##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041744_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6698654
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76888506258123	34.0	31.0	34.0	31.0	34.0
2	32.98855844771203	34.0	31.0	34.0	31.0	34.0
3	33.044957688514735	34.0	33.0	34.0	31.0	34.0
4	36.43890787611959	37.0	37.0	37.0	35.0	37.0
5	36.402425323057436	37.0	37.0	37.0	35.0	37.0
6	36.46418579016023	37.0	37.0	37.0	35.0	37.0
7	36.42175308651559	37.0	37.0	37.0	35.0	37.0
8	36.40671603578868	37.0	37.0	37.0	35.0	37.0
9	38.14764622863041	39.0	39.0	39.0	37.0	39.0
10	38.12637464183103	39.0	39.0	39.0	37.0	39.0
11	38.19355888511334	39.0	39.0	39.0	37.0	39.0
12	38.092160753488685	39.0	38.0	39.0	37.0	39.0
13	38.1113847647602	39.0	38.0	39.0	37.0	39.0
14	39.008136559971604	40.0	39.0	40.0	37.0	40.0
15	39.031138942241235	40.0	39.0	40.0	37.0	40.0
16	39.019168776294464	40.0	39.0	40.0	37.0	40.0
17	39.01364706999347	40.0	39.0	40.0	37.0	40.0
18	38.97635972838722	40.0	39.0	40.0	37.0	40.0
19	38.9821349184478	40.0	39.0	40.0	37.0	40.0
20	38.931305304020775	40.0	39.0	40.0	37.0	40.0
21	38.882548195503155	40.0	39.0	40.0	36.0	40.0
22	38.824787935009034	40.0	39.0	40.0	36.0	40.0
23	38.76297626358967	40.0	39.0	40.0	36.0	40.0
24	38.709619275753006	40.0	39.0	40.0	36.0	40.0
25	38.675067259780846	40.0	39.0	40.0	36.0	40.0
26	38.55576553140377	40.0	38.0	40.0	36.0	40.0
27	38.486844372018616	40.0	38.0	40.0	35.0	40.0
28	38.4100926842915	40.0	38.0	40.0	35.0	40.0
29	38.340790403564654	40.0	38.0	40.0	35.0	40.0
30	38.25276077850864	40.0	38.0	40.0	35.0	40.0
31	38.15149416584287	40.0	38.0	40.0	35.0	40.0
32	38.22012302770079	40.0	38.0	40.0	35.0	40.0
33	38.31131433270027	40.0	38.0	40.0	35.0	40.0
34	38.3112562613325	40.0	38.0	40.0	35.0	40.0
35	38.34058289919139	40.0	38.0	40.0	35.0	40.0
36	38.3205233469291	40.0	38.0	40.0	35.0	40.0
37	38.28023599965008	40.0	38.0	40.0	35.0	40.0
38	38.2359327709716	40.0	38.0	40.0	35.0	40.0
39	38.180017955846054	40.0	38.0	40.0	35.0	40.0
40	38.140214138541864	40.0	38.0	40.0	35.0	40.0
41	38.08761357132343	40.0	38.0	40.0	35.0	40.0
42	37.99869317029959	40.0	38.0	40.0	34.0	40.0
43	37.661528271201945	40.0	38.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	1.0
14	4.0
15	6.0
16	10.0
17	18.0
18	40.0
19	92.0
20	216.0
21	517.0
22	1088.0
23	2181.0
24	3897.0
25	6650.0
26	10632.0
27	16326.0
28	24399.0
29	34678.0
30	47533.0
31	63550.0
32	83024.0
33	111165.0
34	156933.0
35	231539.0
36	379471.0
37	797047.0
38	3429056.0
39	1298576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95632316581809	18.254935991618616	13.156717752551483	24.632023090011813
2	17.104062995342048	20.415310299651242	37.26144088051122	25.219185824495487
3	18.526333797804753	23.635658745771913	30.366384052676853	27.47162340374648
4	13.66325234890472	16.529693875814456	35.97706345185167	33.82999032342916
5	14.43400420442674	36.72890105982486	34.182792543098955	14.65430219264945
6	33.24263352010718	36.4695653783581	15.837719040272866	14.45008206126186
7	28.86641107303049	31.592197477284245	20.74156987358953	18.799821576095734
8	27.370214971545032	34.61349100879072	18.596482218666615	19.419811800997632
9	26.954728517102094	14.710686654363696	18.84277050285027	39.49181432568394
10	15.886549745665324	28.34332091193246	32.03098712069619	23.739142221706032
11	35.64021966203957	22.359223211110766	22.273668710161772	19.726888416687892
12	23.12304830194245	24.69446847083011	29.4890436198078	22.69343960741964
13	29.57312021191123	21.232056469852004	25.227784566869705	23.967038751367063
14	22.677137825001857	20.34755041833777	26.747657066628612	30.22765469003176
15	24.508013102333692	28.227178176391853	23.34134588829338	23.923462832981073
16	24.056683626292685	27.436123734708495	24.497100462271973	24.010092176726847
17	23.423974428295594	26.89895611864712	26.070730030241897	23.606339422815388
