FastQCFastQC Report
Thu 26 May 2016
SRR1041740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7512614
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT389240.5181152658715062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT360210.479473589352521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT314460.4185760109597006No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC168360.22410308848557903No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC110600.14721906383051225No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC97670.13000801052736105No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91490.12178184583954399No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA90520.12049068406815523No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT79390.10567560106242647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92900.030.6076451
TACGCAT20900.020.88995219
CTACGCA22550.019.44345918
GCTACGC23050.019.0216917
ACGCATT23650.018.61733820
CGCATTC23900.018.57740421
CGCTACG23400.018.516
TCGAAAT37650.018.32802226
GTATCAA185350.018.3253291
GTCGAAA39000.017.6935925
GTGCGAA40150.017.46326411
CCCGTAG40450.017.33374619
CGGGTTA25000.017.31634
CCGTAGT40300.017.26054620
TGCGAAC40200.017.21144312
AGTCGAA40950.017.07692324
GCTTTCG26000.017.0057711
TCGGGTT26000.016.8634633
TAGTCGA42000.016.73809423
CGTAGTC41900.016.60143321
Produced by FastQC (version 0.11.5)