##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041749_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15865449
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31126651379359	33.0	31.0	34.0	31.0	34.0
2	32.57683485667503	34.0	31.0	34.0	31.0	34.0
3	32.64209925606266	34.0	31.0	34.0	31.0	34.0
4	36.1926539236299	37.0	35.0	37.0	35.0	37.0
5	36.08067026656479	37.0	35.0	37.0	35.0	37.0
6	36.18319015112652	37.0	36.0	37.0	35.0	37.0
7	36.1442670799925	37.0	36.0	37.0	35.0	37.0
8	36.1285501595322	37.0	36.0	37.0	35.0	37.0
9	37.84053706894775	39.0	38.0	39.0	35.0	39.0
10	37.76832184201027	39.0	37.0	39.0	35.0	39.0
11	37.83076949161666	39.0	38.0	39.0	35.0	39.0
12	37.7232402940503	39.0	37.0	39.0	35.0	39.0
13	37.737992854787784	39.0	37.0	39.0	35.0	39.0
14	38.613093395591896	40.0	38.0	40.0	36.0	40.0
15	38.60944105647435	40.0	38.0	40.0	36.0	40.0
16	38.60502895316735	40.0	38.0	40.0	36.0	40.0
17	38.6033235491791	40.0	38.0	40.0	36.0	40.0
18	38.574104521088564	40.0	38.0	40.0	36.0	40.0
19	38.598225678958094	40.0	39.0	40.0	36.0	40.0
20	38.56191091723909	40.0	38.0	40.0	36.0	40.0
21	38.52582174005917	40.0	38.0	40.0	35.0	40.0
22	38.47123879065761	40.0	38.0	40.0	35.0	40.0
23	38.42031788700086	40.0	38.0	40.0	35.0	40.0
24	38.385814671869674	40.0	38.0	40.0	35.0	40.0
25	38.3474976346399	40.0	38.0	40.0	35.0	40.0
26	38.22996720735732	40.0	38.0	40.0	34.0	40.0
27	38.16522450767072	40.0	38.0	40.0	34.0	40.0
28	38.10180985107954	40.0	38.0	40.0	34.0	40.0
29	38.01973521203213	40.0	38.0	40.0	34.0	40.0
30	37.94268192472838	40.0	38.0	40.0	34.0	40.0
31	37.84827873450036	40.0	38.0	40.0	34.0	40.0
32	37.728946467257245	40.0	38.0	40.0	33.0	40.0
33	37.67370460174181	40.0	37.0	40.0	33.0	40.0
34	37.5697747980533	40.0	37.0	40.0	33.0	40.0
35	37.49845068992374	40.0	37.0	40.0	33.0	40.0
36	37.59700459785286	40.0	37.0	40.0	33.0	40.0
37	37.60837868502808	40.0	37.0	40.0	33.0	40.0
38	37.582037041624226	40.0	37.0	40.0	33.0	40.0
39	37.52510565569244	40.0	37.0	40.0	33.0	40.0
40	37.48945075553802	40.0	37.0	40.0	33.0	40.0
41	37.41466686508525	40.0	37.0	40.0	33.0	40.0
42	37.32711195252022	40.0	37.0	40.0	33.0	40.0
43	37.2135523551839	40.0	37.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	0.0
13	5.0
14	6.0
15	7.0
16	15.0
17	45.0
18	131.0
19	285.0
20	684.0
21	1755.0
22	3655.0
23	7669.0
24	13828.0
25	24025.0
26	39247.0
27	60372.0
28	89454.0
29	128559.0
30	176670.0
31	236347.0
32	308633.0
33	405619.0
34	553782.0
35	781961.0
36	1210061.0
37	2180198.0
38	7196413.0
39	2446020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.439580499738774	19.37435240565836	13.475332466165943	24.710734628436924
2	16.39177687312852	20.963592016841123	38.86373464753503	23.780896462495328
3	18.419869491244782	25.66812953103313	29.770402337809664	26.141598639912427
4	13.58324621005053	17.263488729502708	37.52028700858072	31.63297805186604
5	14.254264092998566	36.85968799244194	34.02101636077239	14.8650315537871
6	30.865864558891467	37.650406238108985	17.04250538386906	14.441223819130489
7	27.501408879130995	31.725228829010767	21.92719537909075	18.846166912767487
8	26.016124724865964	34.76547685476787	19.51020736948573	19.708191050880437
9	27.382811542238734	15.63364516188606	19.630809061880317	37.35273423399489
10	17.056895143654614	27.04435279455375	30.943712970241183	24.95503909155045
11	34.55818363539538	23.787974736800706	22.41659848391306	19.237243143890854
12	23.862873341939455	24.397676989790835	29.30779960907504	22.43165005919467
13	29.078155934950217	21.623957821805107	25.604362032237475	23.6935242110072
14	23.175467646708263	20.082614743522228	26.965867779726878	29.776049830042627
15	24.692172279523888	27.276019733195074	24.114930500863856	23.91687748641718
16	24.129471532762796	27.305977914649628	24.228750160175107	24.33580039241247
17	23.636349655153158	26.11731316270973	26.152773867288598	24.093563314848513
18	24.57508136076073	25.644720171487112	26.80304225868426	22.977156209067893
