	37.0	30.0	37.0
7	34.39046407535804	35.0	35.0	37.0	30.0	37.0
8	34.42207176780725	35.0	35.0	37.0	30.0	37.0
9	35.90387066760934	37.0	35.0	39.0	30.0	39.0
10	35.51926397653472	37.0	35.0	39.0	30.0	39.0
11	35.84077723489199	37.0	35.0	39.0	30.0	39.0
12	35.506823277096906	37.0	35.0	39.0	30.0	39.0
13	35.61434988185882	37.0	35.0	39.0	30.0	39.0
14	36.16795548745391	38.0	35.0	40.0	30.0	40.0
15	36.229898784946265	38.0	35.0	40.0	30.0	40.0
16	36.123124393852976	38.0	35.0	40.0	30.0	40.0
17	36.125814175364084	38.0	35.0	40.0	30.0	40.0
18	35.99434604457037	38.0	34.0	40.0	30.0	40.0
19	36.09264423342278	38.0	34.0	40.0	30.0	40.0
20	36.016287449701295	38.0	34.0	40.0	29.0	40.0
21	35.975380903444886	38.0	34.0	40.0	29.0	40.0
22	35.85525695377119	38.0	34.0	40.0	29.0	40.0
23	35.739993219038205	38.0	34.0	40.0	29.0	40.0
24	35.62231935176108	38.0	34.0	40.0	27.0	40.0
25	35.554143877290876	38.0	34.0	40.0	27.0	40.0
26	35.21284177492989	38.0	34.0	40.0	27.0	40.0
27	35.06420361808993	38.0	33.0	40.0	27.0	40.0
28	34.97432696325928	38.0	33.0	40.0	26.0	40.0
29	34.71310486573433	37.0	33.0	40.0	25.0	40.0
30	34.59522021042008	37.0	33.0	40.0	25.0	40.0
31	34.4217385019331	37.0	33.0	39.0	25.0	40.0
32	34.271193790951884	37.0	32.0	39.0	25.0	40.0
33	34.21616812579998	37.0	32.0	39.0	25.0	40.0
34	34.02615033234598	37.0	32.0	39.0	24.0	40.0
35	33.89905671039248	37.0	31.0	39.0	24.0	40.0
36	33.91969554007207	37.0	31.0	39.0	24.0	40.0
37	33.911832252672305	37.0	31.0	39.0	24.0	40.0
38	33.86397548340636	37.0	31.0	39.0	24.0	40.0
39	33.73855449869506	37.0	31.0	39.0	23.0	40.0
40	33.60507993912904	37.0	31.0	39.0	23.0	40.0
41	33.49973638353961	36.0	31.0	39.0	23.0	40.0
42	33.26857549865038	36.0	31.0	39.0	22.0	40.0
43	33.032958628248224	36.0	30.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	9.0
14	12.0
15	48.0
16	126.0
17	232.0
18	467.0
19	949.0
20	1771.0
21	3191.0
22	5634.0
23	9212.0
24	14064.0
25	20756.0
26	28853.0
27	39503.0
28	51198.0
29	65283.0
30	81089.0
31	98517.0
32	118237.0
33	141615.0
34	170284.0
35	201078.0
36	235797.0
37	266790.0
38	311884.0
39	35781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.06678196053901	25.056862832707367	14.47030963763907	17.40604556911456
2	24.048970108574235	25.877201512837832	31.7656520630682	18.308176315519727
3	26.878891496726474	25.78983749346215	27.578224176494242	19.753046833317125
4	23.73814974361131	20.315498702943934	30.477900109599265	25.468451443845492
5	16.284611159150224	40.42846216722693	31.056647313766668	12.23027935985618
6	32.981284019796206	38.724548395829444	14.532810130441526	13.761357453932826
7	28.531028156761117	32.89602262423222	20.140928360978457	18.43202085802821
8	28.238395487769303	36.207970521214165	17.08292485485325	18.47070913616329
9	29.089642737931598	15.507744226326428	18.059225656215748	37.34338737952623
10	19.069378700946444	29.092744108053836	27.673788428735506	24.16408876226421
11	38.14112285368104	24.072361798479278	19.284897641644566	18.501617706195116
12	25.434809462858464	25.906375418225018	26.81160753475243	21.84720758416409
13	32.647282227309404	21.395774251794457	23.71028997810643	22.24665354278971
14	24.985478754300523	21.508001797743358	26.136402463223796	27.370116984732324
15	28.424793088675532	27.292687862866877	21.928631691271747	22.353887357185847
16	26.666421360555304	27.595781085321846	22.38490105840826	23.35289649571459
17	27.13577956091958	27.677730848382424	23.558270276521316	21.62821931417668
18	26.416051430178435	25.637397267114704	25.57258388811939	22.37396741458748
19	28.593633780754157	26.185078204464396	24.062111507397294	21.159176507384153
20	26.206524967343626	26.88062616137112	24.542823876344695	22.370024994940565
21	27.556514585638553	26.07884313637881	23.721696712284842	22.642945565697794
