##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041742_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5712192
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75351756383539	34.0	31.0	34.0	31.0	34.0
2	32.97641203236866	34.0	31.0	34.0	31.0	34.0
3	33.0354179271285	34.0	33.0	34.0	31.0	34.0
4	36.430649565000614	37.0	37.0	37.0	35.0	37.0
5	36.38825305591969	37.0	37.0	37.0	35.0	37.0
6	36.45083936254244	37.0	37.0	37.0	35.0	37.0
7	36.4100082770327	37.0	37.0	37.0	35.0	37.0
8	36.392948101184274	37.0	37.0	37.0	35.0	37.0
9	38.12625573510134	39.0	39.0	39.0	37.0	39.0
10	38.10619040816555	39.0	38.0	39.0	37.0	39.0
11	38.17279601245897	39.0	39.0	39.0	37.0	39.0
12	38.070287378295404	39.0	38.0	39.0	37.0	39.0
13	38.09184547718284	39.0	38.0	39.0	37.0	39.0
14	38.99383301541685	40.0	39.0	40.0	37.0	40.0
15	39.01495625497182	40.0	39.0	40.0	37.0	40.0
16	38.997400822661426	40.0	39.0	40.0	37.0	40.0
17	38.9860913288629	40.0	39.0	40.0	37.0	40.0
18	38.9467936301861	40.0	39.0	40.0	37.0	40.0
19	38.95369045718351	40.0	39.0	40.0	37.0	40.0
20	38.900229369040815	40.0	39.0	40.0	37.0	40.0
21	38.85023840235062	40.0	39.0	40.0	36.0	40.0
22	38.78835917980348	40.0	39.0	40.0	36.0	40.0
23	38.72565785603845	40.0	39.0	40.0	36.0	40.0
24	38.673030073218825	40.0	39.0	40.0	36.0	40.0
25	38.63458598730575	40.0	39.0	40.0	36.0	40.0
26	38.51238596321692	40.0	38.0	40.0	35.0	40.0
27	38.437298676234974	40.0	38.0	40.0	35.0	40.0
28	38.359758215410125	40.0	38.0	40.0	35.0	40.0
29	38.2921480930613	40.0	38.0	40.0	35.0	40.0
30	38.19507292471962	40.0	38.0	40.0	35.0	40.0
31	38.10039596007977	40.0	38.0	40.0	34.0	40.0
32	38.178644030172656	40.0	38.0	40.0	35.0	40.0
33	38.280495823669796	40.0	38.0	40.0	35.0	40.0
34	38.28590337999843	40.0	38.0	40.0	35.0	40.0
35	38.31628908832196	40.0	38.0	40.0	35.0	40.0
36	38.30100843949223	40.0	38.0	40.0	35.0	40.0
37	38.25317566356313	40.0	38.0	40.0	35.0	40.0
38	38.2118206110719	40.0	38.0	40.0	35.0	40.0
39	38.158155923330305	40.0	38.0	40.0	35.0	40.0
40	38.116551579498726	40.0	38.0	40.0	35.0	40.0
41	38.05864001069992	40.0	38.0	40.0	35.0	40.0
42	37.97084201651485	40.0	38.0	40.0	34.0	40.0
43	37.61845067532744	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	1.0
12	2.0
13	1.0
14	7.0
15	7.0
16	18.0
17	16.0
18	52.0
19	101.0
20	200.0
21	470.0
22	1022.0
23	1894.0
24	3669.0
25	5982.0
26	9603.0
27	14567.0
28	21312.0
29	29981.0
30	41520.0
31	55536.0
32	73314.0
33	97511.0
34	136414.0
35	200680.0
36	328841.0
37	693699.0
38	2928664.0
39	1067106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.192912283060515	17.936231835344472	13.197140432254379	24.673715449340637
2	17.284765638129812	19.804271284998823	37.19797933963004	25.712983737241323
3	19.254079694800176	22.65114688021691	29.66407641759941	28.430697007383504
4	14.057265582109284	16.08860486482247	34.92557673131436	34.92855282175389
5	14.914449654353353	36.21017990991899	33.871778119502984	15.003592316224665
6	34.65715088008246	35.21830148566435	15.421400401106967	14.703147233146224
7	30.004208542009792	30.933466522133713	19.857473278209138	19.204851657647364
8	27.688337506862513	34.815268814493635	18.116635435223465	19.37975824342039
9	26.618415487434593	15.16634945043864	18.595768489574578	39.619466572552184
10	16.35773097262837	28.26233782057746	31.48125973356638	23.89867147322779
11	36.19188570692302	22.114120113609626	21.617095503792587	20.076898675674766
12	23.483629401812824	24.430918988717465	28.88255506817698	23.20289654129273
13	29.93803429576597	20.61942245638802	24.7911134639732	24.65142978387281
14	22.698029057846796	20.05438542682039	25.996955284416213	31.25063023091661
15	24.654125771682743	27.893127541931364	22.768807491064727	24.683939195321166
16	24.53641264159188	27.142487507422718	24.025225342565516	24.295874508419885
