FastQCFastQC Report
Thu 26 May 2016
SRR1041748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10339798
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC529190.5117991666761769No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT338220.32710503628794296No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC282340.27306142731221633No Hit
GTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTGCGA277260.26814837195078667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT269900.26103024449800666No Hit
CTCTATGACCAGGCTTTCAATCCTGTTCGTCTATACAACGTTG248340.24017877331839557No Hit
GAATATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACAT238000.23017857795674537No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC230300.22273162396402715No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA221600.2143175330891377No Hit
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTA208710.20185113867795096No Hit
ATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACATCTCA200230.1936498179171392No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA199230.192682681034968No Hit
ATCCACAGCTCATCCCCTCCATTTTTAACTGAAGTGGGTTCGG198990.1924505681832469No Hit
GTATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGA198300.19178324373454878No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCG193030.18668643236550655No Hit
ATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGG190620.18435563247947395No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT187890.1817153487911466No Hit
GCCTAAATACTCCTACATGACCGATAGTGAACAAGTACCGTGA182300.17630905361980959No Hit
TATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGAC178290.17243083472230308No Hit
GAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGCGAAGT170800.1651869794748408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT164190.15879420468368918No Hit
GGATAGATCACTTCGCTTCGGGTCTAGGACATGCGACTGAATC163050.157691668638014No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGT162460.15712105787753303No Hit
TCTCAGTACCCACAGGAAGAGAAAACAATAGTGATTCCGTTAG160300.15503204221204323No Hit
AACAAAGGGTGCTCCAACGGTTTGTAAGCAAACGGTTTCAGGT156600.15145363574800977No Hit
GGTAGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAG154810.14972246072892334No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCC154100.14903579354258178No Hit
AGGCTAAACCGATCATGTGTGATAGCCGGCAGGCGTTGCATGG152220.14721757620409995No Hit
TCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACA148580.14369719795299676No Hit
GGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTC142330.13765259243942676No Hit
GATCTATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGG139740.13514770791460337No Hit
GGTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGG132420.12806826593711018No Hit
GGTTTACACCGCCGCTTCGCTGGCCACACGACGCTCTCCTACC130520.1262307058609849No Hit
CCCTTTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTG129980.12570845194461247No Hit
GCATTTAGGTGCAGCGTTGCGTGTTTCTTGCCGGAGGTAGAGC126640.12247821475816065No Hit
CCTAAATGCATTTCGGAGAGAACCAGCTATCACGAAGTTTGAT126270.1221203741117573No Hit
GCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCT125890.12175286209653226No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC123900.11982825970101157No Hit
GTATTTAGGCTTATCAGGTGGTCCTGACAGATTCACACGGGAT122480.11845492532832845No Hit
ATCCAGTAGCTCTACCTCCGGCAAGAAACACGCAACGCTGCAC120310.11635623829401695No Hit
CCACAAGGGTGCGGCTTACCAAAAATATCAATGCCACAACTTC120170.11622083913051298No Hit
GGATAGCCGATGGGGCCCAAAAGCTTACTGACGTTAGCCAAAC119380.11545680099359776No Hit
GTGTGACAGCGTGCCTTTTGAAGAATGAGCCTGCGAGTTAGTG117160.1133097571151777No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGTCAGGA116130.11231360612654136No Hit
CACCACAAGGGTGCGGCTTACCAAAAATATCAATGCCACAACT114120.11036966099337725No Hit
ATTTCACTCCCCTCCCGGGGTACTTTTCACCTTTCCCTCACGG113570.10983773570818309No Hit
GAGTATCCCAAGTAGCACGGGGCCCGAGAAATCCCGTGTGAAT112160.10847407270432169No Hit
GGATTGAAAGCCTGGTCATAGAGGGTGCGAACCCCGTAGTCGA109440.10584346038481603No Hit
CCATCTAAGGTCCCAAAGCGCGTGCTAAGTGGGAAAGGATGTG108550.10498270855968367No Hit
ATGTAACGGGGCTCAAGCACGCCACCGAAGTTGTGGCATTGAT105940.10245848129721684No Hit
ATATAAACCCGTTGTCCATCGACTACGCCTGTCGGCCTCGCCT105400.10193622738084437No Hit
GGTATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCG103480.10007932456707568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83800.029.7810251
GTATCAA216750.022.464591
TCGAAAT110100.018.75204326
CAATGGC112750.018.47538837
CTACGCA59850.017.80451218
TACGCAT59650.017.77116619
GTCGAAA118000.017.49660925
AGTCGAA119950.017.33555624
CGCTACG61500.017.20650316
GCTACGC62350.017.03127517
TCGTCTA57000.016.87719227
GCAATGG124850.016.67000436
ACGCATT64450.016.56245220
CGCATTC64650.016.42536721
AGCAATG127800.016.32863835
CCCGTAG127800.016.24178319
CGGGTTA66050.016.2172634
GCAGCAA127900.016.15676333
ATGCAGC128250.016.15594531
CCGTAGT128100.016.13153620
Produced by FastQC (version 0.11.5)