19	26.177740069001516	25.226799443242985	26.43035189234165	22.165108595413844
20	24.93370972356345	25.611497033585373	26.24779796651201	23.20699527633917
21	24.886348946065127	25.352140995190243	26.585922654946607	23.175587403798026
22	24.178464788484714	25.22927652409963	26.5273551350485	24.06490355236716
23	23.952527281137773	24.90558571648366	27.360063998188767	23.781823004189796
24	23.386189700650768	25.526337136755473	27.238056735740667	23.849416426853097
25	23.930964701975974	25.563487046600446	27.112765607831207	23.392782643592376
26	23.691248826301734	25.61495738317901	27.2553206656805	23.43847312483876
27	24.137659135899654	26.034913981949074	26.44150190769892	23.385924974452347
28	24.156171060774895	25.429441045128947	26.63086938163553	23.78351851246063
29	24.30555857574532	25.569241689913724	26.446550614483083	23.678649119857877
30	23.854250831476627	26.21389410410005	26.612710425024844	23.31914463939848
31	23.890965833995622	25.477274547981594	26.949643845566552	23.682115772456235
32	23.995627227442476	25.617592039153763	26.652696686995746	23.734084046408015
33	24.54063543994248	25.724018273923416	26.64497550620849	23.09037077992561
34	24.387201395939062	25.426648814036085	26.291912696577324	23.89423709344753
35	24.308810926183053	26.440928334269014	25.865344245851475	23.38491649369646
36	24.186778451716055	25.566966305208254	26.67589174438114	23.57036349869455
37	24.660411438718185	25.223200427545418	26.21830620740705	23.898081926329347
38	24.17925266407525	25.63492530214556	26.353984687102145	23.831837346677045
39	23.83840507759976	25.543077917303194	26.77849205528315	23.840024949813902
40	24.409268215478804	25.465134960882608	26.88962663458185	23.235970189056737
41	24.62622394109363	25.218662264143926	26.474460319402244	23.6806534753602
42	24.654284918126173	25.24872129367407	26.40921161449638	23.687782173703372
43	24.279817104451315	24.88199356980064	26.619202519890866	24.21898680585718
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1067.0
1	1841.0
2	2615.0
3	7066.0
4	11517.0
5	11517.0
6	13149.5
7	14782.0
8	12362.0
9	9942.0
10	13826.5
11	17711.0
12	17711.0
13	27998.5
14	38286.0
15	73891.0
16	109496.0
17	125760.0
18	142024.0
19	142024.0
20	127772.0
21	113520.0
22	118381.5
23	123243.0
24	150122.0
25	177001.0
26	177001.0
27	204908.0
28	232815.0
29	271157.5
30	309500.0
31	358285.5
32	407071.0
33	407071.0
34	465069.5
35	523068.0
36	584359.0
37	645650.0
38	721610.5
39	797571.0
40	797571.0
41	861869.0
42	926167.0
43	1010690.5
44	1095214.0
45	1189848.0
46	1284482.0
47	1284482.0
48	1369324.0
49	1454166.0
50	1470750.0
51	1487334.0
52	1468235.5
53	1449137.0
54	1449137.0
55	1373599.0
56	1298061.0
57	1148747.0
58	999433.0
59	857965.5
60	716498.0
61	716498.0
62	616840.5
63	517183.0
64	445469.5
65	373756.0
66	298909.5
67	224063.0
68	224063.0
69	181978.5
70	139894.0
71	118399.5
72	96905.0
73	76580.0
74	56255.0
75	56255.0
76	43078.0
77	29901.0
78	23798.0
79	17695.0
80	14375.5
81	11056.0
82	11056.0
83	8583.0
84	6110.0
85	4797.5
86	3485.0
87	2400.0
88	1315.0
89	1315.0
90	822.5
91	330.0
92	208.0
93	86.0
94	58.5
95	31.0
96	31.0
97	18.0
98	5.0
99	6.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.5865449E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.859534235832687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.49301445294499	20.547174019356532
2	16.77804814345758	10.690815984738714
3	6.6072206163957565	6.3150891429528055
4	3.364273312311198	4.287367230891073
5	2.0734354969492252	3.3029344600422306
6	1.3288966705485004	2.5402817382734413
7	0.9285800334889085	2.070888915735538
8	0.6968902920073763	1.7762080093462755
9	0.5191172046153841	1.4884949117568234
>10	2.842109692551165	16.584065533192938
>50	0.21102830470364217	4.639683982976783
>100	0.12890859934962667	7.8378577736590405
>500	0.013761112044032296	3.020498749792226
>1k	0.012619467662842982	8.526307087794335
