##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041746_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11771379
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01099650261877	31.0	31.0	34.0	30.0	34.0
2	32.29872319972027	33.0	31.0	34.0	31.0	34.0
3	32.372889276608966	34.0	31.0	34.0	31.0	34.0
4	36.00530634516143	37.0	35.0	37.0	35.0	37.0
5	35.863110515768795	37.0	35.0	37.0	35.0	37.0
6	35.98912404400538	37.0	35.0	37.0	35.0	37.0
7	35.94945282111807	37.0	35.0	37.0	35.0	37.0
8	35.93445627738262	37.0	35.0	37.0	35.0	37.0
9	37.5997807053872	39.0	37.0	39.0	35.0	39.0
10	37.48979826407764	39.0	37.0	39.0	35.0	39.0
11	37.56940567456031	39.0	37.0	39.0	35.0	39.0
12	37.4412623193935	39.0	37.0	39.0	35.0	39.0
13	37.46326738778863	39.0	37.0	39.0	35.0	39.0
14	38.32371882682564	40.0	38.0	40.0	35.0	40.0
15	38.32019714937392	40.0	38.0	40.0	35.0	40.0
16	38.30615818248652	40.0	38.0	40.0	35.0	40.0
17	38.29162793925843	40.0	38.0	40.0	34.0	40.0
18	38.255055673596104	40.0	38.0	40.0	34.0	40.0
19	38.28280679774222	40.0	38.0	40.0	34.0	40.0
20	38.23994537938163	40.0	38.0	40.0	34.0	40.0
21	38.20055092950452	40.0	38.0	40.0	34.0	40.0
22	38.138557767955646	40.0	38.0	40.0	34.0	40.0
23	38.08254997141796	40.0	38.0	40.0	34.0	40.0
24	38.04126823203976	40.0	38.0	40.0	34.0	40.0
25	37.9916720887162	40.0	38.0	40.0	34.0	40.0
26	37.854365830885236	40.0	38.0	40.0	34.0	40.0
27	37.77354403422063	40.0	38.0	40.0	33.0	40.0
28	37.70256951203423	40.0	37.0	40.0	33.0	40.0
29	37.61176766120605	40.0	37.0	40.0	33.0	40.0
30	37.521733859728755	40.0	37.0	40.0	33.0	40.0
31	37.420628203373624	40.0	37.0	40.0	33.0	40.0
32	37.29347028924988	39.0	37.0	40.0	32.0	40.0
33	37.227930814223214	39.0	37.0	40.0	32.0	40.0
34	37.12283403669188	39.0	36.0	40.0	32.0	40.0
35	37.035747213644214	39.0	36.0	40.0	31.0	40.0
36	37.155631298592965	39.0	37.0	40.0	32.0	40.0
37	37.16612055393	39.0	37.0	40.0	32.0	40.0
38	37.13896884978387	39.0	37.0	40.0	32.0	40.0
39	37.07898981079447	39.0	36.0	40.0	32.0	40.0
40	37.03852726175922	39.0	36.0	40.0	31.0	40.0
41	36.95616622317572	39.0	36.0	40.0	31.0	40.0
42	36.865737650618506	39.0	36.0	40.0	31.0	40.0
43	36.73801115400328	39.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	4.0
15	11.0
16	18.0
17	49.0
18	139.0
19	329.0
20	810.0
21	1930.0
22	4100.0
23	8040.0
24	14521.0
25	24710.0
26	39041.0
27	59587.0
28	87282.0
29	122226.0
30	164564.0
31	217630.0
32	280814.0
33	367152.0
34	492938.0
35	689217.0
36	1055007.0
37	1884788.0
38	5243854.0
39	1012613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61407087478876	20.037915693649825	14.041421994823205	24.306591436738213
2	16.681427044359033	21.66209243623878	38.198583190635524	23.457897328766663
3	18.838022291186103	25.903948891629437	30.129698483074925	25.12833033410954
4	14.181354622937551	17.900884849600036	37.22372714360824	30.694033383854176
5	14.345889296402742	37.06513060194562	34.11127107537698	14.477709026274663
6	31.021802968029487	37.86040700923826	16.963305658580868	14.154484364151386
7	27.416600892724635	31.798525899132123	22.063481262475705	18.721391945667538
8	25.95239691118602	34.96085717739612	19.654298786913582	19.432447124504275
9	27.111674851349193	15.572763395010899	19.62642609672155	37.68913565691836
10	16.758886108416014	27.408904258371088	31.418315560139554	24.41389407307334
11	34.63017374599867	23.5387289798417	22.641000684796573	19.190096589363066
12	23.615296049851082	24.612485928793898	29.548347733940094	22.223870287414922
13	29.063221904587387	21.56782140817996	25.607084777407984	23.76187190982467
14	23.000219430535708	20.17777186513152	26.938084314505552	29.883924389827225
15	24.48029241094013	27.445985725206878	23.950490422574962	24.123231441278033
16	24.09966580805868	27.35123896698934	24.330879160377048	24.218216064574932
17	23.61005452292378	26.393441244224658	26.1457812207049	23.850723012146666
18	24.441724287358348	25.971757429609564	26.639045433844245	22.947472849187847
