FastQCFastQC Report
Thu 26 May 2016
SRR1041746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11771379
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT570440.48459912810555156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT409960.34826845690721536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT263220.22361016496028208No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC181680.15434045577837566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114250.024.790811
GTATCAA277750.021.5272731
TCGAAAT40350.017.74349626
GTCGAAA40750.017.43312825
GTGCGAA43500.016.41609211
AGTCGAA45000.016.19777724
CCGTAGT44050.016.12712720
TGCGAAC44700.016.05816512
TCGTCTA27050.015.93530527
CCCGTAG45300.015.80463619
TAGTCGA47150.015.49840923
CCCCGTA45550.015.47420418
CGTAGTC47350.015.35480621
GTTCGTC28050.015.10338625
GTAGTCG48300.015.01449422
TATCAAC402800.014.7843852
ACCCCGT48650.014.64028817
CGTCTAT29550.014.52453428
TTCGTCT29900.014.35451426
ATCAACG414300.014.2534393
Produced by FastQC (version 0.11.5)