FastQCFastQC Report
Thu 26 May 2016
SRR1041750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19985570
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1275670.6382955302250574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT794230.3974017253448363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT539530.2699597759783684No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC227010.11358695298657982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA206500.025.3087161
GTATCAA549350.022.9806161
TCGAAAT52350.017.59885426
GTCGAAA53300.017.14634125
TATCAAC770500.016.324662
GTGCGAA59000.015.96017111
ATCAACG797450.015.6616093
CCGTAGT59050.015.50804320
AGTCGAA60450.015.48552624
TCAACGC809000.015.4471574
AACGCAG813200.015.3901256
TGCGAAC59900.015.28797912
CAACGCA818350.015.2797095
CCCGTAG61400.015.06514619
TCGTCTA36700.015.02179927
TAGTCGA62950.014.8999223
CCCCGTA62700.014.72328618
ACGCAGA859200.014.5683197
CGTAGTC64300.014.44323521
CGCAGAG867950.014.4235858
Produced by FastQC (version 0.11.5)