##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041748_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10339798
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.680405845452686	31.0	31.0	34.0	30.0	34.0
2	31.976962025757178	31.0	31.0	34.0	30.0	34.0
3	32.046959041172755	33.0	31.0	34.0	30.0	34.0
4	35.746269414547555	37.0	35.0	37.0	33.0	37.0
5	35.57341787528151	37.0	35.0	37.0	33.0	37.0
6	35.745010879322784	37.0	35.0	37.0	33.0	37.0
7	35.7101366970612	37.0	35.0	37.0	33.0	37.0
8	35.697780943109336	37.0	35.0	37.0	33.0	37.0
9	37.322939964591185	39.0	37.0	39.0	34.0	39.0
10	37.15124695859629	39.0	37.0	39.0	33.0	39.0
11	37.258659791999804	39.0	37.0	39.0	34.0	39.0
12	37.07468124619069	39.0	37.0	39.0	33.0	39.0
13	37.119264902467144	39.0	37.0	39.0	33.0	39.0
14	37.95710573842932	40.0	38.0	40.0	33.0	40.0
15	37.95669151370269	40.0	38.0	40.0	33.0	40.0
16	37.92910345057031	40.0	38.0	40.0	33.0	40.0
17	37.90451834745708	40.0	38.0	40.0	33.0	40.0
18	37.85528769517548	40.0	38.0	40.0	33.0	40.0
19	37.85812295365925	40.0	38.0	40.0	33.0	40.0
20	37.81257554547971	40.0	37.0	40.0	33.0	40.0
21	37.758914148999814	40.0	37.0	40.0	33.0	40.0
22	37.69086117543109	40.0	37.0	40.0	33.0	40.0
23	37.62305549876313	40.0	37.0	40.0	33.0	40.0
24	37.549476401763364	40.0	37.0	40.0	33.0	40.0
25	37.529167107519896	40.0	37.0	40.0	33.0	40.0
26	37.33143074942083	39.0	37.0	40.0	32.0	40.0
27	37.2696413411558	39.0	37.0	40.0	32.0	40.0
28	37.17877767051155	39.0	37.0	40.0	32.0	40.0
29	37.0579524861124	39.0	36.0	40.0	31.0	40.0
30	36.96719974606854	39.0	36.0	40.0	31.0	40.0
31	36.86347054362184	39.0	36.0	40.0	31.0	40.0
32	36.7135767062374	39.0	36.0	40.0	31.0	40.0
33	36.63615227299412	39.0	36.0	40.0	30.0	40.0
34	36.482880613335	38.0	35.0	40.0	30.0	40.0
35	36.4095200892706	38.0	35.0	40.0	30.0	40.0
36	36.51088251433926	39.0	35.0	40.0	30.0	40.0
37	36.566057383326054	39.0	35.0	40.0	30.0	40.0
38	36.54101211648429	39.0	35.0	40.0	30.0	40.0
39	36.43693580861058	39.0	35.0	40.0	30.0	40.0
40	36.40181220174708	39.0	35.0	40.0	30.0	40.0
41	36.30012346469438	38.0	35.0	40.0	30.0	40.0
42	36.19045323709419	38.0	35.0	40.0	30.0	40.0
43	36.06153698553879	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	2.0
14	2.0
15	6.0
16	18.0
17	47.0
18	153.0
19	429.0
20	1079.0
21	2555.0
22	5444.0
23	10642.0
24	18870.0
25	31419.0
26	49424.0
27	72602.0
28	103640.0
29	141625.0
30	187150.0
31	242449.0
32	310394.0
33	400408.0
34	531314.0
35	734835.0
36	1116604.0
37	1942476.0
38	4007436.0
39	428770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90489001816089	21.690220640674024	13.884323465506773	23.520565875658306
2	18.56205508076657	22.021987276734034	36.602233428544736	22.813724213954664
3	20.193112089810654	25.755493482561263	29.686566410678427	24.364828016949655
4	15.514113525235212	18.689427008148517	36.34192853670836	29.454530929907914
5	14.601939032077802	37.719479626197725	33.281955798362794	14.39662554336168
6	30.854742036546558	38.22947024690425	16.585681847943256	14.330105868605944
7	27.627087105570148	31.759566289399466	21.039385875816915	19.57396072921347
8	26.417276236924554	34.72361839177129	18.30734024010914	20.55176513119502
