##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041741_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8914233
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55532943776542	33.0	31.0	34.0	31.0	34.0
2	32.81662572652072	34.0	31.0	34.0	31.0	34.0
3	32.873483001846594	34.0	31.0	34.0	31.0	34.0
4	36.33711930123433	37.0	37.0	37.0	35.0	37.0
5	36.28090223802766	37.0	37.0	37.0	35.0	37.0
6	36.36451290873819	37.0	37.0	37.0	35.0	37.0
7	36.32355885245539	37.0	37.0	37.0	35.0	37.0
8	36.30711974883313	37.0	37.0	37.0	35.0	37.0
9	38.019833787158134	39.0	38.0	39.0	35.0	39.0
10	37.94919529251703	39.0	38.0	39.0	35.0	39.0
11	38.04366197293699	39.0	38.0	39.0	35.0	39.0
12	37.89382159968222	39.0	38.0	39.0	35.0	39.0
13	37.93794833498294	39.0	38.0	39.0	35.0	39.0
14	38.83557609499326	40.0	39.0	40.0	36.0	40.0
15	38.862840583143836	40.0	39.0	40.0	36.0	40.0
16	38.839947979820586	40.0	39.0	40.0	36.0	40.0
17	38.8267735429397	40.0	39.0	40.0	36.0	40.0
18	38.76950882930702	40.0	39.0	40.0	36.0	40.0
19	38.7650981301476	40.0	39.0	40.0	36.0	40.0
20	38.69805141956689	40.0	39.0	40.0	36.0	40.0
21	38.639523557438984	40.0	39.0	40.0	36.0	40.0
22	38.572551671018694	40.0	38.0	40.0	36.0	40.0
23	38.50536709103296	40.0	38.0	40.0	35.0	40.0
24	38.44277045484451	40.0	38.0	40.0	35.0	40.0
25	38.41979237024655	40.0	38.0	40.0	35.0	40.0
26	38.26106261750169	40.0	38.0	40.0	35.0	40.0
27	38.20370344818225	40.0	38.0	40.0	35.0	40.0
28	38.10877817530684	40.0	38.0	40.0	34.0	40.0
29	38.03305825638616	40.0	38.0	40.0	34.0	40.0
30	37.940464760120136	40.0	38.0	40.0	34.0	40.0
31	37.84894303301249	40.0	38.0	40.0	34.0	40.0
32	37.954344810147994	40.0	38.0	40.0	34.0	40.0
33	38.07533940385	40.0	38.0	40.0	34.0	40.0
34	38.07835188961294	40.0	38.0	40.0	34.0	40.0
35	38.11778781191831	40.0	38.0	40.0	35.0	40.0
36	38.092441267801725	40.0	38.0	40.0	35.0	40.0
37	38.06013618894637	40.0	38.0	40.0	34.0	40.0
38	38.01366085001368	40.0	38.0	40.0	34.0	40.0
39	37.938803372090455	40.0	38.0	40.0	34.0	40.0
40	37.89849839015875	40.0	38.0	40.0	34.0	40.0
41	37.8421744192686	40.0	38.0	40.0	34.0	40.0
42	37.743248577864186	40.0	38.0	40.0	33.0	40.0
43	37.35507530485236	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	5.0
13	1.0
14	4.0
15	10.0
16	21.0
17	38.0
18	81.0
19	152.0
20	398.0
21	938.0
22	1934.0
23	3835.0
24	7003.0
25	11505.0
26	17948.0
27	26733.0
28	38871.0
29	54433.0
30	72863.0
31	96390.0
32	126767.0
33	167857.0
34	235298.0
35	346613.0
36	591970.0
37	1309496.0
38	4831138.0
39	971929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42540743550231	19.014995457264803	13.087519700236689	24.472077406996203
2	18.67940853688702	19.56760609690144	36.61337997335273	25.139605392858815
3	19.767612087321478	21.917746596930996	29.677023250345826	28.6376180654017
4	14.586246511617992	16.039450617905096	34.94233323270774	34.43196963776917
5	14.989657551019814	36.34731109227232	33.607647455479345	15.05538390122852
6	34.115890845572466	35.46514882435763	15.219840001938472	15.199120328131427
7	30.334713037004978	30.849844288342027	18.846680359375842	19.968762315277154
8	27.874669643479145	34.57646888969584	17.068265996637063	20.480595470187957
9	27.194083888092223	14.744308343746457	18.519843490741156	39.541764277420164
10	17.471396585662504	27.40374858947483	29.877612577548735	25.247242247313928
