FastQCFastQC Report
Thu 26 May 2016
SRR1041753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4098671
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT157990.38546641094149786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129240.31532172257787955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96960.23656448639083255No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT96090.23444184712556826No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC86500.21104401890271263No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC70000.17078706732011426No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA59600.14541298874684014No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG58090.14172886772322052No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC53080.1295053933335952No Hit
ATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGG48560.11847742841521068No Hit
GAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGCGAAGT46650.11381738129261901No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGT46540.11354900161540168No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC42630.10400932399794958No Hit
GGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTC41800.1019842773425825No Hit
GATCTATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGG41320.10081316602381601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAAT21500.017.63953426
TACGCAT10450.017.17224919
CGCATTC10650.017.02347421
CGTATAC1201.0430995E-716.9583323
GTCGAAA22900.016.56113425
AGTCGAA23950.016.06680724
TAGTCGA24650.015.68559823
ACGCATT11850.015.611814520
CCCGTAG24250.015.48659719
CCGTAGT24250.015.48659720
CGCTACG11750.015.42978816
CCCCGTA24300.015.378618
TGCGAAC23600.015.36440712
GTGCGAA23750.015.34526311
CTACGCA12000.015.10833418
GCTACGC12150.014.9218117
GTAGTCG25350.014.81459622
CGGGTTA11700.014.70512934
CGTAGTC26000.014.65769221
ACCCCGT25550.014.62622417
Produced by FastQC (version 0.11.5)