>5k	0.0016812366268688106	3.5175599329581972
>10k+	4.15364343108765E-4	2.8547725265331563
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	93370	0.5885115511070629	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	62731	0.395393789359507	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40344	0.2542884225968014	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	30517	0.19234879517119247	No Hit
CTCTATGACCAGGCTTTCAATCCTGTTCGTCTATACAACGTTG	20344	0.12822832811097878	No Hit
GTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTGCGA	16154	0.10181873831619893	No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC	15897	0.10019886610205611	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.2606009448582263E-5	0.0	0.0	0.0	0.0
3	1.8909014172873393E-5	0.0	0.0	0.0	0.0
4	2.5212018897164526E-5	0.0	0.0	0.0	0.0
5	5.042403779432905E-5	0.0	0.0	0.0	0.0
6	9.454507086436697E-5	0.0	0.0	0.0	1.8909014172873393E-5
7	9.454507086436697E-5	6.303004724291131E-6	0.0	0.0	1.8909014172873393E-5
8	1.1975708976153149E-4	6.303004724291131E-6	0.0	6.303004724291131E-6	2.5212018897164526E-5
9	1.3236309921011376E-4	6.303004724291131E-6	0.0	1.2606009448582263E-5	5.042403779432905E-5
10	2.269081700744807E-4	1.2606009448582263E-5	0.0	1.2606009448582263E-5	5.042403779432905E-5
11	3.781802834574679E-4	1.2606009448582263E-5	0.0	2.5212018897164526E-5	5.672704251862018E-5
12	3.9708929763034126E-4	1.2606009448582263E-5	0.0	4.412103307003792E-5	5.672704251862018E-5
13	4.096953070789235E-4	1.8909014172873393E-5	0.0	5.672704251862018E-5	6.933305196720244E-5
14	4.7902835904612594E-4	1.8909014172873393E-5	0.0	6.933305196720244E-5	9.454507086436697E-5
15	5.105433826675816E-4	1.8909014172873393E-5	0.0	7.563605669149357E-5	1.0715108031294922E-4
16	5.29452396840455E-4	2.5212018897164526E-5	0.0	8.824206614007584E-5	1.1345408503724035E-4
17	5.357554015647461E-4	2.5212018897164526E-5	0.0	8.824206614007584E-5	1.1345408503724035E-4
18	5.420584062890372E-4	2.5212018897164526E-5	0.0	8.824206614007584E-5	1.2606009448582262E-4
19	5.672704251862018E-4	2.5212018897164526E-5	0.0	1.0715108031294922E-4	1.3236309921011376E-4
20	6.429064818776954E-4	3.1515023621455654E-5	0.0	1.3236309921011376E-4	1.3236309921011376E-4
21	9.13935685022214E-4	3.1515023621455654E-5	0.0	1.8909014172873394E-4	1.44969108658696E-4
22	9.265416944707962E-4	3.1515023621455654E-5	0.0	2.647261984202275E-4	1.44969108658696E-4
23	9.328446991950874E-4	3.1515023621455654E-5	0.0	3.3405925038742997E-4	2.3951417952306297E-4
24	9.580567180922519E-4	3.1515023621455654E-5	0.0	4.6011934487325256E-4	2.584231936959364E-4
25	9.706627275408342E-4	3.781802834574679E-5	0.0	5.042403779432905E-4	2.647261984202275E-4
26	0.0010462987842323277	3.781802834574679E-5	0.0	6.303004724291131E-4	2.710292031445186E-4
27	0.0010778138078537834	3.781802834574679E-5	0.0	9.13935685022214E-4	2.7733220786880976E-4
28	0.001103025826750948	3.781802834574679E-5	0.0	0.002685080012548022	2.836352125931009E-4
29	0.001103025826750948	3.781802834574679E-5	0.0	0.006756821064440093	2.836352125931009E-4
30	0.001109328831475239	3.781802834574679E-5	0.0	0.012549282406063641	2.836352125931009E-4
31	0.0011408438550966947	3.781802834574679E-5	0.0	0.02369299475861036	2.836352125931009E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17430	0.0	24.656052	1
GTATCAA	44255	0.0	22.109705	1
TCGAAAT	6560	0.0	18.27439	26
TCGTCTA	4520	0.0	17.88606	27
GTCGAAA	6855	0.0	17.568928	25
GTTCGTC	4735	0.0	16.839493	25
CCGTAGT	7345	0.0	16.346493	20
AGTCGAA	7545	0.0	16.330019	24
CCCGTAG	7530	0.0	16.116867	19
CGTCTAT	5035	0.0	16.056604	28
GTGCGAA	7575	0.0	16.045546	11
TTCGTCT	5005	0.0	15.857142	26
TGCGAAC	7635	0.0	15.749837	12
TGTTCGT	5155	0.0	15.682833	24
TAGTCGA	7950	0.0	15.5679245	23
CCCCGTA	7765	0.0	15.533806	18
TATCAAC	63025	0.0	15.463387	2
CGGGTTA	4260	0.0	15.4600935	34
GTAGTCG	7900	0.0	15.315189	22
CGTAGTC	7925	0.0	15.243533	21
>>END_MODULE