22	25.19001148558257	27.051990002023775	24.264541614867653	23.493456897526
23	26.258091816325297	25.516706660323752	25.592453683139848	22.632747840211103
24	24.61336690522686	26.65217608423111	25.02574400029437	23.708713010247664
25	26.016290077981058	27.215574134573178	25.533475085221973	21.23466070222379
26	26.10764908259895	26.310447149236353	25.56222846584682	22.01967530231788
27	26.834105609537502	27.46573380256888	24.573206790423598	21.12695379747002
28	27.842629120814138	26.20831219758356	23.786930616042497	22.16212806555981
29	26.48154816191255	26.123155933210157	25.593820388617445	21.801475516259853
30	25.34292480228765	28.28018513602662	24.12808132948904	22.24880873219669
31	25.04335347471726	25.93544419242162	25.510871879246316	23.510330453614806
32	26.313548519358594	26.720563923706298	24.272426454161486	22.693461102773625
33	27.914643986364485	25.9734491178179	24.42160761360082	21.690299282216795
34	27.38772645915522	26.22907560772399	23.50433797575149	22.8788599573693
35	27.57932805399538	28.10703406513403	23.095693037949733	21.21794484292086
36	26.6359858808811	26.6123313629996	25.028792804821315	21.722889951297976
37	26.66989068984459	26.249786452269124	23.743143475164068	23.337179382722216
38	26.484596966439494	26.361540907860398	24.32898703469592	22.824875091004188
39	26.108647828909497	25.913419207994178	24.84323625343976	23.134696709656563
40	26.300039161368495	26.638403898264546	25.164044081508212	21.897512858858747
41	28.63274258365157	25.254824864577884	24.130657043658353	21.981775508112186
42	27.7576831188219	25.80056087490177	24.499141866656853	21.94261413961948
43	27.02460332687653	24.64028049001648	25.06290787616597	23.272208306941025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	192.0
2	219.0
3	373.5
4	528.0
5	528.0
6	865.0
7	1202.0
8	1098.0
9	994.0
10	1502.5
11	2011.0
12	2011.0
13	3700.5
14	5390.0
15	10110.5
16	14831.0
17	13104.5
18	11378.0
19	11378.0
20	12854.5
21	14331.0
22	13127.5
23	11924.0
24	13795.5
25	15667.0
26	15667.0
27	17765.0
28	19863.0
29	23376.0
30	26889.0
31	31149.0
32	35409.0
33	35409.0
34	40125.0
35	44841.0
36	50045.5
37	55250.0
38	64188.0
39	73126.0
40	73126.0
41	80955.0
42	88784.0
43	100207.5
44	111631.0
45	127778.0
46	143925.0
47	143925.0
48	180020.0
49	216115.0
50	221564.5
51	227014.0
52	234682.5
53	242351.0
54	242351.0
55	212928.0
56	183505.0
57	152501.5
58	121498.0
59	100268.5
60	79039.0
61	79039.0
62	65362.5
63	51686.0
64	45235.5
65	38785.0
66	31477.5
67	24170.0
68	24170.0
69	19718.5
70	15267.0
71	12936.5
72	10606.0
73	8224.5
74	5843.0
75	5843.0
76	4658.0
77	3473.0
78	2786.5
79	2100.0
80	1660.0
81	1220.0
82	1220.0
83	928.0
84	636.0
85	533.5
86	431.0
87	317.0
88	203.0
89	203.0
90	133.0
91	63.0
92	40.0
93	17.0
94	11.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1902385.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.49171976087658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.57703325933736	38.02062975479236
2	9.354504120134557	8.511049598702533
3	2.8618722004572517	3.9057446440393355
4	1.269864509737047	2.310732816449627
5	0.7196859771635729	1.6369876394478937
6	0.43336231304652567	1.182863814002269
7	0.31433032182621357	1.0009598839004978
8	0.21701532864091944	0.7897920411477792
9	0.1603651939560111	0.6565759616560929
>10	0.8887580364610532	7.387666553457615
>50	0.07522393066156233	2.3985113908156586
>100	0.09303802919098504	9.496244991208977
>500	0.018399132193865644	5.725767557025472
>1k	0.015274751255284685	12.903717960366997
>5k	0.0010414603128603194	2.8737280352495267
>10k+	2.31435625080071E-4	1.1990273577372863
