##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041745_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16712388
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37688635519951	33.0	31.0	34.0	31.0	34.0
2	32.63781477548272	34.0	31.0	34.0	31.0	34.0
3	32.7005420769312	34.0	31.0	34.0	31.0	34.0
4	36.230204145571534	37.0	35.0	37.0	35.0	37.0
5	36.12350048359337	37.0	35.0	37.0	35.0	37.0
6	36.22644938592857	37.0	37.0	37.0	35.0	37.0
7	36.18995861034342	37.0	36.0	37.0	35.0	37.0
8	36.17477484366687	37.0	36.0	37.0	35.0	37.0
9	37.899882290909	39.0	38.0	39.0	35.0	39.0
10	37.82928968618967	39.0	38.0	39.0	35.0	39.0
11	37.89063795072254	39.0	38.0	39.0	35.0	39.0
12	37.78703821380882	39.0	38.0	39.0	35.0	39.0
13	37.80064081805664	39.0	38.0	39.0	35.0	39.0
14	38.68169695437899	40.0	39.0	40.0	36.0	40.0
15	38.680384095917354	40.0	38.0	40.0	36.0	40.0
16	38.67472033320433	40.0	38.0	40.0	36.0	40.0
17	38.67148506844145	40.0	38.0	40.0	36.0	40.0
18	38.643769519951306	40.0	38.0	40.0	36.0	40.0
19	38.665439732490654	40.0	39.0	40.0	36.0	40.0
20	38.632143533288	40.0	39.0	40.0	36.0	40.0
21	38.59588581835223	40.0	39.0	40.0	36.0	40.0
22	38.544380850899344	40.0	38.0	40.0	35.0	40.0
23	38.494282923541505	40.0	38.0	40.0	35.0	40.0
24	38.46260127517384	40.0	38.0	40.0	35.0	40.0
25	38.42516718735826	40.0	38.0	40.0	35.0	40.0
26	38.31319587601724	40.0	38.0	40.0	35.0	40.0
27	38.25068242790917	40.0	38.0	40.0	35.0	40.0
28	38.18830444817341	40.0	38.0	40.0	34.0	40.0
29	38.10951528889827	40.0	38.0	40.0	34.0	40.0
30	38.03675722464079	40.0	38.0	40.0	34.0	40.0
31	37.94692176845104	40.0	38.0	40.0	34.0	40.0
32	37.83131656589112	40.0	38.0	40.0	34.0	40.0
33	37.77773828611447	40.0	38.0	40.0	33.0	40.0
34	37.67856849661461	40.0	37.0	40.0	33.0	40.0
35	37.60907352079188	40.0	37.0	40.0	33.0	40.0
36	37.71016057070958	40.0	38.0	40.0	33.0	40.0
37	37.72188229473849	40.0	38.0	40.0	33.0	40.0
38	37.69818161234648	40.0	38.0	40.0	33.0	40.0
39	37.6438103280034	40.0	38.0	40.0	33.0	40.0
40	37.608156596172854	40.0	37.0	40.0	33.0	40.0
41	37.535145246747504	40.0	37.0	40.0	33.0	40.0
42	37.452857126103105	40.0	37.0	40.0	33.0	40.0
43	37.339383755331674	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	0.0
13	7.0
14	8.0
15	11.0
16	23.0
17	42.0
18	140.0
19	278.0
20	685.0
21	1679.0
22	3697.0
23	7506.0
24	13608.0
25	23715.0
26	38466.0
27	59505.0
28	88714.0
29	125726.0
30	173393.0
31	231317.0
32	302772.0
33	402116.0
34	548447.0
35	778991.0
36	1213753.0
37	2238883.0
38	7729046.0
39	2729857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.362874772893015	19.318430136973845	13.617718784413096	24.700976305720044
2	16.134881502272446	20.98214809278004	39.157707444322135	23.725262960625376
3	18.325645622875676	25.65806274962022	29.926130245420346	26.09016138208376
4	13.542792328660633	17.338168548982946	37.64797107391236	31.47106804844406
5	14.20774816860403	36.90369084298426	34.191008490228924	14.697552498182784
6	30.7791800908404	37.746700232187045	17.168414232603983	14.305705444368572
7	27.309819518311805	31.800320815912126	22.081996899545413	18.807862766230656
8	25.727675781581905	35.01424811343538	19.69894428013519	19.55913182484753
9	27.0741919108149	15.802002682082298	19.697771497406595	37.42603390969621
10	16.803481345693985	27.275539557841768	31.259614125761082	24.66136497070317
11	34.43138706449372	23.696547734530814	22.693543256654884	19.178521944320583
12	23.731234578804656	24.463655343569094	29.55285624053247	22.25225383709378
13	28.962587512927534	21.644907956899996	25.68619158435048	23.706312945821985
14	23.06102515092397	20.194929653380473	26.99819439328479	29.74585080241076
15	24.48733239079897	27.375812481136748	24.179829956078088	23.957025171986192
16	23.991287181700187	27.399166414757726	24.345557319516516	24.263989084025575
