##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041750_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19985570
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58079914658426	34.0	31.0	34.0	31.0	34.0
2	32.823055284387685	34.0	31.0	34.0	31.0	34.0
3	32.88121859921934	34.0	31.0	34.0	31.0	34.0
4	36.34632817577882	37.0	37.0	37.0	35.0	37.0
5	36.26277644320377	37.0	37.0	37.0	35.0	37.0
6	36.357795399380656	37.0	37.0	37.0	35.0	37.0
7	36.329089187849036	37.0	37.0	37.0	35.0	37.0
8	36.31395937168667	37.0	37.0	37.0	35.0	37.0
9	38.076324317995436	39.0	38.0	39.0	35.0	39.0
10	38.024432177816294	39.0	38.0	39.0	35.0	39.0
11	38.079961942541544	39.0	38.0	39.0	35.0	39.0
12	37.9910961258548	39.0	38.0	39.0	35.0	39.0
13	38.00220038757964	39.0	38.0	39.0	35.0	39.0
14	38.89131193155862	40.0	39.0	40.0	36.0	40.0
15	38.88868363524283	40.0	39.0	40.0	36.0	40.0
16	38.88572995416193	40.0	39.0	40.0	36.0	40.0
17	38.88925294600054	40.0	39.0	40.0	36.0	40.0
18	38.86663247533095	40.0	39.0	40.0	36.0	40.0
19	38.89096428072855	40.0	39.0	40.0	36.0	40.0
20	38.86050495432455	40.0	39.0	40.0	36.0	40.0
21	38.829889365176975	40.0	39.0	40.0	36.0	40.0
22	38.77986747438277	40.0	39.0	40.0	36.0	40.0
23	38.7324143369441	40.0	39.0	40.0	36.0	40.0
24	38.70588014252283	40.0	39.0	40.0	36.0	40.0
25	38.66863211807319	40.0	39.0	40.0	36.0	40.0
26	38.57439132333979	40.0	39.0	40.0	35.0	40.0
27	38.515500283454514	40.0	38.0	40.0	35.0	40.0
28	38.45653343887615	40.0	38.0	40.0	35.0	40.0
29	38.38786319329396	40.0	38.0	40.0	35.0	40.0
30	38.31730463529436	40.0	38.0	40.0	35.0	40.0
31	38.23040944041126	40.0	38.0	40.0	35.0	40.0
32	38.1209579211401	40.0	38.0	40.0	34.0	40.0
33	38.06911001287429	40.0	38.0	40.0	34.0	40.0
34	37.98361392744865	40.0	38.0	40.0	34.0	40.0
35	37.914811836740206	40.0	38.0	40.0	34.0	40.0
36	38.009186628152214	40.0	38.0	40.0	34.0	40.0
37	38.01392985038706	40.0	38.0	40.0	34.0	40.0
38	37.98914922116307	40.0	38.0	40.0	34.0	40.0
39	37.94308278422882	40.0	38.0	40.0	34.0	40.0
40	37.91072288656265	40.0	38.0	40.0	34.0	40.0
41	37.842101326106786	40.0	38.0	40.0	34.0	40.0
42	37.764355832733315	40.0	38.0	40.0	34.0	40.0
43	37.655682074616834	40.0	38.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	5.0
14	4.0
15	14.0
16	24.0
17	40.0
18	128.0
19	246.0
20	689.0
21	1474.0
22	3338.0
23	6590.0
24	12562.0
25	21762.0
26	35329.0
27	54891.0
28	82558.0
29	118756.0
30	165004.0
31	222831.0
32	295458.0
33	394524.0
34	551795.0
35	799607.0
36	1281530.0
37	2423420.0
38	9242167.0
39	4270821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68646328325887	19.068643025943217	13.669647650780037	24.575246040017873
2	15.879196840520434	20.933058201492376	39.726382585035104	23.461362372952085
3	18.336199567988302	25.811518010244388	29.803723386423304	26.048559035344
4	13.219117593343595	17.297730312420413	38.094950506790646	31.38820158744534
5	14.081514812937534	36.89376385061822	34.28575217019079	14.738969166253451
6	30.55394967469029	37.785392160443756	17.39835291162574	14.262305253240212
7	27.25485437743332	31.976761233229777	22.195464027295696	18.572920362041213
8	25.651272393031572	35.020562335725224	19.919606996447936	19.408558274795265
9	27.120857698829703	16.000474342237926	19.83323968243087	37.0454282765015
10	16.492679468236332	27.56381729417775	31.54169233101683	24.40181090656909
11	34.07374420644495	24.0456339248768	22.84446227953468	19.036159589143566
12	23.746698242782166	24.70768659587893	29.722584844965645	21.823030316373263
13	28.831927235500416	22.00725323320776	25.616842551901197	23.543976979390628
14	22.92641640943941	20.38436732102212	27.30054234129925	29.38867392823922
15	24.101048906786247	27.618316615438037	24.535457332465374	23.745177145310343
16	23.647391593034374	27.660231857285034	24.812497216741882	23.879879332938717
17	23.275793484999426	26.404570897902836	26.391021121739332	23.928614495358403