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	12572	0.6608546640138563	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	10205	0.536431899957159	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8383	0.4406573853347246	No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC	7079	0.3721118490736628	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6515	0.34246485332884774	No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGT	6055	0.3182846794944241	No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA	5882	0.3091908315088691	No Hit
ATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGG	5464	0.28721841267671894	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5129	0.2696089382538235	No Hit
GGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTC	5056	0.26577164979749107	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	5027	0.2642472475340165	No Hit
GCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCT	4504	0.23675544119618272	No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA	4491	0.23607208845738376	No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC	4370	0.2297116514270245	No Hit
GAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGCGAAGT	4143	0.21777926129568936	No Hit
GATCTATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGG	4062	0.21352144807701914	No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC	3692	0.19407217781889577	No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT	3624	0.19049771733902443	No Hit
GGTAGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAG	3425	0.1800371638758716	No Hit
GCATTTAGGTGCAGCGTTGCGTGTTTCTTGCCGGAGGTAGAGC	3302	0.17357159565492788	No Hit
GGATAGCCGATGGGGCCCAAAAGCTTACTGACGTTAGCCAAAC	3141	0.16510853481287963	No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC	3089	0.1623751238576839	No Hit
GGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTCTCTCC	3073	0.16153407433300832	No Hit
CTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCGCG	2897	0.15228252956157665	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	2871	0.1509158240839788	No Hit
GTGGAGGACCGAACCCACTTCAGTTAAAAATGGAGGGGATGAG	2819	0.14818241312878308	No Hit
GTGTGACAGCGTGCCTTTTGAAGAATGAGCCTGCGAGTTAGTG	2695	0.14166427931254713	No Hit
GTGGGAAAGGATGTGGAGTTGCATAGACAACCAGGAGGTTGGC	2653	0.1394565243102737	No Hit
AGTTAGTGATATGTGGCGAGCTTAACCCGAGAGGGGAATGCGT	2637	0.13861547478559808	No Hit
CTACTGGATAGCCGATGGGGCCCAAAAGCTTACTGACGTTAGC	2619	0.13766929407033804	No Hit
AGGCTAAACCGATCATGTGTGATAGCCGGCAGGCGTTGCATGG	2574	0.1353038422821879	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	2520	0.1324653001364077	No Hit
AGCTGGTTCTCTCCGAAATGCATTTAGGTGCAGCGTTGCGTGT	2493	0.13104602906351764	No Hit
GGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTCTCTCCGA	2411	0.12673565024955516	No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA	2381	0.1251586823907884	No Hit
TAATAGCTCACTGGTCAAGTGATTCCGCGCCGACAATGTAACG	2379	0.12505355120020395	No Hit
CCCTTTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTG	2341	0.12305605857909939	No Hit
GGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTACCTGAAAC	2339	0.12295092738851496	No Hit
GGGATGAGCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGT	2270	0.11932390131335141	No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA	2264	0.11900850774159806	No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA	2220	0.11669562154874014	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2210	0.11616996559581788	No Hit
AAATAGTACCTGAAACCGTTTGCTTACAAACCGTTGGAGCACC	2153	0.11317372666416103	No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG	2152	0.11312116106886883	No Hit
GTCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGA	2149	0.11296346428299214	No Hit