17	23.489066912520222	26.311960923836857	26.252956788700693	23.946015374942228
18	24.4298600535124	25.845474626366983	26.79597912638218	22.92868619373844
19	25.89597010313547	25.433241497265378	26.536261604266247	22.1345267953329
20	24.837916640039712	25.7751794656754	26.255326288499287	23.1315776057856
21	24.65183311924065	25.545254215017028	26.6631315644419	23.139781101300425
22	24.042853720246324	25.41873728637703	26.548833117086556	23.98957587629009
23	23.788449621921178	25.137801970610063	27.318184570631075	23.75556383683768
24	23.300440367947417	25.697955313148547	27.239638045741877	23.76196627316216
25	23.88190724150253	25.7296383975767	27.033808693287874	23.354645667632894
26	23.66120867945383	25.796068162132187	27.12802622820868	23.414696930205306
27	24.073250333824227	26.093362600245996	26.47305100862905	23.360336057300728
28	24.062090947146512	25.668510089641288	26.58623650910929	23.683162454102906
29	24.16434443719234	25.70932412531351	26.504440897375048	23.621890540119104
30	23.744398466574616	26.32596251355581	26.646688671900147	23.28295034796942
31	23.832147745732087	25.659546678787017	26.863061101740815	23.645244473740078
32	23.887053124903517	25.767753836256073	26.651409720741288	23.693783318099126
33	24.386132011774738	25.841226280768492	26.62687702080636	23.145764686650406
34	24.2396777767486	25.59317675008503	26.30166915703489	23.86547631613148
35	24.184024449408426	26.47202183194885	25.950737859843848	23.393215858798875
36	24.106483166858023	25.720501462747276	26.65915846376951	23.513856906625193
37	24.544990219231387	25.373926215691018	26.20608736465429	23.874996200423304
38	24.06195930826881	25.8079575462226	26.33617051016288	23.79391263534571
39	23.846777611912792	25.676121210206464	26.71399802350209	23.763103154378655
40	24.28763022974335	25.612599468131066	26.84485903510617	23.25491126701941
41	24.477698818385498	25.444586375088946	26.46869495849426	23.60901984803129
42	24.503697496731167	25.426665536965753	26.473027074287646	23.596609892015433
43	24.216772612028873	25.094624418724603	26.583256683605	24.105346285641524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	768.0
1	1420.0
2	2072.0
3	5842.0
4	9612.0
5	9612.0
6	11084.0
7	12556.0
8	10714.5
9	8873.0
10	12480.0
11	16087.0
12	16087.0
13	25796.0
14	35505.0
15	70459.0
16	105413.0
17	122350.0
18	139287.0
19	139287.0
20	127013.5
21	114740.0
22	121107.0
23	127474.0
24	156467.5
25	185461.0
26	185461.0
27	217033.5
28	248606.0
29	291229.0
30	333852.0
31	389561.0
32	445270.0
33	445270.0
34	508029.5
35	570789.0
36	640241.0
37	709693.0
38	791980.5
39	874268.0
40	874268.0
41	949905.5
42	1025543.0
43	1111969.0
44	1198395.0
45	1289212.0
46	1380029.0
47	1380029.0
48	1459041.0
49	1538053.0
50	1552251.5
51	1566450.0
52	1537278.5
53	1508107.0
54	1508107.0
55	1421917.5
56	1335728.0
57	1178576.0
58	1021424.0
59	875094.5
60	728765.0
61	728765.0
62	626433.0
63	524101.0
64	449380.0
65	374659.0
66	299166.0
67	223673.0
68	223673.0
69	180011.5
70	136350.0
71	115330.5
72	94311.0
73	73386.5
74	52462.0
75	52462.0
76	40300.0
77	28138.0
78	22194.5
79	16251.0
80	13031.0
81	9811.0
82	9811.0
83	7598.5
84	5386.0
85	4145.5
86	2905.0
87	2015.0
88	1125.0
89	1125.0
90	698.0
91	271.0
92	179.5
93	88.0
94	57.0
95	26.0
96	26.0
97	16.5
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.6712388E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.15799364102535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.5380866528514	21.73111460480215
2	15.980098530721222	10.597360109292255
3	6.4642741279812235	6.430270812883382
4	3.3358277952419955	4.424374272887588
5	1.9877126906683842	3.2954282378683835
6	1.306277663799085	2.598812788179811
7	0.9475179023930476	2.1992454807614443
8	0.6361906896922419	1.687584547463591
9	0.5049556004622997	1.5068983130216163
>10	2.9344863483810606	17.879210159483765