18	24.13401269015595	26.028104277236025	27.18418338831467	22.653699644293358
19	25.66969068182694	25.558440414759247	26.909460175516635	21.86240872789718
20	24.619943289083075	25.7546669922349	26.90993551847658	22.71545420020545
21	24.343729000473843	25.66010876847646	27.228255186116783	22.76790704493292
22	23.777425412435072	25.553391772163614	27.097220644695145	23.571962170706165
23	23.47229025742073	25.3329877506621	27.65993664428885	23.534785347628315
24	23.00745487869498	25.722959115001476	27.66569079590925	23.6038952103943
25	23.558672582268105	25.67407384427865	27.505220016241715	23.262033557211527
26	23.352523845954856	25.873852984928625	27.613117864539266	23.160505304577253
27	23.750781188627595	26.18743423379969	26.952736399312105	23.109048178260615
28	23.587668502824787	25.91338650836579	27.08302039921804	23.41592458959139
29	23.76882420666511	26.073762219441328	27.02069543175401	23.136718142139554
30	23.71730203341711	26.28666582939591	27.060008796346562	22.936023340840418
31	23.650623925162005	25.779179678137776	27.31938593695351	23.250810459746706
32	23.573778481174166	26.03409359853134	27.153756435268043	23.238371485026445
33	23.886809332933712	25.82948097052023	27.1572289406807	23.126480755865355
34	23.809013203025984	25.851271692526158	27.016002045475812	23.323713058972046
35	23.87731248095501	26.378917388896088	26.595053330978303	23.1487167991706
36	23.709586466635677	25.90861806793602	27.15571284681898	23.226082618609325
37	24.034761080119306	25.737334486832246	26.828601836224834	23.39930259682361
38	23.75990777345855	25.949667685234896	26.907023417395653	23.3834011239109
39	23.45622866898467	26.07233619056149	27.150549121190938	23.3208860192629
40	24.02447365774406	25.727997750376897	27.214925568797888	23.03260302308115
41	23.966731997135934	25.74625092003881	26.986830998565463	23.300186084259796
42	24.055230849057597	25.698371374947026	26.939827085242	23.30657069075338
43	23.82346362900833	25.383614277701362	27.024843424530797	23.76807866875951
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1261.0
1	2149.0
2	3037.0
3	8460.0
4	13883.0
5	13883.0
6	16039.5
7	18196.0
8	15462.5
9	12729.0
10	18000.5
11	23272.0
12	23272.0
13	37004.0
14	50736.0
15	96774.5
16	142813.0
17	171401.5
18	199990.0
19	199990.0
20	183140.5
21	166291.0
22	174346.5
23	182402.0
24	223555.0
25	264708.0
26	264708.0
27	307486.5
28	350265.0
29	406215.5
30	462166.0
31	529931.5
32	597697.0
33	597697.0
34	675205.0
35	752713.0
36	832781.0
37	912849.0
38	1000998.0
39	1089147.0
40	1089147.0
41	1161666.5
42	1234186.0
43	1324092.0
44	1413998.0
45	1505786.5
46	1597575.0
47	1597575.0
48	1666999.0
49	1736423.0
50	1742055.0
51	1747687.0
52	1705826.0
53	1663965.0
54	1663965.0
55	1566399.0
56	1468833.0
57	1317434.5
58	1166036.0
59	1018526.5
60	871017.0
61	871017.0
62	749734.5
63	628452.0
64	537875.0
65	447298.0
66	365405.5
67	283513.0
68	283513.0
69	231233.5
70	178954.0
71	155414.5
72	131875.0
73	102957.5
74	74040.0
75	74040.0
76	58054.0
77	42068.0
78	33226.0
79	24384.0
80	19727.5
81	15071.0
82	15071.0
83	11837.5
84	8604.0
85	6793.0
86	4982.0
87	3383.5
88	1785.0
89	1785.0
90	1136.5
91	488.0
92	300.5
93	113.0
94	78.5
95	44.0
96	44.0
97	30.5
98	17.0
99	12.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.998557E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.87220367089609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.98909648989392	25.370247059945733
2	16.578122818306213	12.557000877120455
3	6.264420882653451	7.117422706441986
4	2.9662073018693462	4.493472282659802
5	1.702453804212961	3.2237838606722558
6	1.1386387032545313	2.5873654126332375
7	0.788138976601574	2.0893991889978496
8	0.5705237992785098	1.7285594820295334
9	0.41985614410287003	1.4310789661746892
>10	2.288515730748392	15.82212269427504
>50	0.1716161118246022	4.492528925113236
