FastQCFastQC Report
Thu 26 May 2016
SRR1041749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15865449
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT933700.5885115511070629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT627310.395393789359507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT403440.2542884225968014No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC305170.19234879517119247No Hit
CTCTATGACCAGGCTTTCAATCCTGTTCGTCTATACAACGTTG203440.12822832811097878No Hit
GTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTGCGA161540.10181873831619893No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC158970.10019886610205611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174300.024.6560521
GTATCAA442550.022.1097051
TCGAAAT65600.018.2743926
TCGTCTA45200.017.8860627
GTCGAAA68550.017.56892825
GTTCGTC47350.016.83949325
CCGTAGT73450.016.34649320
AGTCGAA75450.016.33001924
CCCGTAG75300.016.11686719
CGTCTAT50350.016.05660428
GTGCGAA75750.016.04554611
TTCGTCT50050.015.85714226
TGCGAAC76350.015.74983712
TGTTCGT51550.015.68283324
TAGTCGA79500.015.567924523
CCCCGTA77650.015.53380618
TATCAAC630250.015.4633872
CGGGTTA42600.015.460093534
GTAGTCG79000.015.31518922
CGTAGTC79250.015.24353321
Produced by FastQC (version 0.11.5)