>50	0.22234490059340645	5.0802790454263045
>100	0.11896676418731972	7.2952054575392795
>500	0.01058867986236597	2.416571381376326
>1k	0.01118946512497972	7.898007722016658
>5k	0.0011568296897585422	2.560160880500909
>10k+	3.2535835024459E-4	2.3994761864965
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	89542	0.535782199408008	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	60415	0.3614983089191084	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39304	0.23517883859565733	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	28272	0.16916792501466577	No Hit
CTCTATGACCAGGCTTTCAATCCTGTTCGTCTATACAACGTTG	18537	0.11091772163259973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7950756049943313E-5
2	5.983585349981104E-6	0.0	0.0	0.0	1.7950756049943313E-5
3	5.983585349981104E-6	0.0	0.0	0.0	1.7950756049943313E-5
4	5.983585349981104E-6	0.0	0.0	5.983585349981104E-6	1.7950756049943313E-5
5	5.3852268149829935E-5	0.0	0.0	5.983585349981104E-6	1.7950756049943313E-5
6	1.316388776995843E-4	0.0	0.0	5.983585349981104E-6	2.3934341399924415E-5
7	1.555732190995087E-4	0.0	0.0	5.983585349981104E-6	2.3934341399924415E-5
8	1.9745831654937644E-4	0.0	0.0	5.983585349981104E-6	2.991792674990552E-5
9	2.0344190189935753E-4	0.0	0.0	1.1967170699962207E-5	4.1885097449867724E-5
10	3.171300235489985E-4	0.0	0.0	1.7950756049943313E-5	4.1885097449867724E-5
11	3.7098229169882846E-4	0.0	0.0	2.3934341399924415E-5	4.1885097449867724E-5
12	3.7696587704880955E-4	0.0	0.0	2.991792674990552E-5	4.1885097449867724E-5
13	3.8893304774877173E-4	0.0	0.0	4.1885097449867724E-5	4.1885097449867724E-5
14	4.368017305486206E-4	0.0	0.0	4.1885097449867724E-5	6.581943884979215E-5
15	4.4278531589860167E-4	0.0	0.0	4.1885097449867724E-5	7.778660954975434E-5
16	5.20571925448356E-4	0.0	0.0	5.3852268149829935E-5	7.778660954975434E-5
17	5.265555107983372E-4	0.0	0.0	5.983585349981104E-5	7.778660954975434E-5
18	5.265555107983372E-4	0.0	0.0	6.581943884979215E-5	7.778660954975434E-5
19	5.385226814982994E-4	0.0	0.0	8.377019489973545E-5	7.778660954975434E-5
20	6.581943884979214E-4	5.983585349981104E-6	0.0	1.1967170699962208E-4	7.778660954975434E-5
21	9.753244120469199E-4	5.983585349981104E-6	0.0	1.8549114584941423E-4	8.377019489973545E-5
22	9.932751680968633E-4	5.983585349981104E-6	0.0	2.5131058469920635E-4	8.975378024971655E-5
23	9.992587534468442E-4	5.983585349981104E-6	0.0	2.931956821490741E-4	1.0770453629965987E-4
24	0.001035160265546731	5.983585349981104E-6	0.0	3.590151209988662E-4	1.1368812164964098E-4
25	0.0010590946069466555	5.983585349981104E-6	0.0	3.8893304774877173E-4	1.1368812164964098E-4
26	0.0011308976311464286	5.983585349981104E-6	0.0	5.145883400983749E-4	1.1368812164964098E-4
27	0.0011727827285962964	5.983585349981104E-6	0.0	8.436855343473356E-4	1.1967170699962208E-4
28	0.001202700655346202	5.983585349981104E-6	0.0	0.002584908871191837	1.1967170699962208E-4
29	0.001202700655346202	5.983585349981104E-6	0.0	0.006432354251229687	1.2565529234960317E-4
30	0.001208684240696183	1.7950756049943313E-5	0.0	0.011691925773863077	1.2565529234960317E-4
31	0.001262536508846013	1.7950756049943313E-5	0.0	0.021546890845281955	1.436060483995465E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18000	0.0	23.063332	1
GTATCAA	43140	0.0	21.3003	1
TCGAAAT	6495	0.0	17.887606	26
GTCGAAA	6755	0.0	17.144337	25
TCGTCTA	4180	0.0	17.083733	27
AGTCGAA	7025	0.0	16.72242	24
GTGCGAA	7195	0.0	16.32731	11
TGCGAAC	7245	0.0	16.112492	12
CCGTAGT	7175	0.0	16.0892	20
GTTCGTC	4375	0.0	15.983999	25
CCCGTAG	7330	0.0	15.875171	19
TAGTCGA	7465	0.0	15.811118	23
TTCGTCT	4475	0.0	15.750837	26
CCCCGTA	7455	0.0	15.633802	18
CGTCTAT	4600	0.0	15.523912	28
TGTTCGT	4555	0.0	15.43359	24
CGTAGTC	7655	0.0	15.17701	21
TATCAAC	60795	0.0	15.06596	2
GTAGTCG	7700	0.0	15.04026	22
ACCCCGT	7830	0.0	14.814176	17
>>END_MODULE