19	25.889320189248856	25.50247511357845	26.42481394915583	22.183390748016862
20	24.813167599140254	25.836811472980354	26.21020018130416	23.139820746575232
21	24.706298217056812	25.593551953428733	26.489734125458032	23.210415704056423
22	24.06587197642689	25.575185371229658	26.515669914289564	23.843272738053887
23	23.85822425732788	25.25186726211092	27.134068149534563	23.755840331026636
24	23.461762636306247	25.765341511814377	27.006997226068414	23.765898625810962
25	23.94944551526206	25.736797702291298	26.970765277373193	23.34299150507345
26	23.773748173429805	25.81112204440958	26.98400077000324	23.43112901215737
27	24.158503434474415	26.123557826147643	26.319550156358062	23.398388583019884
28	24.13957617030256	25.714888629446047	26.506078854482556	23.639456345768835
29	24.247617887419988	25.68073800019522	26.42560400102656	23.646040111358236
30	23.849499706024247	26.33105263198135	26.49039674960767	23.32905091238673
31	23.950108139411704	25.641643175366287	26.750026483728035	23.65822220149398
32	24.04832093164276	25.794412022584613	26.50272325782731	23.65454378794532
33	24.449743738605306	25.815777403819894	26.509774258394025	23.224704599180775
34	24.344836743426576	25.60429835790692	26.22929734910413	23.821567549562374
35	24.290841370412082	26.420421940369092	25.907652790722313	23.381083898496513
36	24.208658985493543	25.776852482619073	26.564151914571777	23.45033661731561
37	24.660645112182696	25.436493039600546	26.11100194803005	23.791859900186715
38	24.146176926254775	25.806356247640995	26.273446806869444	23.77402001923479
39	23.931818013845277	25.69231693245116	26.602218822450624	23.77364623125294
40	24.29308409830318	25.647929609606486	26.69332964302653	23.3656566490638
41	24.571624106232584	25.438344989146984	26.381114736005017	23.60891616861542
42	24.573561007593078	25.536948559722696	26.29383524224307	23.595655190441153
43	24.334336699209157	25.12670775446105	26.47620979666019	24.062745749669602
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	488.0
1	872.5
2	1257.0
3	3485.5
4	5714.0
5	5714.0
6	6930.0
7	8146.0
8	7014.5
9	5883.0
10	8323.5
11	10764.0
12	10764.0
13	17534.0
14	24304.0
15	48054.5
16	71805.0
17	81937.5
18	92070.0
19	92070.0
20	85843.0
21	79616.0
22	84728.0
23	89840.0
24	109729.5
25	129619.0
26	129619.0
27	152937.5
28	176256.0
29	206897.5
30	237539.0
31	277158.5
32	316778.0
33	316778.0
34	361556.5
35	406335.0
36	455951.5
37	505568.0
38	563677.0
39	621786.0
40	621786.0
41	674671.5
42	727557.0
43	790067.5
44	852578.0
45	912898.5
46	973219.0
47	973219.0
48	1027113.5
49	1081008.0
50	1087623.5
51	1094239.0
52	1070819.5
53	1047400.0
54	1047400.0
55	989233.0
56	931066.0
57	821281.5
58	711497.0
59	612247.0
60	512997.0
61	512997.0
62	441082.5
63	369168.0
64	316915.0
65	264662.0
66	213930.5
67	163199.0
68	163199.0
69	131220.0
70	99241.0
71	84547.0
72	69853.0
73	54648.0
74	39443.0
75	39443.0
76	30761.5
77	22080.0
78	17379.5
79	12679.0
80	10079.5
81	7480.0
82	7480.0
83	6000.0
84	4520.0
85	3492.0
86	2464.0
87	1679.5
88	895.0
89	895.0
90	583.5
91	272.0
92	167.0
93	62.0
94	41.5
95	21.0
96	21.0
97	14.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.1771379E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.252870675953986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.81658101399981	27.40501225579799
2	14.927963710093975	11.719308579353061
3	5.527793321426714	6.5094526910809485
4	2.7936045908011424	4.386279988898744
5	1.6454315800589254	3.2293956509091815
6	1.0450080837750848	2.4611740300649934
7	0.7497539727712503	2.060099701238083
8	0.5487259781013405	1.7231255883958623
9	0.4168122900696465	1.4724971026426847
>10	2.263161239725914	16.20879568496375
>50	0.16179626975135733	4.370329309517627
>100	0.08503806539949385	6.297514870126424
>500	0.009419877779816477	2.633641745029344
>1k	0.00821796820669351	6.67708711415445
>5k	5.839070952503519E-4	1.5477726276139192
