FastQCFastQC Report
Thu 26 May 2016
SRR1041741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8914233
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT305530.3427440139830314No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC296220.3323000419665943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT290450.32582724727971546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT246270.27626605676562416No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC163940.18390813881575677No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA163640.1835715983640993No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC152930.17155710423992732No Hit
CTCTATGACCAGGCTTTCAATCCTGTTCGTCTATACAACGTTG152810.17142248805926433No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT144390.16197691938274442No Hit
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTA134510.15089352050815813No Hit
CTCCTACCCATCAACACGGCTGAACCAACACCACAAGGGTGCG128670.14434219971589257No Hit
ATCCACAGCTCATCCCCTCCATTTTTAACTGAAGTGGGTTCGG122130.13700561786975954No Hit
GTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTGCGA113080.12685331424475893No Hit
TATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGAC109690.1230504071410294No Hit
GGTTTACACCGCCGCTTCGCTGGCCACACGACGCTCTCCTACC106740.11974109269973086No Hit
GTATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGA102730.1152426686625759No Hit
CCACAAGGGTGCGGCTTACCAAAAATATCAATGCCACAACTTC99230.11131636339323865No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC98690.1107105905802552No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA96940.10874743794558658No Hit
GCCTAAATACTCCTACATGACCGATAGTGAACAAGTACCGTGA91850.10303746828246468No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCG91760.10293650614696744No Hit
GACTTACCCAGGGCGGATTAGCCTGGCCCTGGAAACCTTGATC91010.10209515501782374No Hit
CCCTTTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTG90000.10096213549724356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93350.029.2908421
TCGAAAT62350.019.04891626
TACGCAT34400.018.82267419
TCGTCTA31400.018.67675227
GTATCAA189150.018.661381
GTCGAAA63700.018.6161725
GTGCGAA65300.018.55666211
AGTCGAA64850.018.54279124
CTACGCA35400.018.4477418
CCGTAGT64600.018.35681220
CGCTACG35800.018.29329716
TGCGAAC65700.018.162112
CCCGTAG65750.018.1482919
GCTACGC36750.017.87074917
TAGTCGA67550.017.85640323
GTAGTCG67200.017.75669722
CCCCGTA67350.017.71714818
CGGGTTA36750.017.66938834
CGTAGTC67900.017.60088321
GTTCGTC33800.017.51479125
Produced by FastQC (version 0.11.5)