18	24.075568017097165	26.20659314542892	26.758524921573795	22.959313915900122
19	25.321878096704204	25.718987724996694	26.76009240065243	22.199041777646674
20	24.444970586628298	25.786060901189998	26.62625954408154	23.14270896810016
21	24.337575877183685	25.633119728231968	27.03962616967528	22.989678224909063
22	23.999284035270367	25.411030335347967	26.95749026595492	23.632195363426742
23	23.647900010957425	25.350540571284917	27.347314848624816	23.654244569132842
24	23.378413036410002	25.659766872568728	27.315771198213852	23.64604889280742
25	23.655498552395752	25.598888970829066	27.25935687975525	23.48625559701994
26	23.60733962375128	25.809737299463443	27.310143201902946	23.27277987488233
27	23.81712803796106	26.03491686538818	26.768556787677046	23.379398308973716
28	23.8383561831974	25.760817023837923	26.924528420187098	23.476298372777578
29	23.85494160468655	25.733811598568906	26.930663981152037	23.480582815592506
30	23.751950765034287	26.126144147764613	26.829524259649773	23.292380827551327
31	23.803140153230785	25.540115969566422	27.22782815771646	23.428915719486334
32	23.891829612337045	25.698043218831724	26.897388639568486	23.512738529262744
33	23.987983257532036	25.676217938708284	26.94745839985167	23.38834040390801
34	24.00079180086029	25.555686261747507	26.792173472461783	23.651348464930415
35	23.877647658768463	26.01228545316716	26.613092719821026	23.49697416824335
36	23.86804871545836	25.837504071713514	26.953175966395637	23.341271246432495
37	24.17757358418572	25.43923003039118	26.833301137810672	23.549895247612433
38	23.878349292260804	25.686936509931694	26.85472335188532	23.57999084592218
39	23.69140128748253	25.763951981995188	27.083530512249176	23.461116218273105
40	23.964694996935204	25.595843582904866	27.141975089323918	23.297486330836016
41	24.14604486214693	25.60572616528634	26.956967772928706	23.291261199638015
42	24.113008374518223	25.618310783031934	26.82549359916186	23.44318724328798
43	23.938047852598448	25.29345447607833	26.840078618779234	23.92841905254399
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4054.0
1	3402.5
2	2751.0
3	4604.0
4	6457.0
5	6457.0
6	6917.5
7	7378.0
8	5737.5
9	4097.0
10	5832.0
11	7567.0
12	7567.0
13	12568.5
14	17570.0
15	34684.0
16	51798.0
17	54931.0
18	58064.0
19	58064.0
20	55755.0
21	53446.0
22	50453.0
23	47460.0
24	57429.5
25	67399.0
26	67399.0
27	80424.0
28	93449.0
29	109759.0
30	126069.0
31	148008.0
32	169947.0
33	169947.0
34	196789.0
35	223631.0
36	252309.0
37	280987.0
38	313426.0
39	345865.0
40	345865.0
41	377145.5
42	408426.0
43	442286.0
44	476146.0
45	510173.5
46	544201.0
47	544201.0
48	569742.5
49	595284.0
50	602033.5
51	608783.0
52	592774.5
53	576766.0
54	576766.0
55	550103.0
56	523440.0
57	471549.5
58	419659.0
59	369254.0
60	318849.0
61	318849.0
62	275298.0
63	231747.0
64	197697.0
65	163647.0
66	133259.0
67	102871.0
68	102871.0
69	82398.0
70	61925.0
71	50898.5
72	39872.0
73	32698.0
74	25524.0
75	25524.0
76	20027.5
77	14531.0
78	11626.5
79	8722.0
80	6905.0
81	5088.0
82	5088.0
83	3971.0
84	2854.0
85	2166.5
86	1479.0
87	1023.5
88	568.0
89	568.0
90	379.0
91	190.0
92	125.5
93	61.0
94	39.5
95	18.0
96	18.0
97	13.5
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6698654.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.19359454974781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97570598257855	33.495986910239225
2	15.195802305106662	14.342890656906537
3	5.413874935011774	7.665006558779637
4	2.632060551966633	4.968655940795948
5	1.454987459301923	3.4333044114631317
6	0.9351295360176256	2.647927450458577
7	0.648909218374259	2.1437050986083976
8	0.45725734010981867	1.7263694011231134
9	0.351524502444194	1.493073435838781
>10	1.7840202544778818	14.872223456296993
>50	0.09464681339051734	3.049499236437111
>100	0.048121471647628186	4.385330537401691