17	23.816741454068772	26.81333190480992	25.510907196396758	23.859019444724545
18	24.420380127278634	26.108366105341	26.04782892451794	23.423424842862424
19	25.494416154078852	25.621635267161892	26.12849848184375	22.75545009691551
20	24.802230037085586	25.580215090809272	26.236705628942445	23.380849243162693
21	24.573963900373098	25.353717101946156	26.534839865326653	23.537479132354093
22	24.384789586904642	25.174906585773027	26.441653221740445	23.998650605581886
23	23.983892698284652	25.181471491154362	26.681210995708827	24.153424814852162
24	23.855098007910097	25.330468583689065	26.645270327047832	24.169163081353005
25	24.244265598915444	25.265362228720605	26.56390051314802	23.926471659215938
26	24.065980275172823	25.485592921246347	26.619080731179906	23.829346072400927
27	24.27547953570188	25.587445239935914	26.246509220978563	23.89056600338364
28	24.19344447805676	25.534400804454755	26.393493075862995	23.87866164162549
29	24.194319798774274	25.513673209864095	26.419069947228664	23.872937044132968
30	24.248327087044693	25.692763828666827	26.329192015954643	23.729717068333837
31	24.30739372906233	25.34120001568575	26.501105004873786	23.850301250378138
32	24.299130701489023	25.50120864284674	26.330382452130465	23.869278203533774
33	24.193269413913256	25.41035035236911	26.42530223073734	23.971078002980292
34	24.313468454841853	25.369455368446996	26.401616052121497	23.91546012458965
35	24.251408215970333	25.590491356032853	26.267569437441878	23.89053099055494
36	24.256450063303195	25.540860671349986	26.4339679058407	23.768721359506124
37	24.479866923240674	25.294370357298913	26.283517780914927	23.942244938545485
38	24.17012593414227	25.57911218670521	26.281732126651203	23.969029752501317
39	24.137826599666116	25.609170700144535	26.38022321378553	23.872779486403818
40	24.31635701320964	25.409597576552052	26.434510604685556	23.839534805552752
41	24.299060675831623	25.521866211779997	26.40762075224362	23.771452360144757
42	24.317197321098448	25.445363181069542	26.35531158616517	23.882127911666835
43	24.334353607161663	25.210094478616966	26.3136288135973	24.14192310062407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1003.0
1	1000.0
2	997.0
3	2040.0
4	3083.0
5	3083.0
6	3546.0
7	4009.0
8	3136.0
9	2263.0
10	3329.0
11	4395.0
12	4395.0
13	7977.5
14	11560.0
15	23682.0
16	35804.0
17	37806.0
18	39808.0
19	39808.0
20	39635.0
21	39462.0
22	36620.5
23	33779.0
24	41841.5
25	49904.0
26	49904.0
27	59936.5
28	69969.0
29	83800.5
30	97632.0
31	116970.0
32	136308.0
33	136308.0
34	158392.5
35	180477.0
36	206167.0
37	231857.0
38	260261.5
39	288666.0
40	288666.0
41	316641.0
42	344616.0
43	374488.5
44	404361.0
45	430181.5
46	456002.0
47	456002.0
48	476031.0
49	496060.0
50	502041.0
51	508022.0
52	498535.5
53	489049.0
54	489049.0
55	470842.5
56	452636.0
57	415996.5
58	379357.0
59	340510.0
60	301663.0
61	301663.0
62	263594.5
63	225526.0
64	191128.5
65	156731.0
66	129856.0
67	102981.0
68	102981.0
69	83079.0
70	63177.0
71	52471.5
72	41766.0
73	33824.0
74	25882.0
75	25882.0
76	20327.5
77	14773.0
78	11783.5
79	8794.0
80	6853.0
81	4912.0
82	4912.0
83	3784.0
84	2656.0
85	2021.5
86	1387.0
87	958.5
88	530.0
89	530.0
90	381.5
91	233.0
92	148.0
93	63.0
94	41.5
95	20.0
96	20.0
97	16.5
98	13.0
99	9.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5712192.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.01477874218254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.16434656244574	39.31806423566608
2	13.644207566491929	14.46689290499965
3	4.678826442578584	7.441408458791302
4	2.3315231771809075	4.944207414820651
5	1.3538510935113353	3.588705808618267
6	0.8510656853283721	2.7071435401648563
7	0.6026239463588154	2.236358262666744
8	0.39407677016364073	1.6713514222127472