>100	0.10475713332664624	7.573119648036708
>500	0.00944581950939458	2.447248631388981
>1k	0.00747976770870987	5.747471257786508
>5k	5.812133670819754E-4	1.4469677224854625
>10k+	1.453033417704925E-4	1.872211284238855
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	127567	0.6382955302250574	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	79423	0.3974017253448363	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	53953	0.2699597759783684	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	22701	0.11358695298657982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0007220209381069E-5
2	5.003610104690534E-6	0.0	0.0	0.0	1.5010830314071603E-5
3	1.5010830314071603E-5	0.0	0.0	5.003610104690534E-6	1.5010830314071603E-5
4	1.5010830314071603E-5	0.0	0.0	5.003610104690534E-6	1.5010830314071603E-5
5	3.502527073283374E-5	0.0	0.0	1.0007220209381069E-5	1.5010830314071603E-5
6	6.004332125628641E-5	0.0	0.0	1.5010830314071603E-5	2.0014440418762137E-5
7	6.004332125628641E-5	0.0	0.0	1.5010830314071603E-5	2.0014440418762137E-5
8	9.506859198912015E-5	0.0	0.0	2.501805052345267E-5	2.0014440418762137E-5
9	1.1007942230319175E-4	0.0	0.0	4.0028880837524275E-5	2.0014440418762137E-5
10	1.901371839782403E-4	0.0	0.0	6.004332125628641E-5	2.0014440418762137E-5
11	2.351696749204551E-4	0.0	0.0	7.505415157035801E-5	2.0014440418762137E-5
12	2.451768951298362E-4	0.0	0.0	9.506859198912015E-5	2.0014440418762137E-5
13	2.551841153392172E-4	5.003610104690534E-6	0.0	1.0507581219850122E-4	2.0014440418762137E-5
14	2.952129961767415E-4	5.003610104690534E-6	0.0	1.1007942230319175E-4	3.502527073283374E-5
15	3.052202163861226E-4	1.0007220209381069E-5	0.0	1.1508303240788228E-4	5.5039711151595876E-5
16	3.352418770142658E-4	1.0007220209381069E-5	0.0	1.2008664251257282E-4	6.004332125628641E-5
17	3.352418770142658E-4	1.0007220209381069E-5	0.0	1.300938627219539E-4	6.004332125628641E-5
18	3.452490972236469E-4	1.5010830314071603E-5	0.0	1.5010830314071601E-4	7.005054146566748E-5
19	3.752707578517901E-4	1.5010830314071603E-5	0.0	1.7012274355947816E-4	7.005054146566748E-5
20	4.903537902596724E-4	2.501805052345267E-5	0.0	1.8012996376885924E-4	7.005054146566748E-5
21	6.65480143923841E-4	2.501805052345267E-5	0.0	2.1515523450169296E-4	8.506137177973908E-5
22	6.804909742379126E-4	2.501805052345267E-5	0.0	2.651913355485983E-4	9.006498188442962E-5
23	6.804909742379126E-4	2.501805052345267E-5	0.0	3.102238264908131E-4	1.5010830314071601E-4
24	6.955018045519842E-4	2.501805052345267E-5	0.0	3.702671477470995E-4	1.5511191324540656E-4
25	7.155162449707464E-4	3.0021660628143206E-5	0.0	4.102960285846238E-4	1.5511191324540656E-4
26	7.95574006645795E-4	3.0021660628143206E-5	0.0	4.853501801549818E-4	1.7012274355947816E-4
27	8.105848369598666E-4	3.502527073283374E-5	0.0	7.30527075284818E-4	1.7012274355947816E-4
28	8.356028874833192E-4	3.502527073283374E-5	0.0	0.002371711189623313	1.7012274355947816E-4
29	8.406064975880098E-4	3.502527073283374E-5	0.0	0.006139429598455285	1.751263536641687E-4
30	8.506137177973908E-4	3.502527073283374E-5	0.0	0.0110479711111567	1.8012996376885924E-4
31	9.006498188442962E-4	3.502527073283374E-5	0.0	0.02163561009268187	1.901371839782403E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20650	0.0	25.308716	1
GTATCAA	54935	0.0	22.980616	1
TCGAAAT	5235	0.0	17.598854	26
GTCGAAA	5330	0.0	17.146341	25
TATCAAC	77050	0.0	16.32466	2
GTGCGAA	5900	0.0	15.960171	11
ATCAACG	79745	0.0	15.661609	3
CCGTAGT	5905	0.0	15.508043	20
AGTCGAA	6045	0.0	15.485526	24
TCAACGC	80900	0.0	15.447157	4
AACGCAG	81320	0.0	15.390125	6
TGCGAAC	5990	0.0	15.287979	12
CAACGCA	81835	0.0	15.279709	5
CCCGTAG	6140	0.0	15.065146	19
TCGTCTA	3670	0.0	15.021799	27
TAGTCGA	6295	0.0	14.89992	23
CCCCGTA	6270	0.0	14.723286	18
ACGCAGA	85920	0.0	14.568319	7
CGTAGTC	6430	0.0	14.443235	21
CGCAGAG	86795	0.0	14.423585	8
>>END_MODULE