FastQCFastQC Report
Thu 26 May 2016
SRR1041743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7045831
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT279260.3963478544972197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT252180.3579137790843976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT200720.2848776815680081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60350.028.4473921
GTATCAA130400.019.7626531
GTGCGAA14800.015.7511
CGGGTTA7550.015.6821234
TGCGAAC14850.015.57239112
TCGAAAT14850.015.57239126
GTCGAAA14950.015.22073625
TAGTCGA15300.014.993463523
CCGTAGT14950.014.97324420
CCCGTAG15600.014.58653919
TACGCAT8400.014.31547519
CGTAGTC16150.014.08978421
GTAGTCG16400.013.98780422
AGTCGAA17000.013.38529324
CCCCGTA17050.013.34604218
CGCTACG9050.013.287292516
GTATTAT8800.013.2443181
TCGGGTT9600.013.10416733
TATCAAC196600.013.0233972
ACGCATT9600.012.91145920
Produced by FastQC (version 0.11.5)