9	0.2991286224914913	1.4272413963156234
>10	1.5686929058764472	14.514580954320527
>50	0.07826938245656664	2.8220842372933914
>100	0.03179240785217751	2.8589163522657364
>500	0.0011633423453039254	0.4190500232090867
>1k	2.9914263617973423E-4	0.2541842179131546
>5k	3.3238070672438295E-5	0.09980585163692567
>10k+	9.971421201731487E-5	1.2300049191052826
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26576	0.46525046777139145	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23527	0.411873410417577	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19700	0.344876362699293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.750641435021792E-5
2	0.0	0.0	0.0	0.0	3.501282870043584E-5
3	0.0	0.0	0.0	1.750641435021792E-5	3.501282870043584E-5
4	0.0	0.0	0.0	5.251924305065376E-5	3.501282870043584E-5
5	1.750641435021792E-5	0.0	0.0	5.251924305065376E-5	3.501282870043584E-5
6	1.0503848610130751E-4	0.0	0.0	7.002565740087168E-5	3.501282870043584E-5
7	1.0503848610130751E-4	0.0	0.0	1.0503848610130751E-4	3.501282870043584E-5
8	1.0503848610130751E-4	0.0	0.0	1.0503848610130751E-4	3.501282870043584E-5
9	1.0503848610130751E-4	0.0	0.0	2.1007697220261503E-4	3.501282870043584E-5
10	1.2254490045152545E-4	0.0	0.0	3.501282870043584E-4	3.501282870043584E-5
11	1.4005131480174335E-4	0.0	0.0	4.37660358755448E-4	3.501282870043584E-5
12	1.4005131480174335E-4	0.0	0.0	4.7267318745588383E-4	3.501282870043584E-5
13	1.4005131480174335E-4	0.0	0.0	4.901796018061018E-4	3.501282870043584E-5
14	1.4005131480174335E-4	0.0	0.0	5.076860161563197E-4	5.251924305065376E-5
15	1.4005131480174335E-4	1.750641435021792E-5	0.0	5.076860161563197E-4	8.75320717510896E-5
16	1.750641435021792E-4	1.750641435021792E-5	0.0	5.251924305065376E-4	8.75320717510896E-5
17	1.750641435021792E-4	1.750641435021792E-5	0.0	5.251924305065376E-4	8.75320717510896E-5
18	1.750641435021792E-4	1.750641435021792E-5	0.0	5.952180879074092E-4	8.75320717510896E-5
19	1.750641435021792E-4	1.750641435021792E-5	0.0	6.127245022576272E-4	8.75320717510896E-5
20	1.750641435021792E-4	1.750641435021792E-5	0.0	6.827501596584989E-4	8.75320717510896E-5
21	1.750641435021792E-4	1.750641435021792E-5	0.0	7.877886457598064E-4	8.75320717510896E-5
22	1.750641435021792E-4	1.750641435021792E-5	0.0	9.453463749117677E-4	8.75320717510896E-5
23	1.750641435021792E-4	1.750641435021792E-5	0.0	0.0011729297614646006	8.75320717510896E-5
24	1.750641435021792E-4	1.750641435021792E-5	0.0	0.0014005131480174335	8.75320717510896E-5
25	1.750641435021792E-4	1.750641435021792E-5	0.0	0.001540564462819177	8.75320717510896E-5
26	1.9257055785239712E-4	1.750641435021792E-5	0.0	0.0018381735067728815	8.75320717510896E-5
27	1.9257055785239712E-4	1.750641435021792E-5	0.0	0.0027485070529842132	8.75320717510896E-5
28	1.9257055785239712E-4	1.750641435021792E-5	0.0	0.005864648807323003	8.75320717510896E-5
29	1.9257055785239712E-4	1.750641435021792E-5	0.0	0.012412047774304505	8.75320717510896E-5
30	1.9257055785239712E-4	1.750641435021792E-5	0.0	0.02121777419246412	8.75320717510896E-5
31	2.1007697220261503E-4	1.750641435021792E-5	0.0	0.033034603878861216	8.75320717510896E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5205	0.0	28.46974	1
GTATCAA	10700	0.0	19.399065	1
TGCGAAC	625	0.0	16.576	12
GTGCGAA	645	0.0	16.348837	11
CCGTAGT	655	0.0	16.099237	20
GTCGAAA	640	0.0	15.8984375	25
TAGTCGA	745	0.0	14.899328	23
TCGAAAT	705	0.0	14.695036	26
TATACCG	200	9.858923E-10	13.875001	5
CGGGTTA	360	0.0	13.875	34
GTAGTCG	765	0.0	13.542483	22
CGTAGTC	780	0.0	13.51923	21
AGTCGAA	825	0.0	13.454546	24
GTATAGG	675	0.0	13.429629	1
TATCAAC	16070	0.0	12.824517	2
CCCCGTA	825	0.0	12.557576	18
CCCGTAG	825	0.0	12.557576	19
ATCAACG	16340	0.0	12.522032	3
TCAACGC	16455	0.0	12.412033	4
GTATTAT	690	0.0	12.333333	1
>>END_MODULE