9	28.039745070454952	15.138023005865298	19.243306300567962	37.578925623111786
10	18.298442580793164	26.218278152049006	29.58443675592115	25.89884251123668
11	36.26362913472778	23.452334368621127	21.1540786386736	19.129957857977498
12	24.095432038420867	23.67553988965742	28.47542089313544	23.75360717878628
13	29.58398220158653	21.220433900159367	25.43860141174905	23.756982486505056
14	23.569038776192727	19.863743953218428	26.344209045476518	30.22300822511233
15	26.443572688750788	26.61931113161011	23.00350548434312	23.933610695295982
16	25.276112744175467	27.36551526441813	22.615441810371927	24.742930181034485
17	24.779313870541763	26.00141704895976	25.34949909079462	23.869769989703858
18	25.527674718597016	25.340543403265713	25.489318069850107	23.642463808287165
19	27.49709423723752	24.824653247577952	25.21190452656812	22.466347988616413
20	25.596467165025853	26.110877601283892	24.392836301057333	23.89981893263292
21	26.253365878134176	25.296325905012846	24.712426683770804	23.737881533082174
22	24.796422521987374	25.569571088332673	25.028390303176135	24.60561608650382
23	24.810716805105866	24.6074343038423	26.800378498690208	23.78147039236163
24	23.64671921056872	25.875099300779375	26.580644999060908	23.897536489590994
25	24.38162718459297	26.20705936421582	26.38273977886222	23.028573672328996
26	24.135316763441608	25.97329270842622	26.481755252858907	23.40963527527327
27	25.094426409490783	26.52058579867808	24.708200295595717	23.67678749623542
28	25.44723794410684	25.36159797319058	25.150278564436174	24.04088551826641
29	25.46689016555256	25.11157374641168	24.868745018036137	24.55279106999963
30	23.783414337494794	27.169544317983775	25.135616769302455	23.911424575218973
31	24.20707832009871	25.866395068839836	25.65926336278523	24.267263248276226
32	25.078923205269582	25.43860141174905	25.361095062011852	24.12138032096952
33	26.43799230894066	25.96339889812161	25.21891626896386	22.379692523973873
34	25.659408433317555	25.278530586380892	24.296925336452414	24.765135643849135
35	25.523448330421928	27.60044248446633	23.659011520341114	23.217097664770627
36	25.186613897099342	25.601051393847346	25.663799234762614	23.548535474290695
37	26.44897898392212	24.562056241330826	24.41564138873893	24.573323386008123
38	25.03580824306239	25.49102990213155	25.100616085536682	24.37254576926938
39	24.435312952922292	24.865833936020802	26.169186283909994	24.52966682714691
40	25.054415956675363	25.433262816159463	26.083623683944307	23.428697543220864
41	26.282438012812243	24.575712214107085	25.18817098747964	23.953678785601035
42	26.444887994910538	24.685656334872306	24.831181421532605	24.038274248684548
43	25.299130601971143	24.073671458572015	25.470787727187705	25.156410212269137
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	506.0
1	734.0
2	962.0
3	2178.0
4	3394.0
5	3394.0
6	4289.0
7	5184.0
8	4360.0
9	3536.0
10	5089.5
11	6643.0
12	6643.0
13	10980.5
14	15318.0
15	30977.5
16	46637.0
17	51652.0
18	56667.0
19	56667.0
20	54730.0
21	52793.0
22	56655.0
23	60517.0
24	73253.5
25	85990.0
26	85990.0
27	99715.0
28	113440.0
29	131131.5
30	148823.0
