##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041743_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7045831
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70256240889116	34.0	31.0	34.0	31.0	34.0
2	32.94004780983251	34.0	31.0	34.0	31.0	34.0
3	33.00143332986556	34.0	31.0	34.0	31.0	34.0
4	36.422277939961944	37.0	37.0	37.0	35.0	37.0
5	36.37555939107821	37.0	37.0	37.0	35.0	37.0
6	36.44316121121838	37.0	37.0	37.0	35.0	37.0
7	36.402044130777476	37.0	37.0	37.0	35.0	37.0
8	36.38390659668107	37.0	37.0	37.0	35.0	37.0
9	38.1132456909625	39.0	38.0	39.0	37.0	39.0
10	38.07713582684569	39.0	38.0	39.0	37.0	39.0
11	38.152323977114975	39.0	38.0	39.0	37.0	39.0
12	38.04184630599286	39.0	38.0	39.0	35.0	39.0
13	38.06496792216561	39.0	38.0	39.0	37.0	39.0
14	38.972899293213246	40.0	39.0	40.0	37.0	40.0
15	38.997167261037056	40.0	39.0	40.0	37.0	40.0
16	38.976689761647705	40.0	39.0	40.0	37.0	40.0
17	38.96315381393621	40.0	39.0	40.0	37.0	40.0
18	38.91726625290899	40.0	39.0	40.0	37.0	40.0
19	38.91921222635059	40.0	39.0	40.0	37.0	40.0
20	38.86112965241431	40.0	39.0	40.0	36.0	40.0
21	38.80744130820055	40.0	39.0	40.0	36.0	40.0
22	38.745138224291786	40.0	39.0	40.0	36.0	40.0
23	38.68034898935271	40.0	39.0	40.0	36.0	40.0
24	38.62461816072511	40.0	39.0	40.0	36.0	40.0
25	38.58423427981739	40.0	38.0	40.0	36.0	40.0
26	38.45993098613918	40.0	38.0	40.0	35.0	40.0
27	38.38289252183312	40.0	38.0	40.0	35.0	40.0
28	38.30457968690989	40.0	38.0	40.0	35.0	40.0
29	38.23329455390003	40.0	38.0	40.0	35.0	40.0
30	38.139156758088575	40.0	38.0	40.0	34.0	40.0
31	38.04338083045137	40.0	38.0	40.0	34.0	40.0
32	38.135156520217414	40.0	38.0	40.0	35.0	40.0
33	38.245568194865875	40.0	38.0	40.0	35.0	40.0
34	38.254525548512305	40.0	38.0	40.0	35.0	40.0
35	38.28555453572474	40.0	38.0	40.0	35.0	40.0
36	38.27261127892508	40.0	38.0	40.0	35.0	40.0
37	38.22638919383675	40.0	38.0	40.0	35.0	40.0
38	38.182192136030515	40.0	38.0	40.0	35.0	40.0
39	38.122137473918976	40.0	38.0	40.0	35.0	40.0
40	38.083125751951755	40.0	38.0	40.0	35.0	40.0
41	38.0265413689315	40.0	38.0	40.0	34.0	40.0
42	37.93398848198318	40.0	38.0	40.0	34.0	40.0
43	37.56454604148183	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	4.0
15	7.0
16	17.0
17	26.0
18	62.0
19	102.0
20	272.0
21	632.0
22	1279.0
23	2388.0
24	4213.0
25	7323.0
26	11728.0
27	17802.0
28	25695.0
29	37154.0
30	50563.0
31	67518.0
32	88982.0
33	119491.0
34	169491.0
35	251254.0
36	421647.0
37	916060.0
38	3779394.0
39	1072725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93341253856358	18.10534484860622	13.179268705139252	24.781973907690947
2	17.47823642094169	19.95127331325432	36.894072537362874	25.676417728441116
3	19.146016985079545	22.802320975339885	29.857386587898578	28.194275451681992
4	14.143640402388305	16.325852834108566	34.89625283376794	34.63425392973519
5	14.886590382312603	36.458140991460056	33.68880122160182	14.966467404625517
6	34.44737746335386	35.3935256181989	15.422183699836115	14.73691321861112
7	29.934212160354118	30.991674367437994	19.933929723832435	19.14018374837546
8	27.80694001885654	34.540709250619265	18.080649393946576	19.571701336577615
9	26.85989771823934	14.895574418404301	18.53010666875206	39.7144211946043
10	16.423896627665354	28.1141145735684	31.389200223508055	24.07278857525819
11	36.41736510569158	21.882358518108084	21.64501249036487	20.055263885835465
12	23.56881679393105	24.30679929734335	28.818389200649293	23.30599470807631
13	29.980636776556235	20.604751944802537	24.774735584773463	24.639875693867765
14	22.85931922011754	19.949201165909315	26.016008615591264	31.175470998381883
15	24.880486063318862	27.817300755581563	22.693334540666672	24.608878640432902
16	24.652251806777652	27.0272449055335	23.905356231223827	24.415147056465024
17	24.00385419406171	26.59900585182926	25.446778385686514	23.95036156842252
18	24.657034777019206	25.948323199917795	25.906198999096063	23.48844302396694
19	25.84562417122977	25.39968670835278	25.891353340720208	22.86333577969724