11	37.43609797948965	22.072745910949376	20.417718495803282	20.073437613757683
12	23.783885837401826	23.736871136305275	28.05411301230291	24.42513001398999
13	30.51606346838814	20.299648887346784	24.543435200762644	24.640852443502432
14	22.98050768922015	19.71953167479468	25.841639993031368	31.4583206429538
15	25.992544731554585	27.238821332132556	22.32056308153489	24.44807085477797
16	25.055975090621928	27.016121297255747	23.15803277746947	24.769870834652853
17	24.431490628526312	26.327525879119378	25.168884412153012	24.07209908020129
18	25.13607171811641	25.427448441161456	25.537665439079277	23.898814401642856
19	26.981031346162926	24.745550178013072	25.35483422970883	22.918584246115174
20	25.541434692137845	25.30599099215827	24.878977249080204	24.27359706662368
21	25.95806055327475	24.808741256819292	25.41638747831698	23.816810711588985
22	24.849350471319294	24.924836494626064	25.38147701546504	24.844336018589598
23	24.339895535600203	24.483272986021344	26.594335149193434	24.582496329185023
24	23.89553874124672	25.248543537060343	26.355671878893	24.500245842799938
25	24.31409410097313	25.517630064190605	26.190105194692574	23.978170640143688
26	24.044861739647146	25.574628798686327	26.406197818701845	23.974311642964683
27	24.68666681698807	25.9561422727003	25.236506606906055	24.120684303405575
28	24.94081094806474	25.229450475436305	25.457277143193362	24.372461433305592
29	24.952948840354523	24.997450706078695	25.194730718840308	24.854869734726474
30	24.040195045384163	26.196039524656804	25.641499386430667	24.122266043528366
31	24.246718702551302	25.126671021500112	26.033333434295468	24.59327684165312
32	24.885932418414463	25.09232145940094	25.667973901961055	24.353772220223547
33	25.633097093154284	25.347879060374574	25.589178564212983	23.42984528225816
34	25.169153644514342	24.85637294874388	24.847454626774955	25.127018779966825
35	25.039753840852043	26.33699388382601	24.713904157542213	23.909348117779736
36	25.041750647531874	25.07028927783243	25.797990696451397	24.089969378184303
37	25.83392199867336	24.54087749333005	25.102170876619446	24.523029631377145
38	24.989732711720684	25.18599188511227	25.36445928662623	24.459816116540818
39	24.563863206178254	24.737675131444288	26.23882503407752	24.459636628299933
40	24.860119765772332	25.066609768894306	26.270201822187055	23.803068643146304
41	25.979430871954996	24.723461906369288	25.493107483279832	23.80399973839589
42	25.776698903876532	24.67084941576017	25.32030517936877	24.232146500994535
43	25.27102443923106	23.98326361897877	25.609931892065198	25.135780049724975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2151.0
1	1746.0
2	1341.0
3	2364.5
4	3388.0
5	3388.0
6	4081.0
7	4774.0
8	3882.0
9	2990.0
10	4020.5
11	5051.0
12	5051.0
13	9522.5
14	13994.0
15	29031.5
16	44069.0
17	45113.0
18	46157.0
19	46157.0
20	47334.5
21	48512.0
22	43699.5
23	38887.0
24	47544.5
25	56202.0
26	56202.0
27	68259.5
28	80317.0
29	96774.5
30	113232.0
31	135506.5
32	157781.0
33	157781.0
34	182453.5
35	207126.0
36	239487.0
37	271848.0
38	318029.0
39	364210.0
40	364210.0
41	411680.5
42	459151.0
43	519724.5
44	580298.0
45	660958.0
46	741618.0
47	741618.0
48	810202.0
49	878786.0
50	916631.5
51	954477.0
52	951842.0
53	949207.0
54	949207.0
55	912102.0
56	874997.0
