FastQCFastQC Report
Thu 26 May 2016
SRR1041745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16712388
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT895420.535782199408008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT604150.3614983089191084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT393040.23517883859565733No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC282720.16916792501466577No Hit
CTCTATGACCAGGCTTTCAATCCTGTTCGTCTATACAACGTTG185370.11091772163259973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180000.023.0633321
GTATCAA431400.021.30031
TCGAAAT64950.017.88760626
GTCGAAA67550.017.14433725
TCGTCTA41800.017.08373327
AGTCGAA70250.016.7224224
GTGCGAA71950.016.3273111
TGCGAAC72450.016.11249212
CCGTAGT71750.016.089220
GTTCGTC43750.015.98399925
CCCGTAG73300.015.87517119
TAGTCGA74650.015.81111823
TTCGTCT44750.015.75083726
CCCCGTA74550.015.63380218
CGTCTAT46000.015.52391228
TGTTCGT45550.015.4335924
CGTAGTC76550.015.1770121
TATCAAC607950.015.065962
GTAGTCG77000.015.0402622
ACCCCGT78300.014.81417617
Produced by FastQC (version 0.11.5)