FastQCFastQC Report
Thu 26 May 2016
SRR1041752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7032176
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC550480.7828017956319637No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT325900.4634411880476257No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC320610.4559186231971441No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA249540.3548545997711093No Hit
CTCTATGACCAGGCTTTCAATCCTGTTCGTCTATACAACGTTG247900.3525224624639656No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT233860.33255709185890686No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC232950.3312630400604308No Hit
GTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTGCGA232600.3307653278302477No Hit
ATCCACAGCTCATCCCCTCCATTTTTAACTGAAGTGGGTTCGG215690.30671871693768754No Hit
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTA215230.30606458086373267No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA204280.29049329823371883No Hit
GAATATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACAT204210.29039375578768223No Hit
ATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGG198060.28164824088589363No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG189230.26909167233584597No Hit
AGGCTAAACCGATCATGTGTGATAGCCGGCAGGCGTTGCATGG185600.26392968549137563No Hit
GTATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGA178720.2541460850809195No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCC174250.24778958888400973No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCG173190.24628223184402667No Hit
AACAAAGGGTGCTCCAACGGTTTGTAAGCAAACGGTTTCAGGT172620.2454716719262999No Hit
GAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGCGAAGT170200.2420303473633197No Hit
ATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACATCTCA170010.24176016072407744No Hit
GGATAGATCACTTCGCTTCGGGTCTAGGACATGCGACTGAATC169960.24168905897690843No Hit
TCTCAGTACCCACAGGAAGAGAAAACAATAGTGATTCCGTTAG167740.2385321414026043No Hit
GCCTAAATACTCCTACATGACCGATAGTGAACAAGTACCGTGA166720.2370816657603564No Hit
TATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGAC165240.23497705404415364No Hit
GGTTTACACCGCCGCTTCGCTGGCCACACGACGCTCTCCTACC157690.2242406902216327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT157590.22409848672729468No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGT156730.2228755366759876No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC156600.2226906721333482No Hit
GGTAGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAG156360.22234938374693694No Hit
TCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACA148710.21147081643007798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT144160.2050005574376978No Hit
CCACAAGGGTGCGGCTTACCAAAAATATCAATGCCACAACTTC142950.2032798951562077No Hit
CACCACAAGGGTGCGGCTTACCAAAAATATCAATGCCACAACT140410.19966792640002182No Hit
GCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCT138220.19655366987401907No Hit
GATCTATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGG137540.1955866861125205No Hit
GGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTC137190.19508897388233742No Hit
GGTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGG135540.19274261622576No Hit
ATCCAGTAGCTCTACCTCCGGCAAGAAACACGCAACGCTGCAC130900.18614437408847562No Hit
CCTAAATGCATTTCGGAGAGAACCAGCTATCACGAAGTTTGAT130390.18541913626735168No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGTCAGGA129080.18355627049152354No Hit
GAGTATCCCAAGTAGCACGGGGCCCGAGAAATCCCGTGTGAAT127830.1817787268122982No Hit
ATATAAACCCGTTGTCCATCGACTACGCCTGTCGGCCTCGCCT123990.17631811262971803No Hit
GTGTGACAGCGTGCCTTTTGAAGAATGAGCCTGCGAGTTAGTG123920.1762185701836814No Hit
GACTTACCCAGGGCGGATTAGCCTGGCCCTGGAAACCTTGATC123660.17584884109840254No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG120890.17190980430523922No Hit
GCCATGGATAGATCACTTCGCTTCGGGTCTAGGACATGCGACT119460.16987629433620546No Hit