57	768837.0
58	662677.0
59	553337.5
60	443998.0
61	443998.0
62	381929.5
63	319861.0
64	287287.0
65	254713.0
66	197264.5
67	139816.0
68	139816.0
69	109265.5
70	78715.0
71	64179.5
72	49644.0
73	39233.0
74	28822.0
75	28822.0
76	22356.0
77	15890.0
78	12560.5
79	9231.0
80	7210.5
81	5190.0
82	5190.0
83	3989.5
84	2789.0
85	2106.5
86	1424.0
87	1006.0
88	588.0
89	588.0
90	401.0
91	214.0
92	138.5
93	63.0
94	41.0
95	19.0
96	19.0
97	12.0
98	5.0
99	7.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8914233.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.29193036984744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.81813377835606	27.88346908303046
2	13.937382193008114	10.673785369452366
3	4.841414828952518	5.5616135856539
4	2.4496364235549897	3.7520522944883914
5	1.438068953873772	2.753321812438692
6	0.8981218553681604	2.063449172963747
7	0.6621104542900756	1.7747441188976543
8	0.47790021061818344	1.4639777270981524
9	0.3610605776518723	1.244313584886815
>10	1.9073440324883384	13.088058736857613
>50	0.11886893704631464	3.0960434180389833
>100	0.0634019172457202	4.838371771210529
>500	0.010652074289442311	2.913913986532984
>1k	0.013556344342221157	10.94938479256386
>5k	0.001877935131592307	4.871908799741458
>10k+	4.6948378289807675E-4	3.0715917461444406
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30553	0.3427440139830314	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	29622	0.3323000419665943	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29045	0.32582724727971546	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24627	0.27626605676562416	No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC	16394	0.18390813881575677	No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA	16364	0.1835715983640993	No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC	15293	0.17155710423992732	No Hit
CTCTATGACCAGGCTTTCAATCCTGTTCGTCTATACAACGTTG	15281	0.17142248805926433	No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT	14439	0.16197691938274442	No Hit
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTA	13451	0.15089352050815813	No Hit
CTCCTACCCATCAACACGGCTGAACCAACACCACAAGGGTGCG	12867	0.14434219971589257	No Hit
ATCCACAGCTCATCCCCTCCATTTTTAACTGAAGTGGGTTCGG	12213	0.13700561786975954	No Hit
GTATAGACGAACAGGATTGAAAGCCTGGTCATAGAGGGTGCGA	11308	0.12685331424475893	No Hit
TATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGAC	10969	0.1230504071410294	No Hit
GGTTTACACCGCCGCTTCGCTGGCCACACGACGCTCTCCTACC	10674	0.11974109269973086	No Hit
GTATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGA	10273	0.1152426686625759	No Hit
CCACAAGGGTGCGGCTTACCAAAAATATCAATGCCACAACTTC	9923	0.11131636339323865	No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC	9869	0.1107105905802552	No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA	9694	0.10874743794558658	No Hit
GCCTAAATACTCCTACATGACCGATAGTGAACAAGTACCGTGA	9185	0.10303746828246468	No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCG	9176	0.10293650614696744	No Hit
GACTTACCCAGGGCGGATTAGCCTGGCCCTGGAAACCTTGATC	9101	0.10209515501782374	No Hit
CCCTTTGTTGGTGTGACAGCGTGCCTTTTGAAGAATGAGCCTG	9000	0.10096213549724356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1218015055249285E-5	2.243603011049857E-5
