##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041753_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4098671
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.876128872017297	31.0	31.0	31.0	28.0	34.0
2	31.122393819850387	31.0	31.0	34.0	28.0	34.0
3	31.131785156700793	31.0	31.0	34.0	28.0	34.0
4	34.873257453452595	35.0	35.0	37.0	32.0	37.0
5	34.53912085161263	35.0	35.0	37.0	30.0	37.0
6	34.90552205824766	35.0	35.0	37.0	32.0	37.0
7	34.90047066475938	35.0	35.0	37.0	32.0	37.0
8	34.90203824605585	35.0	35.0	37.0	32.0	37.0
9	36.41373240252755	38.0	35.0	39.0	32.0	39.0
10	36.12310819775483	37.0	35.0	39.0	32.0	39.0
11	36.343292740500516	38.0	35.0	39.0	32.0	39.0
12	36.08356733194736	37.0	35.0	39.0	31.0	39.0
13	36.156256747614044	37.0	35.0	39.0	32.0	39.0
14	36.844773098401895	38.0	36.0	40.0	31.0	40.0
15	36.86809431642598	38.0	36.0	40.0	32.0	40.0
16	36.80588463919158	38.0	36.0	40.0	31.0	40.0
17	36.77373885339907	38.0	36.0	40.0	31.0	40.0
18	36.69811751174954	38.0	36.0	40.0	31.0	40.0
19	36.76339281684234	39.0	36.0	40.0	31.0	40.0
20	36.68172561300968	38.0	36.0	40.0	31.0	40.0
21	36.62859814803384	38.0	36.0	40.0	31.0	40.0
22	36.53610670385596	38.0	36.0	40.0	31.0	40.0
23	36.445999935100915	38.0	35.0	40.0	30.0	40.0
24	36.35957509153577	38.0	35.0	40.0	30.0	40.0
25	36.28278995801322	38.0	35.0	40.0	30.0	40.0
26	36.02316628975588	38.0	35.0	40.0	30.0	40.0
27	35.89284160646219	38.0	34.0	40.0	29.0	40.0
28	35.800350406265835	38.0	34.0	40.0	29.0	40.0
29	35.6391998772285	38.0	34.0	40.0	28.0	40.0
30	35.51386510407886	38.0	34.0	40.0	27.0	40.0
31	35.35785014215583	38.0	34.0	40.0	27.0	40.0
32	35.19576150415586	38.0	34.0	40.0	27.0	40.0
33	35.11402159382883	38.0	33.0	40.0	27.0	40.0
34	34.958020050889665	38.0	33.0	40.0	26.0	40.0
35	34.842109064133226	38.0	33.0	40.0	26.0	40.0
36	34.90927815382108	38.0	33.0	40.0	26.0	40.0
37	34.90630206718227	38.0	33.0	40.0	26.0	40.0
38	34.84658100150024	38.0	33.0	40.0	26.0	40.0
39	34.745092738597464	38.0	33.0	40.0	26.0	40.0
40	34.65786446387134	37.0	33.0	40.0	25.0	40.0
41	34.545222829546454	37.0	33.0	40.0	25.0	40.0
42	34.40544069040916	37.0	33.0	40.0	25.0	40.0
43	34.2148796524532	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	5.0
15	23.0
16	42.0
17	117.0
18	344.0
19	713.0
20	1523.0
21	3171.0
22	6100.0
23	10736.0
24	17686.0
25	27521.0
26	40411.0
27	57183.0
28	76855.0
29	101842.0
30	130467.0
31	162846.0
32	202907.0
33	255475.0
34	326499.0
35	430651.0
36	586581.0
37	774769.0
38	818978.0
39	65222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.595810446849725	25.25369808896591	15.352757027826824	19.79773443635754
2	21.07519730175952	25.917888993773836	33.40853657197662	19.59837713249002
3	23.32607325642873	26.752135021327643	28.821488721588047	21.10030300065558
4	18.783161663866167	21.364242214122577	33.13488689382485	26.717709228186404
5	14.750073865406616	39.15132490507289	33.06798715974031	13.03061406978018
6	31.398153206246608	39.43190365852736	15.57402387261627	13.595919262609756
7	27.37182369602244	32.297688689821655	21.678856390278703	18.651631223877203
8	26.427566398962004	36.14317909390629	18.631673535153222	18.797580971978476
9	27.26530136231964	15.082010729819494	19.025728095765675	38.626959812095194
10	16.813767194292982	28.617105398310816	30.96803817627714	23.60108923111906
11	36.40033562098544	23.588158210307682	21.43026361471804	18.58124255398884
12	23.51335347482147	25.477673128680006	29.173846839621913	21.83512655687661
13	30.191420584867636	21.534565716545682	25.243792439061348	23.030221259525344
14	22.94146078082383	20.743675205938704	27.009096363186995	29.30576765005047
15	25.637554221844105	27.936201759058	22.864630998682255	23.561613020415642
16	24.71786586432529	27.759119968399514	23.718907909417467	23.80410625785773
17	24.395785853512027	27.235950384893055	25.57026411731998	22.797999644274938
18	24.737969941964113	26.224305390698593	26.29608475527799	22.7416399120593
19	26.3438319396702	26.15013500717672	25.753957807299	21.752075245854083
20	24.89345936768284	26.66261819989943	25.677250015919796	22.766672416497933
21	25.412847237555784	26.1306896796547	25.42977955537295	23.02668352741657