CTGTCACACCAACAAAGGGTGCTCCAACGGTTTGTAAGCAAAC113270.16107389803668168No Hit
ATGTAACGGGGCTCAAGCACGCCACCGAAGTTGTGGCATTGAT113180.16094591489177745No Hit
GGATAGCCGATGGGGCCCAAAAGCTTACTGACGTTAGCCAAAC110300.15685045425484231No Hit
CCCTTTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTG110050.15649494551899726No Hit
ATCCCAAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGT109800.15613943678315217No Hit
ATTTCACTCCCCTCCCGGGGTACTTTTCACCTTTCCCTCACGG109060.1550871309250508No Hit
GTGGGGGATAAGCTTCATCGTCGAGAGGGAAACAACCCAGACC109020.1550302495273156No Hit
CTTCTAAGCCAACCTCCTGGTTGTCTATGCAACTCCACATCCT108320.1540348250669494No Hit
GTATTTAGGCTTATCAGGTGGTCCTGACAGATTCACACGGGAT108210.15387840122317759No Hit
AATATAAACCCGTTGTCCATCGACTACGCCTGTCGGCCTCGCC108120.15375041807827336No Hit
CGTGTAGCCTCCACGGCTGGTTTACACCGCCGCTTCGCTGGCC107060.15224306103829027No Hit
GAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGGGAGGG105750.15038019526246216No Hit
GCATTTAGGTGCAGCGTTGCGTGTTTCTTGCCGGAGGTAGAGC105440.14993936443001427No Hit
ACCCAGGGCGGATTAGCCTGGCCCTGGAAACCTTGATCTTTCG103660.1474081422307974No Hit
TCTCACTGCTGCGCTCTGACTTACCGGCATTCGGAGTTTGGCT103610.1473370404836284No Hit
CTCCTACCCATCAACACGGCTGAACCAACACCACAAGGGTGCG103500.14718061663985657No Hit
CCATCTAAGGTCCCAAAGCGCGTGCTAAGTGGGAAAGGATGTG103380.14700997244665095No Hit
GGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTACCTGAAAC103140.14666868406023967No Hit
GCAGTGAGACGGTGGGGGATAAGCTTCATCGTCGAGAGGGAAA103050.14654070091533544No Hit
TGTCAGGACCACCTGATAAGCCTAAATACTCCTACATGACCGA103000.14646959916816643No Hit
AGTTAGTGATATGTGGCGAGCTTAACCCGAGAGGGGAATGCGT102070.1451471066708228No Hit
GGCTAAACCGATCATGTGTGATAGCCGGCAGGCGTTGCATGGT101800.14476315723611013No Hit
GTGGGAAAGGATGTGGAGTTGCATAGACAACCAGGAGGTTGGC100760.14328424089499467No Hit
GAGTACGGGAATATAAACCCGTTGTCCATCGACTACGCCTGTC99770.14187642630104821No Hit
ATCCCCCACCGTCTCACTGCTGCGCTCTGACTTACCGGCATTC99660.14172000245727637No Hit
GGTATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCG99620.14166312105954118No Hit
ACCTGAAACCGTTTGCTTACAAACCGTTGGAGCACCCTTTGTT99430.14139293442029893No Hit
CTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCGCG99200.14106586638332147No Hit
CCTATCCACAGCTCATCCCCTCCATTTTTAACTGAAGTGGGTT98980.1407530186957778No Hit
ACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGG98680.14032640821276374No Hit
GACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCAGG98540.1401273233206905No Hit
ACATATCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTG98470.1400277808746539No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT98420.13995667912748486No Hit
CCGTTGGAGCACCCTTTGTTGGTGTGACAGCGTGCCTTTTGAA98410.13994245877805106No Hit
CTTTGGGACCTTAGATGGTGGTCTGGGTTGTTTCCCTCTCGAC98150.1395727296927722No Hit
AAATAGTACCTGAAACCGTTTGCTTACAAACCGTTGGAGCACC97170.13817913544825955No Hit
GAGTGAAATAGTACCTGAAACCGTTTGCTTACAAACCGTTGGA96440.13714104993959195No Hit
ATGTAGGAGTATTTAGGCTTATCAGGTGGTCCTGACAGATTCA96380.13705572784298914No Hit
CTCGTGTAGCCTCCACGGCTGGTTTACACCGCCGCTTCGCTGG96160.1367428801554455No Hit
GGATTGAAAGCCTGGTCATAGAGGGTGCGAACCCCGTAGTCGA95340.13557681150187367No Hit
CCGTTACATTGTCGGCGCGGAATCACTTGACCAGTGAGCTATT95090.13522130276602862No Hit
GGCATGTAGAGCCGAAGAAGGACGTAGTAATCTGCGATAAGCC94670.1346240480898089No Hit
CTACTGGATAGCCGATGGGGCCCAAAAGCTTACTGACGTTAGC93910.13354330153283991No Hit
GTAGTAATCTGCGATAAGCCTCGGGGAGTTGATAAACGAACTG93880.1335006404845385No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG93370.13277540266341456No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA92560.13162355435927656No Hit
GTCCCAAAGCGCGTGCTAAGTGGGAAAGGATGTGGAGTTGCAT89690.12754231407177521No Hit
CGTCTATACAACGTTGTAACGCTCACTGTTCGGCAGAATCAGC89620.1274427716257386No Hit
ACGTTGTAACGCTCACTGTTCGGCAGAATCAGCAGAAAAGTCC89180.12681707625065128No Hit
GTGAAATAGTACCTGAAACCGTTTGCTTACAAACCGTTGGAGC88610.12600651633292453No Hit
GGATTAGCCTGGCCCTGGAAACCTTGATCTTTCGGAGGACGGG88160.12536660060840343No Hit