2	0.0	0.0	0.0	2.243603011049857E-5	2.243603011049857E-5
3	0.0	0.0	0.0	2.243603011049857E-5	2.243603011049857E-5
4	0.0	0.0	0.0	4.487206022099714E-5	3.365404516574786E-5
5	0.0	1.1218015055249285E-5	0.0	5.6090075276246425E-5	3.365404516574786E-5
6	5.6090075276246425E-5	1.1218015055249285E-5	0.0	5.6090075276246425E-5	3.365404516574786E-5
7	5.6090075276246425E-5	1.1218015055249285E-5	0.0	6.730809033149571E-5	3.365404516574786E-5
8	5.6090075276246425E-5	1.1218015055249285E-5	0.0	6.730809033149571E-5	3.365404516574786E-5
9	5.6090075276246425E-5	1.1218015055249285E-5	0.0	8.974412044199428E-5	4.487206022099714E-5
10	5.6090075276246425E-5	1.1218015055249285E-5	0.0	1.1218015055249285E-4	4.487206022099714E-5
11	7.8526105386745E-5	1.1218015055249285E-5	0.0	1.2339816560774213E-4	4.487206022099714E-5
12	7.8526105386745E-5	1.1218015055249285E-5	0.0	1.458341957182407E-4	5.6090075276246425E-5
13	7.8526105386745E-5	1.1218015055249285E-5	0.0	1.5705221077349E-4	6.730809033149571E-5
14	7.8526105386745E-5	1.1218015055249285E-5	0.0	1.6827022582873927E-4	1.2339816560774213E-4
15	8.974412044199428E-5	3.365404516574786E-5	0.0	1.6827022582873927E-4	1.7948824088398856E-4
16	1.3461618066299143E-4	3.365404516574786E-5	0.0	1.7948824088398856E-4	2.0192427099448713E-4
17	1.3461618066299143E-4	3.365404516574786E-5	0.0	1.9070625593923783E-4	2.0192427099448713E-4
18	1.3461618066299143E-4	3.365404516574786E-5	0.0	2.1314228604973643E-4	2.243603011049857E-4
19	1.3461618066299143E-4	3.365404516574786E-5	0.0	2.35578316160235E-4	2.243603011049857E-4
20	1.6827022582873927E-4	3.365404516574786E-5	0.0	2.916683914364814E-4	2.35578316160235E-4
21	1.9070625593923783E-4	3.365404516574786E-5	0.0	3.92630526933725E-4	2.35578316160235E-4
22	1.9070625593923783E-4	3.365404516574786E-5	0.0	4.935926624309685E-4	2.35578316160235E-4
23	1.9070625593923783E-4	3.365404516574786E-5	0.0	5.833367828729628E-4	3.1410442154698E-4
24	2.0192427099448713E-4	3.365404516574786E-5	0.0	7.964790689226993E-4	3.2532243660222926E-4
25	2.0192427099448713E-4	3.365404516574786E-5	0.0	8.974412044199428E-4	3.3654045165747853E-4
26	2.1314228604973643E-4	3.365404516574786E-5	0.0	0.0010432754001381834	3.4775846671272786E-4
27	2.1314228604973643E-4	3.365404516574786E-5	0.0	0.0017051382883978914	3.701944968232264E-4
28	2.243603011049857E-4	3.365404516574786E-5	0.0	0.0037131629832875134	3.701944968232264E-4
29	2.243603011049857E-4	3.365404516574786E-5	0.0	0.007459980011740775	3.8141251187847567E-4
30	2.243603011049857E-4	3.365404516574786E-5	0.0	0.01349527211146489	3.8141251187847567E-4
31	2.243603011049857E-4	3.365404516574786E-5	0.0	0.022110707673896342	3.92630526933725E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9335	0.0	29.290842	1
TCGAAAT	6235	0.0	19.048916	26
TACGCAT	3440	0.0	18.822674	19
TCGTCTA	3140	0.0	18.676752	27
GTATCAA	18915	0.0	18.66138	1
GTCGAAA	6370	0.0	18.61617	25
GTGCGAA	6530	0.0	18.556662	11
AGTCGAA	6485	0.0	18.542791	24
CTACGCA	3540	0.0	18.44774	18
CCGTAGT	6460	0.0	18.356812	20
CGCTACG	3580	0.0	18.293297	16
TGCGAAC	6570	0.0	18.1621	12
CCCGTAG	6575	0.0	18.14829	19
GCTACGC	3675	0.0	17.870749	17
TAGTCGA	6755	0.0	17.856403	23
GTAGTCG	6720	0.0	17.756697	22
CCCCGTA	6735	0.0	17.717148	18
CGGGTTA	3675	0.0	17.669388	34
CGTAGTC	6790	0.0	17.600883	21
GTTCGTC	3380	0.0	17.514791	25
>>END_MODULE