>10k+	1.0813094356487997E-4	1.2985130602130805
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	57044	0.48459912810555156	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40996	0.34826845690721536	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26322	0.22361016496028208	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	18168	0.15434045577837566	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.495181405678977E-6
2	8.495181405678977E-6	0.0	0.0	0.0	8.495181405678977E-6
3	8.495181405678977E-6	0.0	0.0	0.0	8.495181405678977E-6
4	8.495181405678977E-6	0.0	0.0	0.0	8.495181405678977E-6
5	5.946626983975285E-5	8.495181405678977E-6	0.0	8.495181405678977E-6	8.495181405678977E-6
6	8.495181405678977E-5	8.495181405678977E-6	0.0	8.495181405678977E-6	8.495181405678977E-6
7	9.344699546246876E-5	8.495181405678977E-6	0.0	8.495181405678977E-6	8.495181405678977E-6
8	1.189325396795057E-4	8.495181405678977E-6	0.0	8.495181405678977E-6	8.495181405678977E-6
9	1.3592290249086363E-4	8.495181405678977E-6	0.0	1.6990362811357954E-5	8.495181405678977E-6
10	2.0388435373629548E-4	8.495181405678977E-6	0.0	3.398072562271591E-5	8.495181405678977E-6
11	2.633506235760483E-4	8.495181405678977E-6	0.0	3.398072562271591E-5	8.495181405678977E-6
12	2.633506235760483E-4	1.6990362811357954E-5	0.0	5.097108843407387E-5	8.495181405678977E-6
13	2.803409863874063E-4	1.6990362811357954E-5	0.0	5.097108843407387E-5	1.6990362811357954E-5
14	3.6529280044419603E-4	1.6990362811357954E-5	0.0	5.097108843407387E-5	1.6990362811357954E-5
15	3.90778344661233E-4	2.5485544217036935E-5	0.0	6.796145124543182E-5	3.398072562271591E-5
16	3.90778344661233E-4	2.5485544217036935E-5	0.0	6.796145124543182E-5	4.2475907028394886E-5
17	3.90778344661233E-4	2.5485544217036935E-5	0.0	8.495181405678977E-5	4.2475907028394886E-5
18	3.90778344661233E-4	4.2475907028394886E-5	0.0	8.495181405678977E-5	4.2475907028394886E-5
19	3.9927352606691194E-4	4.2475907028394886E-5	0.0	8.495181405678977E-5	4.2475907028394886E-5
20	5.097108843407387E-4	5.097108843407387E-5	0.0	8.495181405678977E-5	4.2475907028394886E-5
21	6.626241496429602E-4	5.097108843407387E-5	0.0	1.4441808389654263E-4	5.946626983975285E-5
22	6.796145124543182E-4	5.097108843407387E-5	0.0	1.6990362811357954E-4	5.946626983975285E-5
23	6.796145124543182E-4	5.097108843407387E-5	0.0	2.2087471654765343E-4	7.64566326511108E-5
24	7.051000566713552E-4	5.097108843407387E-5	0.0	2.803409863874063E-4	7.64566326511108E-5
25	7.220904194827131E-4	5.097108843407387E-5	8.495181405678977E-6	2.8883616779308525E-4	7.64566326511108E-5
26	7.900518707281449E-4	5.097108843407387E-5	8.495181405678977E-6	3.6529280044419603E-4	7.64566326511108E-5
27	7.985470521338239E-4	5.097108843407387E-5	8.495181405678977E-6	6.371386054259234E-4	7.64566326511108E-5
28	8.155374149451819E-4	5.097108843407387E-5	8.495181405678977E-6	0.0018859302720607332	7.64566326511108E-5
29	8.240325963508609E-4	5.097108843407387E-5	8.495181405678977E-6	0.00465535941031208	8.495181405678977E-5
30	8.410229591622188E-4	5.097108843407387E-5	8.495181405678977E-6	0.0088095031176891	8.495181405678977E-5
31	8.580133219735767E-4	5.097108843407387E-5	8.495181405678977E-6	0.016421185657177463	8.495181405678977E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11425	0.0	24.79081	1
GTATCAA	27775	0.0	21.527273	1
TCGAAAT	4035	0.0	17.743496	26
GTCGAAA	4075	0.0	17.433128	25
GTGCGAA	4350	0.0	16.416092	11
AGTCGAA	4500	0.0	16.197777	24
CCGTAGT	4405	0.0	16.127127	20
TGCGAAC	4470	0.0	16.058165	12
TCGTCTA	2705	0.0	15.935305	27
CCCGTAG	4530	0.0	15.804636	19
TAGTCGA	4715	0.0	15.498409	23
CCCCGTA	4555	0.0	15.474204	18
CGTAGTC	4735	0.0	15.354806	21
GTTCGTC	2805	0.0	15.103386	25
GTAGTCG	4830	0.0	15.014494	22
TATCAAC	40280	0.0	14.784385	2
ACCCCGT	4865	0.0	14.640288	17
CGTCTAT	2955	0.0	14.524534	28
TTCGTCT	2990	0.0	14.354514	26
ATCAACG	41430	0.0	14.253439	3
>>END_MODULE