GGTTAAGCTCGCCACATATCACTAACTCGCAGGCTCATTCTTC87760.12479778663105133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87460.12437117614803725No Hit
TATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACATCTC87440.12434273544916966No Hit
GAGAAATCCCGTGTGAATCTGTCAGGACCACCTGATAAGCCTA87100.12385924356842035No Hit
CTTAGATGGTGGTCTGGGTTGTTTCCCTCTCGACGATGAAGCT87040.12377392147181754No Hit
ACTTGGGATACTCTTCGCGCCATTGCTGCATTTCGACTACGGG86960.12366015867634712No Hit
CCTGATAAGCCTAAATACTCCTACATGACCGATAGTGAACAAG86410.12287803945748797No Hit
GTGATATGTGGCGAGCTTAACCCGAGAGGGGAATGCGTAGCGA85000.12087297018732182No Hit
CTCCTACATGACCGATAGTGAACAAGTACCGTGAGGGAAAGGT84220.11976378293148521No Hit
GGGTAGGAGAGCGTCGTGTGGCCAGCGAAGCGGCGGTGTAAAC83690.11901010441149368No Hit
GTGGAGGACCGAACCCACTTCAGTTAAAAATGGAGGGGATGAG82520.11734632352773879No Hit
ACTCATGCCTGCATTCTCACTCGTGTAGCCTCCACGGCTGGTT81740.11623713627190219No Hit
AAATACTCCTACATGACCGATAGTGAACAAGTACCGTGAGGGA80570.11457335538814727No Hit
GACCGATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTAC80340.11424628735116982No Hit
GGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTCTCTCC80010.11377701581985435No Hit
ACCCAGACCACCATCTAAGGTCCCAAAGCGCGTGCTAAGTGGG79840.1135352698794797No Hit
GCGTTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTC79390.11289535415495858No Hit
GTACTTGTTCACTATCGGTCATGTAGGAGTATTTAGGCTTATC79100.11248296402137829No Hit
CCTAAATACTCCTACATGACCGATAGTGAACAAGTACCGTGAG78400.11148753956101212No Hit
CTACATGACCGATAGTGAACAAGTACCGTGAGGGAAAGGTGAA78040.11097560698139523No Hit
CTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTC78020.11094716628252763No Hit
ACCAGGAGGTTGGCTTAGAAGCAGCCACCCTTGAAAGAGTGCG77930.1108191831376234No Hit
ACGCTCTCCTACCCATCAACACGGCTGAACCAACACCACAAGG77040.10955357203801498No Hit
AAACAATAGTGATTCCGTTAGTAGTGGCGAGCGAACCCGGATG77000.10949669064027975No Hit
AGCTGGTTCTCTCCGAAATGCATTTAGGTGCAGCGTTGCGTGT76600.10892787666292768No Hit
AACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGGGAGGGG76350.10857236792708261No Hit
ATTCTCACTCGTGTAGCCTCCACGGCTGGTTTACACCGCCGCT74750.10629711201767418No Hit
GCTTAGAAGCAGCCACCCTTGAAAGAGTGCGTAATAGCTCACT74590.10606958642673334No Hit
GTACGGGAATATAAACCCGTTGTCCATCGACTACGCCTGTCGG74580.10605536607729954No Hit
CTTGTGGTGTTGGTTCAGCCGTGTTGATGGGTAGGAGAGCGTC74550.10601270502899812No Hit
GTGAATGCCTTGGCATGTAGAGCCGAAGAAGGACGTAGTAATC74070.1053301282561756No Hit
TTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTGCGAG73870.10504572126749956No Hit
GTTGTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTG73680.10477553462825732No Hit
ATTCAGTCGCATGTCCTAGACCCGAAGCGAAGTGATCTATCCA73360.10432048344637564No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC73310.10424938169920663No Hit
ATGCAACTCCACATCCTTTCCCACTTAGCACGCGCTTTGGGAC73250.10416405960260382No Hit
GCGTAATAGCTCACTGGTCAAGTGATTCCGCGCCGACAATGTA73210.1041071782048686No Hit
CTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCT72690.10336772003431086No Hit
GGCCATGGATAGATCACTTCGCTTCGGGTCTAGGACATGCGAC72400.10295532990073059No Hit
GGGATACTCTTCGCGCCATTGCTGCATTTCGACTACGGGGTTC70750.10060897224415316No Hit
ACGTCTTACCGTCGCTTCAACCTGGCCATGGATAGATCACTTC70510.1002676838577419No Hit
GAATATAAACCCGTTGTCCATCGACTACGCCTGTCGGCCTCGC70470.10021080246000669No Hit
GTCAGGACCACCTGATAAGCCTAAATACTCCTACATGACCGAT70410.10012548036340387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGC108300.020.54986237
TCGAAAT113900.019.58823426
TACGCAT66500.018.83383619
CTACGCA66750.018.76329618
AGTCGAA120200.018.68469224
GCTACGC67300.018.60995517
CGCTACG67300.018.55497716
GTCGAAA121100.018.46944825
CGCATTC68100.018.3098421
GCAATGG122500.018.2885736
CAGCAAT120750.018.27784734
ATGCAGC120750.018.23188431
GCAGCAA121400.018.17998333
AGCAATG122750.018.13075435
CTCTATG52350.018.0229231
CGGGTTA70050.017.77373334
TAGACCG5000.017.7600025
TGCAGCA124600.017.74277732
TCGGGTT71050.017.65376533
CCGTAGT127200.017.62735720
Produced by FastQC (version 0.11.5)