FastQCFastQC Report
Thu 26 May 2016
SRR1041751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3756261
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC254520.6775886979099696No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT183480.48846445973802144No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC146710.39057456337565466No Hit
ATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGG141570.376890743215128No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC137990.3673599890955394No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG117520.3128643084173331No Hit
AGGCTAAACCGATCATGTGTGATAGCCGGCAGGCGTTGCATGG113280.30157648789580915No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGT108120.2878394232988602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT105940.2820357797288314No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA103850.27647173612270287No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA101980.2714933813172194No Hit
GGTAGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAG100150.26662151538458057No Hit
GGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTC95610.2545350283167224No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT93290.24835867369173759No Hit
GCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCT92150.24532374081566752No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC90280.24034538601018407No Hit
GAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGCGAAGT86980.23156005400050741No Hit
ATCCACAGCTCATCCCCTCCATTTTTAACTGAAGTGGGTTCGG83870.22328054413684245No Hit
AACAAAGGGTGCTCCAACGGTTTGTAAGCAAACGGTTTCAGGT79990.21295112347091963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79570.21183299030605168No Hit
GATCTATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGG77660.20674814662772367No Hit
GTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTGCGA76730.20427228033408754No Hit
GGTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGG75270.2003854364752609No Hit
GAATATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACAT74900.19940041440144868No Hit
GAGTATCCCAAGTAGCACGGGGCCCGAGAAATCCCGTGTGAAT74850.19926730331039297No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCG74220.19759010356309106No Hit
ATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACATCTCA73200.19487463730555465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69380.18470494994889866No Hit
GGATAGATCACTTCGCTTCGGGTCTAGGACATGCGACTGAATC68330.1819096170367288No Hit
GTGTGACAGCGTGCCTTTTGAAGAATGAGCCTGCGAGTTAGTG65730.17498784030183206No Hit
CTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCGCG65240.17368335160948614No Hit
GTGGGAAAGGATGTGGAGTTGCATAGACAACCAGGAGGTTGGC64120.1707016631698383No Hit
GGATAGCCGATGGGGCCCAAAAGCTTACTGACGTTAGCCAAAC64060.17054192986057146No Hit
GGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTACCTGAAAC63890.17008935215098206No Hit
GGCATGTAGAGCCGAAGAAGGACGTAGTAATCTGCGATAAGCC63360.16867837458579157No Hit
AAATAGTACCTGAAACCGTTTGCTTACAAACCGTTGGAGCACC62800.16718753036596765No Hit
GCAGTGAGACGGTGGGGGATAAGCTTCATCGTCGAGAGGGAAA62320.16590966389183287No Hit
AGTTAGTGATATGTGGCGAGCTTAACCCGAGAGGGGAATGCGT62300.16585641945541058No Hit
ATGTAACGGGGCTCAAGCACGCCACCGAAGTTGTGGCATTGAT61530.1638065086531527No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGTCAGGA60870.16204944225121737No Hit
TCTCAGTACCCACAGGAAGAGAAAACAATAGTGATTCCGTTAG60500.16106442017740513No Hit
TGTCAGGACCACCTGATAAGCCTAAATACTCCTACATGACCGA60120.16005277588538178No Hit
GTGGGGGATAAGCTTCATCGTCGAGAGGGAAACAACCCAGACC58050.15454197671567552No Hit
GAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGGGAGGG57450.15294464362300703No Hit
GTAGTAATCTGCGATAAGCCTCGGGGAGTTGATAAACGAACTG57220.15233233260415077No Hit
GGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTCTCTCC56760.1511077105664383No Hit
TCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACA56460.15030904402010403No Hit
GAGTGAAATAGTACCTGAAACCGTTTGCTTACAAACCGTTGGA55660.14817926656321273No Hit
ACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGG55640.14812602212679044No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA55330.14730073336224506No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCC54760.14578326692421No Hit
TATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGAC52580.1399796233541812No Hit
