FastQCFastQC Report
Thu 26 May 2016
SRR1041747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18475660
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT716030.38755313747925646No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC586060.3172065301050138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT473230.2561369932116092No Hit
CTCTATGACCAGGCTTTCAATCCTGTTCGTCTATACAACGTTG406480.22000837859107603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT309420.16747439604322661No Hit
GTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTGCGA303320.1641727548569307No Hit
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTA296990.1607466255603318No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC290060.15699574467163824No Hit
GCCTAAATACTCCTACATGACCGATAGTGAACAAGTACCGTGA267620.14485003512729722No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA250520.13559461475259882No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT248790.13465824766205917No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC237700.12865575573484248No Hit
GTATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGA235760.1276057255870697No Hit
GAATATATAGGGCGGGTAGAGGGAACGTGGGGAAGTGAAACAT231540.1253216393893371No Hit
CTCCTACCCATCAACACGGCTGAACCAACACCACAAGGGTGCG224470.12149498312915478No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCG218250.11812839162443993No Hit
ATCCACAGCTCATCCCCTCCATTTTTAACTGAAGTGGGTTCGG213320.11546001604272865No Hit
CCCTTTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTG196330.10626413345991428No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA189310.10246453983240654No Hit
CCACAAGGGTGCGGCTTACCAAAAATATCAATGCCACAACTTC188860.10222097613833553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137850.027.3641641
GTATCAA371350.023.2700961
TCGAAAT117950.019.4489226
GTCGAAA124450.018.50743325
TCGTCTA84350.018.16004627
AGTCGAA132650.017.62834524
GTTCGTC87150.017.61904725
TGTTCGT89550.017.14684724
GTGCGAA134550.017.10442211
CCGTAGT135600.016.9856220
TTCGTCT90650.016.97959126
CCCGTAG136850.016.84398819
TGCGAAC136450.016.70355612
CCCCGTA138900.016.58207518
ACCCCGT139000.016.55683317
GTAGTCG142050.016.34459722
CGTAGTC144250.016.12097221
TAGTCGA146350.016.07926223
TATCAAC534250.016.060462
CTCTATG104400.016.0191571
Produced by FastQC (version 0.11.5)