ATAGCTCACTGGTCAAGTGATTCCGCGCCGACAATGTAACGGG	2122	0.11154419321010205	No Hit
GGCATGTAGAGCCGAAGAAGGACGTAGTAATCTGCGATAAGCC	2111	0.11096597166188758	No Hit
GAGGTAGAGCTACTGGATAGCCGATGGGGCCCAAAAGCTTACT	2095	0.11012492213721198	No Hit
GAATATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACAT	2091	0.10991465975604307	No Hit
GGTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGG	2090	0.10986209416075085	No Hit
GTAGTAATCTGCGATAAGCCTCGGGGAGTTGATAAACGAACTG	2040	0.10723381439613959	No Hit
ATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACATCTCA	2028	0.10660302725263288	No Hit
GGTTGAAGCGACGGTAAGACGTCGTGGAGGACCGAACCCACTT	2025	0.1064453304667562	No Hit
GGTCAAGTGATTCCGCGCCGACAATGTAACGGGGCTCAAGCAC	2022	0.10628763368087951	No Hit
AAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC	1995	0.10486836260798944	No Hit
GTGATATGTGGCGAGCTTAACCCGAGAGGGGAATGCGTAGCGA	1988	0.10450040344094386	No Hit
TTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTGCGAG	1983	0.10423757546448274	No Hit
GTGCAGCGTTGCGTGTTTCTTGCCGGAGGTAGAGCTACTGGAT	1944	0.10218751724808595	No Hit
ATGTAACGGGGCTCAAGCACGCCACCGAAGTTGTGGCATTGAT	1936	0.10176699248574815	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.2565595292225286E-5	0.0
3	0.0	0.0	0.0	1.5769678587667586E-4	0.0
4	0.0	0.0	0.0	1.5769678587667586E-4	0.0
5	2.1026238116890114E-4	0.0	0.0	6.833527387989287E-4	0.0
6	3.67959167045577E-4	0.0	0.0	9.987463105522805E-4	0.0
7	4.7309035763002755E-4	0.0	0.0	9.987463105522805E-4	0.0
8	7.884839293833793E-4	0.0	0.0	0.0014192710728900828	0.0
9	7.884839293833793E-4	0.0	0.0	0.001471836668182308	0.0
10	0.001103877501136731	0.0	0.0	0.002418017383442363	0.0
11	0.0012090086917211816	0.0	0.0	0.002418017383442363	0.0
12	0.0012615742870134069	0.0	0.0	0.002575714169319039	0.0
13	0.0012615742870134069	0.0	0.0	0.0028385421457801656	0.0
14	0.0013141398823056322	0.0	0.0	0.0032065013128257424	0.0
15	0.0014192710728900828	0.0	0.0	0.0034167636939946436	0.0
16	0.0015244022634745332	0.0	0.0	0.003469329289286869	0.0
17	0.0015244022634745332	0.0	0.0	0.0035218948845790942	0.0
18	0.0015769678587667585	0.0	0.0	0.0035218948845790942	0.0
19	0.0016295334540589838	0.0	0.0	0.00367959167045577	0.0
20	0.002207755002273462	0.0	0.0	0.004415510004546924	0.0
21	0.0028385421457801656	0.0	0.0	0.0057296498868525565	0.0
22	0.0028385421457801656	0.0	0.0	0.005887346672729232	0.0
23	0.002891107741072391	0.0	0.0	0.005939912268021457	5.2565595292225286E-5
24	0.002996238931656841	0.0	0.0	0.006097609053898133	5.2565595292225286E-5
25	0.0030488045269490664	0.0	0.0	0.006150174649190358	5.2565595292225286E-5
26	0.0032065013128257424	0.0	0.0	0.006938658578573738	5.2565595292225286E-5
27	0.0033641980987024183	0.0	0.0	0.007306617745619315	5.2565595292225286E-5
28	0.00367959167045577	0.0	0.0	0.007832273698541567	5.2565595292225286E-5
29	0.00367959167045577	0.0	0.0	0.008725888818509397	5.2565595292225286E-5
30	0.00367959167045577	0.0	0.0	0.010565684653737282	5.2565595292225286E-5
31	0.0037321572657479955	0.0	0.0	0.01293113644188742	5.2565595292225286E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAT	700	0.0	18.5	19
TAACCTA	105	4.80135E-7	17.61905	5
CTACGCA	735	0.0	17.619047	18
CGCTACG	770	0.0	16.818182	16
ATCCCCT	510	0.0	16.32353	12
GTACGAC	80	3.3840231E-4	16.1875	3
GCTACGC	805	0.0	16.086956	17
TCGAAAT	1375	0.0	16.01091	26
AGTCGAA	1420	0.0	15.894366	24
CGCATTC	825	0.0	15.69697	21
ACGCATT	850	0.0	15.6705885	20
GTCGAAA	1500	0.0	14.799999	25
GGCGTAC	100	1.0939622E-4	14.799999	1
TACGCAC	330	0.0	14.575757	3
ATTACGC	305	0.0	14.557377	1
TTCGCTA	905	0.0	14.513812	14
CGTACAC	90	8.277529E-4	14.388888	3
CAATGGC	1550	0.0	14.322579	37
GGTCTAT	130	4.447358E-6	14.230769	1
ATTCCCC	915	0.0	13.950819	24
>>END_MODULE