GTGGAGGACCGAACCCACTTCAGTTAAAAATGGAGGGGATGAG52260.13912771237142468No Hit
AAATACTCCTACATGACCGATAGTGAACAAGTACCGTGAGGGA52200.13896797906215783No Hit
GCATTTAGGTGCAGCGTTGCGTGTTTCTTGCCGGAGGTAGAGC51200.13630575724104368No Hit
GAGAAATCCCGTGTGAATCTGTCAGGACCACCTGATAAGCCTA51000.13577331287682087No Hit
AACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGGGAGGGG49710.13233904672758362No Hit
GGATTGAAAGCCTGGTCATAGAGGGTGCGAACCCCGTAGTCGA49600.13204620232726108No Hit
TTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTGCGAG49180.13092806916239313No Hit
CCTGATAAGCCTAAATACTCCTACATGACCGATAGTGAACAAG48590.1293573582879358No Hit
GTGAATGCCTTGGCATGTAGAGCCGAAGAAGGACGTAGTAATC48550.12925086941509123No Hit
GGTTAAGCTCGCCACATATCACTAACTCGCAGGCTCATTCTTC48320.12863855839623498No Hit
TATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACATCTC47960.12768015854063391No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC47340.12602958101154313No Hit
CACCACAAGGGTGCGGCTTACCAAAAATATCAATGCCACAACT47220.12571011439300941No Hit
AGCTGGTTCTCTCCGAAATGCATTTAGGTGCAGCGTTGCGTGT46940.12496469228309746No Hit
GTGAAATAGTACCTGAAACCGTTTGCTTACAAACCGTTGGAGC46550.12392642577286296No Hit
CCGTTGGAGCACCCTTTGTTGGTGTGACAGCGTGCCTTTTGAA46360.12342060362685128No Hit
GGGATGAGCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGT46290.12323424809937329No Hit
ACCAGGAGGTTGGCTTAGAAGCAGCCACCCTTGAAAGAGTGCG45880.1221427371527165No Hit
ACCTGAAACCGTTTGCTTACAAACCGTTGGAGCACCCTTTGTT45450.12099798176963741No Hit
CTGTCACACCAACAAAGGGTGCTCCAACGGTTTGTAAGCAAAC45440.12097135955142628No Hit
GAGGTAGAGCTACTGGATAGCCGATGGGGCCCAAAAGCTTACT45270.12051878184183686No Hit
CTACTGGATAGCCGATGGGGCCCAAAAGCTTACTGACGTTAGC45010.11982660416834719No Hit
GGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTCTCTCCGA44580.11868184878526811No Hit
ACATATCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTG43980.11708451569259963No Hit
GTGATATGTGGCGAGCTTAACCCGAGAGGGGAATGCGTAGCGA43800.11660531576479909No Hit
GGTCAAGTGATTCCGCGCCGACAATGTAACGGGGCTCAAGCAC43560.1159663825277317No Hit
GGTTTACACCGCCGCTTCGCTGGCCACACGACGCTCTCCTACC43370.11546056038172002No Hit
GGGATACTCTTCGCGCCATTGCTGCATTTCGACTACGGGGTTC43340.11538069372708658No Hit
GGCTAAACCGATCATGTGTGATAGCCGGCAGGCGTTGCATGGT43270.11519433819960859No Hit
GCGTAATAGCTCACTGGTCAAGTGATTCCGCGCCGACAATGTA42160.1122392719781719No Hit
GACCGATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTAC41440.11032247226696974No Hit
ATAGCTCACTGGTCAAGTGATTCCGCGCCGACAATGTAACGGG41300.10994976121201376No Hit
GATGTGGAGTTGCATAGACAACCAGGAGGTTGGCTTAGAAGCA41020.10920433910210178No Hit
ACTTGGGATACTCTTCGCGCCATTGCTGCATTTCGACTACGGG41000.10915109466567953No Hit
CGTGTAGCCTCCACGGCTGGTTTACACCGCCGCTTCGCTGGCC40640.10819269481007843No Hit
TAATAGCTCACTGGTCAAGTGATTCCGCGCCGACAATGTAACG40560.10797971706438929No Hit
TGAATATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACA39820.10600967291676483No Hit
GGTTGAAGCGACGGTAAGACGTCGTGGAGGACCGAACCCACTT39430.10497140640653031No Hit
GCCATGGATAGATCACTTCGCTTCGGGTCTAGGACATGCGACT38760.10318771778638385No Hit
GTTGTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTG38700.103027984477117No Hit
ATGTAGGAGTATTTAGGCTTATCAGGTGGTCCTGACAGATTCA38650.10289487338606129No Hit
TATCAACGCAGAGTACGGGCAAATTTTCAAGAGCAAACGGTGA38520.10254878454931647No Hit
AATATAAACCCGTTGTCCATCGACTACGCCTGTCGGCCTCGCC38480.1024422956764719No Hit
ATTCAGTCGCATGTCCTAGACCCGAAGCGAAGTGATCTATCCA38400.10222931793078276No Hit
AATATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACATC38340.10206958462151593No Hit
AGGTAGAGCTACTGGATAGCCGATGGGGCCCAAAAGCTTACTG37830.10071185149274772No Hit
CTTGTGGTGTTGGTTCAGCCGTGTTGATGGGTAGGAGAGCGTC37660.1002592737831583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAT25750.022.12815719
CTACGCA26050.021.8733218
CGCTACG26800.021.19216316
GCTACGC26950.021.07421117
CGCATTC28050.020.24777221
ACGCATT28750.019.8834820
TATTCCG759.274951E-619.7333345
ATTCCCC30200.019.11258324
CTCTCGG30900.018.6197430
CAATGGC54450.018.5849437
CCCCTCT31250.018.292827
CATTCCC31600.018.20727723
CGCTTTC31900.018.15203910
GCATTCC31550.018.11885822
TCGAAAT55900.018.10286326
TTCCCCT31850.018.06436325
GCTTTCG32350.018.0139111
AGTCGAA58300.017.51629424
TCGGGTT32750.017.45496233
TTCGCTA33200.017.38554214
Produced by FastQC (version 0.11.5)