>500	0.004807682148763587	1.5779976946677852
>1k	0.0029927581603680655	2.482501070880081
>5k	6.367570536537152E-5	0.23092578922919513
>10k+	9.551355804805727E-5	1.4846023508738273
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	39266	0.5861774619199618	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34038	0.5081319321762252	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25502	0.38070334726946636	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8780	0.131071107718058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.4928372177455352E-5	0.0	0.0	0.0	0.0
3	1.4928372177455352E-5	0.0	0.0	2.9856744354910703E-5	0.0
4	1.4928372177455352E-5	0.0	0.0	7.464186088727675E-5	0.0
5	2.9856744354910703E-5	1.4928372177455352E-5	0.0	8.95702330647321E-5	0.0
6	5.9713488709821407E-5	1.4928372177455352E-5	0.0	8.95702330647321E-5	0.0
7	5.9713488709821407E-5	1.4928372177455352E-5	0.0	1.0449860524218746E-4	0.0
8	7.464186088727675E-5	1.4928372177455352E-5	0.0	1.0449860524218746E-4	0.0
9	7.464186088727675E-5	1.4928372177455352E-5	0.0	2.3885395483928563E-4	1.4928372177455352E-5
10	8.95702330647321E-5	1.4928372177455352E-5	0.0	3.8813767661383916E-4	1.4928372177455352E-5
11	1.1942697741964281E-4	1.4928372177455352E-5	0.0	4.030660487912945E-4	1.4928372177455352E-5
12	1.1942697741964281E-4	1.4928372177455352E-5	0.0	4.4785116532366056E-4	1.4928372177455352E-5
13	1.1942697741964281E-4	1.4928372177455352E-5	0.0	4.4785116532366056E-4	1.4928372177455352E-5
14	1.1942697741964281E-4	1.4928372177455352E-5	0.0	4.4785116532366056E-4	2.9856744354910703E-5
15	1.1942697741964281E-4	1.4928372177455352E-5	0.0	4.926362818560265E-4	2.9856744354910703E-5
16	1.1942697741964281E-4	1.4928372177455352E-5	0.0	5.07564654033482E-4	2.9856744354910703E-5
17	1.1942697741964281E-4	1.4928372177455352E-5	0.0	5.52349770565848E-4	4.478511653236605E-5
18	1.1942697741964281E-4	4.478511653236605E-5	0.0	5.822065149207587E-4	4.478511653236605E-5
19	1.3435534959709816E-4	4.478511653236605E-5	0.0	5.822065149207587E-4	4.478511653236605E-5
20	1.492837217745535E-4	4.478511653236605E-5	0.0	6.867051201629461E-4	4.478511653236605E-5
21	1.6421209395200886E-4	4.478511653236605E-5	0.0	8.50917214114955E-4	4.478511653236605E-5
22	1.6421209395200886E-4	4.478511653236605E-5	0.0	0.0012241265185513389	4.478511653236605E-5
23	1.6421209395200886E-4	4.478511653236605E-5	0.0	0.0014629804733906245	7.464186088727675E-5
24	1.6421209395200886E-4	4.478511653236605E-5	0.0	0.001866046522181919	7.464186088727675E-5
25	1.6421209395200886E-4	4.478511653236605E-5	0.0	0.002164613965731026	7.464186088727675E-5
26	1.9406883830691958E-4	4.478511653236605E-5	0.0	0.0026124651310546866	7.464186088727675E-5
27	1.9406883830691958E-4	4.478511653236605E-5	0.0	0.0035977376947667395	8.95702330647321E-5
28	1.9406883830691958E-4	4.478511653236605E-5	0.0	0.007956822370583703	8.95702330647321E-5
29	1.9406883830691958E-4	4.478511653236605E-5	0.0	0.017003415910121646	8.95702330647321E-5
30	1.9406883830691958E-4	4.478511653236605E-5	0.0	0.028513190858939722	8.95702330647321E-5
31	1.9406883830691958E-4	4.478511653236605E-5	0.0	0.046098813283982125	8.95702330647321E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6480	0.0	28.834877	1
GTATCAA	14820	0.0	20.422403	1
GTGCGAA	1625	0.0	15.8246155	11
GTCGAAA	1550	0.0	15.754838	25
TCGAAAT	1610	0.0	15.397516	26
TATACCG	305	0.0	15.163934	5
TGCGAAC	1635	0.0	15.162081	12
CCCGTAG	1655	0.0	14.978852	19
CCGTAGT	1695	0.0	14.40708	20
AGTCGAA	1745	0.0	14.31232	24
CCCCGTA	1800	0.0	13.875	18
TAGTCGA	1790	0.0	13.849161	23
GTAGTCG	1815	0.0	13.556474	22
CGTAGTC	1840	0.0	13.372283	21
TATCAAC	22655	0.0	13.2043705	2
ATCAACG	22950	0.0	12.986275	3
ACCCCGT	1910	0.0	12.882198	17
TCAACGC	23310	0.0	12.793651	4
CAACGCA	23575	0.0	12.626299	5
AACGCAG	23695	0.0	12.554547	6
>>END_MODULE