20	25.10679010041541	25.487554271454993	25.78706471954834	23.618590908581258
21	24.900710789117706	25.25662338480727	26.198414353111794	23.644251472963234
22	24.575241728051665	25.103270288486907	26.114449807269008	24.20703817619242
23	24.14195004109522	25.055639285131875	26.458923014190944	24.343487659581957
24	23.96590267351005	25.326423526195846	26.40889342932012	24.298780370973986
25	24.308942408638526	25.264117177945373	26.360794063893955	24.06614634952215
26	24.174366373533513	25.493373315369045	26.401981540573427	23.930278770524016
27	24.37024106879657	25.692114954219026	25.956881452308465	23.98076252467594
28	24.417744337041295	25.443187609807843	26.058984383815055	24.08008366933581
29	24.401805833832803	25.44267666936661	26.064150559387528	24.09136693741306
30	24.344878553005316	25.786355080046626	26.006967808339425	23.86179855860863
31	24.426345167802065	25.279828596513315	26.289915270462778	24.003910965221845
32	24.47011573226778	25.41096997643003	26.04347166430759	24.0754426269946
33	24.49117783267864	25.369299944889395	26.082885042232775	24.056637180199182
34	24.540526163627824	25.249186362829313	25.97213870159531	24.238148771947554
35	24.422754391923394	25.667462077929486	25.860271130545144	24.049512399601976
36	24.472273036352988	25.502116074030162	26.121461045545942	23.90414984407091
37	24.74690352351625	25.176661205754154	25.968391805026265	24.108043465703336
38	24.393914642573744	25.468266269798413	25.970549109111474	24.167269978516373
39	24.28971117814208	25.513016136776486	26.17190222132776	24.025370463753674
40	24.532961406539556	25.31428869071654	26.24622986273727	23.906520040006637
41	24.640202128038553	25.309377985364677	26.13045927442767	23.919960612169096
42	24.60260542723775	25.324252029320597	26.011608850680634	24.06153369276101
43	24.489943059945663	24.981212294192126	26.085411358858877	24.443433287003337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1120.0
1	1092.0
2	1064.0
3	2232.5
4	3401.0
5	3401.0
6	3860.0
7	4319.0
8	3493.5
9	2668.0
10	3778.5
11	4889.0
12	4889.0
13	8568.0
14	12247.0
15	25339.0
16	38431.0
17	40259.5
18	42088.0
19	42088.0
20	41653.5
21	41219.0
22	38840.5
23	36462.0
24	45290.5
25	54119.0
26	54119.0
27	66336.0
28	78553.0
29	94923.5
30	111294.0
31	133570.5
32	155847.0
33	155847.0
34	183443.5
35	211040.0
36	243176.0
37	275312.0
38	311470.0
39	347628.0
40	347628.0
41	384256.0
42	420884.0
43	461129.0
44	501374.0
45	540361.0
46	579348.0
47	579348.0
48	609188.5
49	639029.0
50	648726.0
51	658423.0
52	646037.5
53	633652.0
54	633652.0
55	607230.5
56	580809.0
57	527126.5
58	473444.0
59	419051.5
60	364659.0
61	364659.0
62	316607.5
63	268556.0
64	229465.5
65	190375.0
66	155911.0
67	121447.0
68	121447.0
69	97545.0
70	73643.0
71	61006.5
72	48370.0
73	39350.0
74	30330.0
75	30330.0
76	23796.0
77	17262.0
78	13817.5
79	10373.0
80	8229.0
81	6085.0
82	6085.0
83	4675.5
84	3266.0
85	2505.0
86	1744.0
87	1195.0
88	646.0
89	646.0
90	470.5
91	295.0
92	185.0
93	75.0
94	52.5
95	30.0
96	30.0
97	17.0
98	4.0
99	5.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7045831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.704888717954276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84998168962522	33.09040510483044
2	14.859224382682854	13.87996842456631
3	5.32556783003005	7.461901585844122
4	2.7169826204882783	5.075854837540834
5	1.578503514202231	3.686191548585748
6	0.9820424689908184	2.7519710538312743
7	0.6684325741342552	2.185334829327727
8	0.48125767934722385	1.7981669086858407
9	0.38593143634962546	1.6222396308722558
>10	1.9865129070818701	16.570930107802244
>50	0.10893439008665345	3.43975664004584
>100	0.050574808551511696	4.398389433922949
>500	0.0038218547973756646	1.2375719608607223
>1k	0.002078977630643368	1.5823472452815357
>5k	6.114640055713186E-5	0.1735063060657689
>10k+	9.171960083569778E-5	1.045464381936576