31	173132.0
32	197441.0
33	197441.0
34	224531.0
35	251621.0
36	280950.5
37	310280.0
38	369158.5
39	428037.0
40	428037.0
41	476330.5
42	524624.0
43	600302.5
44	675981.0
45	785928.5
46	895876.0
47	895876.0
48	1011771.0
49	1127666.0
50	1152561.0
51	1177456.0
52	1177949.5
53	1178443.0
54	1178443.0
55	1122884.0
56	1067325.0
57	879915.0
58	692505.0
59	554196.5
60	415888.0
61	415888.0
62	348980.0
63	282072.0
64	261176.0
65	240280.0
66	179001.5
67	117723.0
68	117723.0
69	90630.5
70	63538.0
71	53398.0
72	43258.0
73	33080.5
74	22903.0
75	22903.0
76	17427.5
77	11952.0
78	9245.5
79	6539.0
80	5201.0
81	3863.0
82	3863.0
83	3057.0
84	2251.0
85	1731.5
86	1212.0
87	838.0
88	464.0
89	464.0
90	300.5
91	137.0
92	88.0
93	39.0
94	25.5
95	12.0
96	12.0
97	7.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.0339798E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.007992991305816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.82902843326582	21.854529747890524
2	14.002973040606994	8.404022337199578
3	4.918997305927419	4.428277100415665
4	2.4864328229261807	2.984514348948862
5	1.4039684547558349	2.1065137775163776
6	0.8886160000484686	1.599934962084999
7	0.6098365106798123	1.280997881682554
8	0.4696525701168158	1.1274664825931338
9	0.3472228646436487	0.9377515159782964
>10	1.8032266671409816	9.6545762189791
>50	0.11693535781151787	2.4029244084113963
>100	0.07882520553517286	4.8547035074506155
>500	0.013871381940600508	2.9649969349742338
>1k	0.023847955550044195	16.017357535309447
>5k	0.0047631544094990565	10.368985418294887
>10k+	0.0018022746414320754	9.012447822270492
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	52919	0.5117991666761769	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	33822	0.32710503628794296	No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC	28234	0.27306142731221633	No Hit
GTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTGCGA	27726	0.26814837195078667	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26990	0.26103024449800666	No Hit
CTCTATGACCAGGCTTTCAATCCTGTTCGTCTATACAACGTTG	24834	0.24017877331839557	No Hit
GAATATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACAT	23800	0.23017857795674537	No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC	23030	0.22273162396402715	No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA	22160	0.2143175330891377	No Hit
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTA	20871	0.20185113867795096	No Hit
ATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACATCTCA	20023	0.1936498179171392	No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA	19923	0.192682681034968	No Hit
ATCCACAGCTCATCCCCTCCATTTTTAACTGAAGTGGGTTCGG	19899	0.1924505681832469	No Hit
GTATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGA	19830	0.19178324373454878	No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCG	19303	0.18668643236550655	No Hit
ATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGG	19062	0.18435563247947395	No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT	18789	0.1817153487911466	No Hit
GCCTAAATACTCCTACATGACCGATAGTGAACAAGTACCGTGA	18230	0.17630905361980959	No Hit
TATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGAC	17829	0.17243083472230308	No Hit
GAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGCGAAGT	17080	0.1651869794748408	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16419	0.15879420468368918	No Hit
GGATAGATCACTTCGCTTCGGGTCTAGGACATGCGACTGAATC	16305	0.157691668638014	No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGT	16246	0.15712105787753303	No Hit
TCTCAGTACCCACAGGAAGAGAAAACAATAGTGATTCCGTTAG	16030	0.15503204221204323	No Hit
AACAAAGGGTGCTCCAACGGTTTGTAAGCAAACGGTTTCAGGT	15660	0.15145363574800977	No Hit
GGTAGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAG	15481	0.14972246072892334	No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCC	15410	0.14903579354258178	No Hit
AGGCTAAACCGATCATGTGTGATAGCCGGCAGGCGTTGCATGG	15222	0.14721757620409995	No Hit
TCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACA	14858	0.14369719795299676	No Hit
GGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTC	14233	0.13765259243942676	No Hit
GATCTATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGG	13974	0.13514770791460337	No Hit
GGTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGG	13242	0.12806826593711018	No Hit
GGTTTACACCGCCGCTTCGCTGGCCACACGACGCTCTCCTACC	13052	0.1262307058609849	No Hit
CCCTTTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTG	12998	0.12570845194461247	No Hit
GCATTTAGGTGCAGCGTTGCGTGTTTCTTGCCGGAGGTAGAGC	12664	0.12247821475816065	No Hit
CCTAAATGCATTTCGGAGAGAACCAGCTATCACGAAGTTTGAT	12627	0.1221203741117573	No Hit
GCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCT	12589	0.12175286209653226	No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC	12390	0.11982825970101157	No Hit
GTATTTAGGCTTATCAGGTGGTCCTGACAGATTCACACGGGAT	12248	0.11845492532832845	No Hit
ATCCAGTAGCTCTACCTCCGGCAAGAAACACGCAACGCTGCAC	12031	0.11635623829401695	No Hit
CCACAAGGGTGCGGCTTACCAAAAATATCAATGCCACAACTTC	12017	0.11622083913051298	No Hit
GGATAGCCGATGGGGCCCAAAAGCTTACTGACGTTAGCCAAAC	11938	0.11545680099359776	No Hit
GTGTGACAGCGTGCCTTTTGAAGAATGAGCCTGCGAGTTAGTG	11716	0.1133097571151777	No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGTCAGGA	11613	0.11231360612654136	No Hit
CACCACAAGGGTGCGGCTTACCAAAAATATCAATGCCACAACT	11412	0.11036966099337725	No Hit
ATTTCACTCCCCTCCCGGGGTACTTTTCACCTTTCCCTCACGG	11357	0.10983773570818309	No Hit
GAGTATCCCAAGTAGCACGGGGCCCGAGAAATCCCGTGTGAAT	11216	0.10847407270432169	No Hit
GGATTGAAAGCCTGGTCATAGAGGGTGCGAACCCCGTAGTCGA	10944	0.10584346038481603	No Hit
CCATCTAAGGTCCCAAAGCGCGTGCTAAGTGGGAAAGGATGTG	10855	0.10498270855968367	No Hit
ATGTAACGGGGCTCAAGCACGCCACCGAAGTTGTGGCATTGAT	10594	0.10245848129721684	No Hit
ATATAAACCCGTTGTCCATCGACTACGCCTGTCGGCCTCGCCT	10540	0.10193622738084437	No Hit
GGTATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCG	10348	0.10007932456707568	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.671368821711991E-6
2	0.0	0.0	0.0	0.0	9.671368821711991E-6
3	9.671368821711991E-6	0.0	0.0	0.0	9.671368821711991E-6
4	9.671368821711991E-6	0.0	0.0	0.0	9.671368821711991E-6
5	2.9014106465135973E-5	0.0	0.0	9.671368821711991E-6	1.9342737643423982E-5
