FastQCFastQC Report
Thu 26 May 2016
SRR1041742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5712192
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT265760.46525046777139145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT235270.411873410417577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT197000.344876362699293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52050.028.469741
GTATCAA107000.019.3990651
TGCGAAC6250.016.57612
GTGCGAA6450.016.34883711
CCGTAGT6550.016.09923720
GTCGAAA6400.015.898437525
TAGTCGA7450.014.89932823
TCGAAAT7050.014.69503626
TATACCG2009.858923E-1013.8750015
CGGGTTA3600.013.87534
GTAGTCG7650.013.54248322
CGTAGTC7800.013.5192321
AGTCGAA8250.013.45454624
GTATAGG6750.013.4296291
TATCAAC160700.012.8245172
CCCCGTA8250.012.55757618
CCCGTAG8250.012.55757619
ATCAACG163400.012.5220323
TCAACGC164550.012.4120334
GTATTAT6900.012.3333331
Produced by FastQC (version 0.11.5)