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27926	0.3963478544972197	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25218	0.3579137790843976	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20072	0.2848776815680081	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4192790034277008E-5
2	0.0	0.0	0.0	1.4192790034277008E-5	1.4192790034277008E-5
3	0.0	0.0	0.0	2.8385580068554015E-5	1.4192790034277008E-5
4	0.0	0.0	0.0	7.096395017138503E-5	1.4192790034277008E-5
5	0.0	0.0	0.0	1.1354232027421606E-4	1.4192790034277008E-5
6	1.4192790034277008E-5	0.0	1.4192790034277008E-5	1.1354232027421606E-4	2.8385580068554015E-5
7	1.4192790034277008E-5	1.4192790034277008E-5	1.4192790034277008E-5	1.1354232027421606E-4	2.8385580068554015E-5
8	1.4192790034277008E-5	1.4192790034277008E-5	1.4192790034277008E-5	1.2773511030849308E-4	2.8385580068554015E-5
9	1.4192790034277008E-5	1.4192790034277008E-5	1.4192790034277008E-5	2.2708464054843212E-4	4.257837010283102E-5
10	2.8385580068554015E-5	1.4192790034277008E-5	1.4192790034277008E-5	3.2643417078837114E-4	4.257837010283102E-5
11	8.515674020566205E-5	1.4192790034277008E-5	1.4192790034277008E-5	3.5481975085692517E-4	4.257837010283102E-5
12	8.515674020566205E-5	1.4192790034277008E-5	1.4192790034277008E-5	3.832053309254792E-4	4.257837010283102E-5
13	8.515674020566205E-5	2.8385580068554015E-5	1.4192790034277008E-5	3.973981209597562E-4	4.257837010283102E-5
14	9.934953023993905E-5	2.8385580068554015E-5	1.4192790034277008E-5	3.973981209597562E-4	8.515674020566205E-5
15	9.934953023993905E-5	2.8385580068554015E-5	1.4192790034277008E-5	4.257837010283102E-4	9.934953023993905E-5
16	1.5612069037704708E-4	2.8385580068554015E-5	1.4192790034277008E-5	4.6836207113114123E-4	1.1354232027421606E-4
17	1.5612069037704708E-4	2.8385580068554015E-5	1.4192790034277008E-5	5.251332312682492E-4	1.1354232027421606E-4
18	1.5612069037704708E-4	2.8385580068554015E-5	1.4192790034277008E-5	5.535188113368033E-4	1.2773511030849308E-4
19	1.703134804113241E-4	2.8385580068554015E-5	1.4192790034277008E-5	6.244827615081883E-4	1.2773511030849308E-4
20	1.845062704456011E-4	2.8385580068554015E-5	1.4192790034277008E-5	7.664106618509584E-4	1.2773511030849308E-4
21	2.128918505141551E-4	2.8385580068554015E-5	1.4192790034277008E-5	9.509169322965595E-4	1.2773511030849308E-4
22	2.2708464054843212E-4	2.8385580068554015E-5	1.4192790034277008E-5	0.0011638087828107146	1.2773511030849308E-4
23	2.2708464054843212E-4	2.8385580068554015E-5	1.4192790034277008E-5	0.0013908934233591466	1.4192790034277006E-4
24	2.412774305827091E-4	2.8385580068554015E-5	1.4192790034277008E-5	0.0017740987542846259	1.4192790034277006E-4
25	2.412774305827091E-4	2.8385580068554015E-5	1.4192790034277008E-5	0.001958605024730227	1.4192790034277006E-4
26	2.838558006855401E-4	2.8385580068554015E-5	1.4192790034277008E-5	0.0024127743058270914	1.4192790034277006E-4
27	2.9804859071981717E-4	2.8385580068554015E-5	1.4192790034277008E-5	0.003363691238123651	1.4192790034277006E-4
28	2.9804859071981717E-4	2.8385580068554015E-5	1.4192790034277008E-5	0.007380250817824043	1.4192790034277006E-4
29	3.1224138075409415E-4	2.8385580068554015E-5	1.4192790034277008E-5	0.014164404454208452	1.4192790034277006E-4
30	3.1224138075409415E-4	2.8385580068554015E-5	1.4192790034277008E-5	0.023730344937311156	1.4192790034277006E-4
31	3.2643417078837114E-4	2.8385580068554015E-5	1.4192790034277008E-5	0.03895920864409039	1.4192790034277006E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6035	0.0	28.447392	1
GTATCAA	13040	0.0	19.762653	1
GTGCGAA	1480	0.0	15.75	11
CGGGTTA	755	0.0	15.68212	34
TGCGAAC	1485	0.0	15.572391	12
TCGAAAT	1485	0.0	15.572391	26
GTCGAAA	1495	0.0	15.220736	25
TAGTCGA	1530	0.0	14.9934635	23
CCGTAGT	1495	0.0	14.973244	20
CCCGTAG	1560	0.0	14.586539	19
TACGCAT	840	0.0	14.315475	19
CGTAGTC	1615	0.0	14.089784	21
GTAGTCG	1640	0.0	13.987804	22
AGTCGAA	1700	0.0	13.385293	24
CCCCGTA	1705	0.0	13.346042	18
CGCTACG	905	0.0	13.2872925	16
GTATTAT	880	0.0	13.244318	1
TCGGGTT	960	0.0	13.104167	33
TATCAAC	19660	0.0	13.023397	2
ACGCATT	960	0.0	12.911459	20
>>END_MODULE