6	3.8685475286847964E-5	0.0	0.0	1.9342737643423982E-5	1.9342737643423982E-5
7	4.8356844108559955E-5	9.671368821711991E-6	0.0	1.9342737643423982E-5	1.9342737643423982E-5
8	5.8028212930271946E-5	9.671368821711991E-6	0.0	3.8685475286847964E-5	2.9014106465135973E-5
9	6.769958175198394E-5	9.671368821711991E-6	0.0	3.8685475286847964E-5	2.9014106465135973E-5
10	7.737095057369593E-5	9.671368821711991E-6	0.0	5.8028212930271946E-5	2.9014106465135973E-5
11	7.737095057369593E-5	9.671368821711991E-6	0.0	5.8028212930271946E-5	2.9014106465135973E-5
12	8.704231939540792E-5	9.671368821711991E-6	0.0	5.8028212930271946E-5	2.9014106465135973E-5
13	9.671368821711991E-5	1.9342737643423982E-5	0.0	6.769958175198394E-5	2.9014106465135973E-5
14	1.063850570388319E-4	1.9342737643423982E-5	0.0	7.737095057369593E-5	3.8685475286847964E-5
15	1.1605642586054389E-4	1.9342737643423982E-5	0.0	7.737095057369593E-5	7.737095057369593E-5
16	1.1605642586054389E-4	1.9342737643423982E-5	0.0	8.704231939540792E-5	9.671368821711991E-5
17	1.2572779468225588E-4	1.9342737643423982E-5	0.0	9.671368821711991E-5	9.671368821711991E-5
18	1.2572779468225588E-4	1.9342737643423982E-5	0.0	1.1605642586054389E-4	9.671368821711991E-5
19	1.2572779468225588E-4	1.9342737643423982E-5	0.0	1.1605642586054389E-4	1.063850570388319E-4
20	1.6441326996910385E-4	1.9342737643423982E-5	0.0	1.2572779468225588E-4	1.063850570388319E-4
21	1.8375600761252783E-4	1.9342737643423982E-5	0.0	1.4507053232567987E-4	1.063850570388319E-4
22	1.8375600761252783E-4	1.9342737643423982E-5	0.0	1.5474190114739186E-4	1.063850570388319E-4
23	1.8375600761252783E-4	1.9342737643423982E-5	0.0	1.8375600761252783E-4	1.4507053232567987E-4
24	2.030987452559518E-4	1.9342737643423982E-5	0.0	2.224414828993758E-4	1.4507053232567987E-4
25	2.127701140776638E-4	1.9342737643423982E-5	0.0	2.5145558936451177E-4	1.4507053232567987E-4
26	2.5145558936451177E-4	1.9342737643423982E-5	0.0	2.9014106465135973E-4	1.5474190114739186E-4
27	2.6112695818622376E-4	1.9342737643423982E-5	0.0	4.352115969770396E-4	1.5474190114739186E-4
28	2.9014106465135973E-4	1.9342737643423982E-5	0.0	0.001073521939210031	1.5474190114739186E-4
29	2.9014106465135973E-4	1.9342737643423982E-5	0.0	0.0025629127377536777	1.5474190114739186E-4
30	2.9014106465135973E-4	1.9342737643423982E-5	0.0	0.004632585665600044	1.5474190114739186E-4
31	2.9014106465135973E-4	1.9342737643423982E-5	0.0	0.008559161407215111	1.5474190114739186E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8380	0.0	29.781025	1
GTATCAA	21675	0.0	22.46459	1
TCGAAAT	11010	0.0	18.752043	26
CAATGGC	11275	0.0	18.475388	37
CTACGCA	5985	0.0	17.804512	18
TACGCAT	5965	0.0	17.771166	19
GTCGAAA	11800	0.0	17.496609	25
AGTCGAA	11995	0.0	17.335556	24
CGCTACG	6150	0.0	17.206503	16
GCTACGC	6235	0.0	17.031275	17
TCGTCTA	5700	0.0	16.877192	27
GCAATGG	12485	0.0	16.670004	36
ACGCATT	6445	0.0	16.562452	20
CGCATTC	6465	0.0	16.425367	21
AGCAATG	12780	0.0	16.328638	35
CCCGTAG	12780	0.0	16.241783	19
CGGGTTA	6605	0.0	16.21726	34
GCAGCAA	12790	0.0	16.156763	33
ATGCAGC	12825	0.0	16.155945	31
CCGTAGT	12810	0.0	16.131536	20
>>END_MODULE