FastQCFastQC Report
Thu 26 May 2016
SRR1041744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6698654
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT392660.5861774619199618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT340380.5081319321762252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT255020.38070334726946636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87800.131071107718058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64800.028.8348771
GTATCAA148200.020.4224031
GTGCGAA16250.015.824615511
GTCGAAA15500.015.75483825
TCGAAAT16100.015.39751626
TATACCG3050.015.1639345
TGCGAAC16350.015.16208112
CCCGTAG16550.014.97885219
CCGTAGT16950.014.4070820
AGTCGAA17450.014.3123224
CCCCGTA18000.013.87518
TAGTCGA17900.013.84916123
GTAGTCG18150.013.55647422
CGTAGTC18400.013.37228321
TATCAAC226550.013.20437052
ATCAACG229500.012.9862753
ACCCCGT19100.012.88219817
TCAACGC233100.012.7936514
CAACGCA235750.012.6262995
AACGCAG236950.012.5545476
Produced by FastQC (version 0.11.5)