22	24.159050580053876	26.59149758543684	25.92413492080726	23.325316913702025
23	24.230610361261004	25.750468871495176	26.714244690535054	23.304676076708766
24	23.667013039104624	26.390408012743645	26.48958650255168	23.45299244560005
25	24.377487239156302	26.661837459020255	26.669254497372442	22.291420804451004
26	24.259766153467798	26.448963578681965	26.561585450503344	22.72968481734689
27	24.736847626950297	26.834503184080887	25.74527206501815	22.683377123950667
28	25.136269781107096	26.269295583861208	25.772085634587405	22.82234900044429
29	24.59814413013389	26.109121712867417	26.149378664450012	23.143355492548682
30	23.90643210933495	27.446384449983906	25.697232102796246	22.94995133788489
31	24.070412092114736	26.359178377576537	26.29076595803859	23.279643572270135
32	24.692540582056964	26.436715706139868	25.82190666193993	23.048837049863234
33	25.338506066966588	26.242384421682054	25.824590458712105	22.59451905263926
34	25.139831911368343	26.200078025291614	25.32872240782439	23.331367655515653
35	24.98085354984579	27.286381366057437	25.108309498371543	22.624455585725226
36	24.7122542892562	26.371621435338433	26.084674764088163	22.831449511317206
37	25.111139684058564	26.023313410615295	25.360269218973663	23.50527768635248
38	24.626421588851606	26.2843004476329	25.81856411505095	23.27071384846454
39	24.535343285665036	25.954388629875393	26.054055082732912	23.45621300172666
40	24.62361580131706	26.36520472123769	26.23330830896161	22.77787116848364
41	25.54359693666557	25.760276928789843	25.85616166801385	22.839964466530738
42	25.515075496423105	25.873264772898334	25.70384400211678	22.90781572856177
43	24.90670756447639	25.31335157176558	26.223451455361996	23.55648940839604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	285.0
1	377.5
2	470.0
3	839.0
4	1208.0
5	1208.0
6	1931.0
7	2654.0
8	2373.5
9	2093.0
10	3119.0
11	4145.0
12	4145.0
13	7067.5
14	9990.0
15	19483.5
16	28977.0
17	28652.0
18	28327.0
19	28327.0
20	30641.0
21	32955.0
22	34763.0
23	36571.0
24	44198.5
25	51826.0
26	51826.0
27	59881.0
28	67936.0
29	78372.0
30	88808.0
31	101292.5
32	113777.0
33	113777.0
34	127142.5
35	140508.0
36	154692.0
37	168876.0
38	187858.0
39	206840.0
40	206840.0
41	222595.0
42	238350.0
43	258015.5
44	277681.0
45	303008.5
46	328336.0
47	328336.0
48	359158.5
49	389981.0
50	394244.5
51	398508.0
52	396904.0
53	395300.0
54	395300.0
55	364788.0
56	334276.0
57	284019.5
58	233763.0
59	197570.5
60	161378.0
61	161378.0
62	137079.5
63	112781.0
64	100237.5
65	87694.0
66	71165.5
67	54637.0
68	54637.0
69	45209.5
70	35782.0
71	31636.5
72	27491.0
73	21298.5
74	15106.0
75	15106.0
76	12393.0
77	9680.0
78	7481.5
79	5283.0
80	4152.5
81	3022.0
82	3022.0
83	2346.0
84	1670.0
85	1393.0
86	1116.0
87	755.0
88	394.0
89	394.0
90	270.5
91	147.0
92	90.0
93	33.0
94	23.5
95	14.0
96	14.0
97	8.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4098671.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.03177139286327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57609900463909	42.445489345309625
2	10.563129330169248	10.992366610012304
3	3.260112333095322	5.088882588920097
4	1.4017162458862313	2.917351170544596
5	0.8135945705474955	2.1166383350601023
6	0.4815266167979596	1.5032809706886183
7	0.3361685308188398	1.2244010901528406
8	0.23556527204657216	0.9805502706579908
9	0.18551474649982566	0.8687394791895517
>10	0.9994514842821749	9.316006875522634
>50	0.07189333018043328	2.577731800864376
>100	0.05629027655756915	6.335505242844003
>500	0.01026211054575187	3.731671384012889
>1k	0.008256334323972745	7.941403042965748
>5k	3.265216964283005E-4	1.2628539739536055
>10k+	9.329191326522873E-5	0.6971278193009958
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15799	0.38546641094149786	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12924	0.31532172257787955	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9696	0.23656448639083255	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	9609	0.23444184712556826	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	8650	0.21104401890271263	No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC	7000	0.17078706732011426	No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA	5960	0.14541298874684014	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	5809	0.14172886772322052	No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC	5308	0.1295053933335952	No Hit
ATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGG	4856	0.11847742841521068	No Hit
GAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGCGAAGT	4665	0.11381738129261901	No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGT	4654	0.11354900161540168	No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC	4263	0.10400932399794958	No Hit
GGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTC	4180	0.1019842773425825	No Hit
GATCTATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGG	4132	0.10081316602381601	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.4398152474302036E-5	0.0	0.0	0.0
5	0.0	2.4398152474302036E-5	0.0	2.4398152474302036E-5	2.4398152474302036E-5
6	4.879630494860407E-5	2.4398152474302036E-5	0.0	7.31944574229061E-5	2.4398152474302036E-5
7	4.879630494860407E-5	2.4398152474302036E-5	0.0	1.2199076237151018E-4	2.4398152474302036E-5
8	7.31944574229061E-5	2.4398152474302036E-5	0.0	1.2199076237151018E-4	2.4398152474302036E-5
9	7.31944574229061E-5	2.4398152474302036E-5	0.0	1.463889148458122E-4	2.4398152474302036E-5
10	1.2199076237151018E-4	2.4398152474302036E-5	0.0	1.463889148458122E-4	2.4398152474302036E-5
11	1.463889148458122E-4	2.4398152474302036E-5	0.0	1.463889148458122E-4	2.4398152474302036E-5
12	1.463889148458122E-4	2.4398152474302036E-5	0.0	1.951852197944163E-4	2.4398152474302036E-5
13	1.463889148458122E-4	2.4398152474302036E-5	0.0	2.1958337226871832E-4	2.4398152474302036E-5
14	1.463889148458122E-4	2.4398152474302036E-5	0.0	2.683796772173224E-4	2.4398152474302036E-5
15	2.1958337226871832E-4	2.4398152474302036E-5	0.0	2.683796772173224E-4	2.4398152474302036E-5
16	2.683796772173224E-4	2.4398152474302036E-5	0.0	2.927778296916244E-4	2.4398152474302036E-5
17	2.683796772173224E-4	2.4398152474302036E-5	0.0	3.1717598216592644E-4	2.4398152474302036E-5
18	2.683796772173224E-4	2.4398152474302036E-5	0.0	3.1717598216592644E-4	2.4398152474302036E-5
19	2.683796772173224E-4	2.4398152474302036E-5	0.0	4.147685920631346E-4	4.879630494860407E-5
20	2.927778296916244E-4	2.4398152474302036E-5	0.0	5.611575069089468E-4	4.879630494860407E-5
21	4.635648970117387E-4	2.4398152474302036E-5	0.0	6.343519643318529E-4	4.879630494860407E-5
22	4.635648970117387E-4	2.4398152474302036E-5	0.0	6.58750116806155E-4	4.879630494860407E-5
23	4.635648970117387E-4	2.4398152474302036E-5	0.0	7.07546421754759E-4	4.879630494860407E-5
24	4.635648970117387E-4	2.4398152474302036E-5	0.0	8.051390316519671E-4	4.879630494860407E-5
25	4.635648970117387E-4	2.4398152474302036E-5	0.0	8.295371841262692E-4	4.879630494860407E-5
26	4.635648970117387E-4	2.4398152474302036E-5	0.0	9.271297940234773E-4	4.879630494860407E-5
27	4.635648970117387E-4	2.4398152474302036E-5	0.0	0.0010979168613435917	4.879630494860407E-5
28	5.367593544346448E-4	2.4398152474302036E-5	0.0	0.0018054632830983507	4.879630494860407E-5
29	5.367593544346448E-4	2.4398152474302036E-5	0.0	0.003391343193927983	4.879630494860407E-5
30	5.367593544346448E-4	2.4398152474302036E-5	0.0	0.005977547356203999	4.879630494860407E-5
31	5.611575069089468E-4	2.4398152474302036E-5	0.0	0.010393612954052667	4.879630494860407E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAAAT	2150	0.0	17.639534	26
TACGCAT	1045	0.0	17.172249	19
CGCATTC	1065	0.0	17.023474	21
CGTATAC	120	1.0430995E-7	16.958332	3
GTCGAAA	2290	0.0	16.561134	25
AGTCGAA	2395	0.0	16.066807	24
TAGTCGA	2465	0.0	15.685598	23
ACGCATT	1185	0.0	15.6118145	20
CCCGTAG	2425	0.0	15.486597	19
CCGTAGT	2425	0.0	15.486597	20
CGCTACG	1175	0.0	15.429788	16
CCCCGTA	2430	0.0	15.3786	18
TGCGAAC	2360	0.0	15.364407	12
GTGCGAA	2375	0.0	15.345263	11
CTACGCA	1200	0.0	15.108334	18
GCTACGC	1215	0.0	14.92181	17
GTAGTCG	2535	0.0	14.814596	22
CGGGTTA	1170	0.0	14.705129	34
CGTAGTC	2600	0.0	14.657692	21
ACCCCGT	2555	0.0	14.626224	17
>>END_MODULE