##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041740_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7512614
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.721057677128094	31.0	31.0	34.0	30.0	34.0
2	31.990584102950052	31.0	31.0	34.0	30.0	34.0
3	32.0393530134784	33.0	31.0	34.0	30.0	34.0
4	35.75713007483148	37.0	35.0	37.0	35.0	37.0
5	35.61399853632837	37.0	35.0	37.0	33.0	37.0
6	35.78834956780689	37.0	35.0	37.0	35.0	37.0
7	35.75285872533848	37.0	35.0	37.0	35.0	37.0
8	35.736219643389106	37.0	35.0	37.0	33.0	37.0
9	37.33751341410593	39.0	37.0	39.0	34.0	39.0
10	37.13063415743176	39.0	37.0	39.0	33.0	39.0
11	37.324400268668136	39.0	37.0	39.0	34.0	39.0
12	37.08815426960576	39.0	37.0	39.0	33.0	39.0
13	37.16434692372056	39.0	37.0	39.0	34.0	39.0
14	37.964525396885826	40.0	38.0	40.0	34.0	40.0
15	37.99064173934665	40.0	38.0	40.0	34.0	40.0
16	37.93886016238822	40.0	38.0	40.0	34.0	40.0
17	37.91337582364807	40.0	38.0	40.0	33.0	40.0
18	37.83998113040281	40.0	38.0	40.0	33.0	40.0
19	37.843548996394595	40.0	38.0	40.0	34.0	40.0
20	37.75103951833543	40.0	37.0	40.0	33.0	40.0
21	37.67269009162457	39.0	37.0	40.0	33.0	40.0
22	37.57176330369163	39.0	37.0	40.0	33.0	40.0
23	37.475483633260005	39.0	37.0	40.0	33.0	40.0
24	37.372591084807496	39.0	37.0	40.0	32.0	40.0
25	37.333003532458875	39.0	37.0	40.0	32.0	40.0
26	37.11325924638215	39.0	36.0	40.0	32.0	40.0
27	37.018867201216516	39.0	36.0	40.0	32.0	40.0
28	36.88910930336631	39.0	36.0	40.0	31.0	40.0
29	36.782077583115544	39.0	36.0	40.0	31.0	40.0
30	36.64775216722169	38.0	36.0	40.0	31.0	40.0
31	36.51148455118285	38.0	35.0	40.0	30.0	40.0
32	36.60471455075424	38.0	35.0	40.0	31.0	40.0
33	36.765437170071564	39.0	36.0	40.0	31.0	40.0
34	36.7702059496202	39.0	36.0	40.0	31.0	40.0
35	36.80692166534844	39.0	36.0	40.0	31.0	40.0
36	36.76565200874156	39.0	36.0	40.0	31.0	40.0
37	36.71728681920833	39.0	36.0	40.0	31.0	40.0
38	36.64163765102267	39.0	35.0	40.0	31.0	40.0
39	36.53160311444192	39.0	35.0	40.0	30.0	40.0
40	36.48550025330731	38.0	35.0	40.0	30.0	40.0
41	36.396223471617205	38.0	35.0	40.0	30.0	40.0
42	36.27033293604596	38.0	35.0	40.0	30.0	40.0
43	35.73849927601764	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	5.0
14	9.0
15	28.0
16	48.0
17	100.0
18	184.0
19	486.0
20	984.0
21	2164.0
22	4135.0
23	7521.0
24	12482.0
25	20058.0
26	30202.0
27	43932.0
28	62299.0
29	84979.0
30	115061.0
31	153196.0
32	204178.0
33	279665.0
34	400714.0
35	612457.0
36	1014962.0
37	1713153.0
38	2531862.0
39	217745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.85589729486967	21.569749224437725	14.422649692903164	22.151703787789444
2	20.393247942726724	22.289378903268556	34.46623239261328	22.85114076139144
3	21.571892286759308	23.926239255737084	30.009022159264404	24.4928462982392
4	16.484009427344464	18.280494645405714	34.18877903217176	31.046716895078063
5	15.003778977596879	37.47060610328176	33.30402706701023	14.221587852111128
6	33.64038136393005	36.80552468155558	14.967080699208024	14.587013255306342
7	29.474374698340682	31.822106127108352	19.463917619087045	19.239601555463917
8	27.878698413095627	34.98542584511862	17.47197446854051	19.663901273245237
9	27.382027081386052	14.59569199216145	18.518813824322667	39.50346710212983
10	16.88413646701401	28.60337294049714	30.64571133296613	23.866779259522716
11	37.24800715170512	22.34179740899772	20.741688578702433	19.66850686059473
12	23.502245689715988	24.869173366287686	28.47198059157571	23.156600352420607
13	30.584733888896725	20.880641545006835	24.69360198727101	23.841022578825427
14	22.814695390978425	20.40426940609487	26.49428015335275	30.286755049573955
15	25.76243102600506	28.079440791181337	22.535005791592646	23.623122391220953
16	24.65269744991557	27.609404662611443	23.698941540188276	24.038956347284714
17	24.235372667888967	26.982246126315022	25.451633745591085	23.33074746020493
18	24.72163483975085	25.85647286017889	26.122598605492044	23.299293694578214
19	26.64287024463123	25.330650556517348	26.06122183303974	21.965257365811684
20	25.31212171955061	25.605148886925377	25.96899028753507	23.11373910598894
21	25.559878359250188	25.29371268109875	26.0327630302848	23.113645929366264
22	24.494816318261524	25.424918676774823	26.275927393580982	23.80433761138267
23	24.192218580643168	25.032125968404607	27.09019789916	23.685457551792226
24	23.697317604764468	25.64462915304846	26.936003899574768	23.722049342612305
25	24.203519573879344	25.74390218903833	27.04394236147365	23.008635875608675
26	23.899777094896663	25.89809618862356	27.02344350448459	23.178683211995185
27	24.489798091583037	26.17665435759111	26.015977927256746	23.317569623569106
28	24.677974936553376	25.740148502239034	26.22514613422172	23.356730426985866
29	24.687452330174292	25.558933282077316	26.15351993327489	23.600094454473503
30	24.173104062048175	26.333790076263735	26.13464501170964	23.35846084997845
31	24.21978555000962	25.675683590292277	26.622650917510203	23.4818799421879
32	24.636191876755547	25.696249002011818	26.314369405908515	23.35318971532412
33	25.201734043569925	25.54486361205301	26.283354901502992	22.97004744287408
34	24.887116521626158	25.36841371059394	25.904432198965633	23.840037568814264
35	24.7898800603891	26.345024514769428	25.699749780835273	23.165345644006198
36	24.54378728895162	25.645614163059623	26.55535077404483	23.25524777394393
37	25.174246939880046	25.23410626447732	25.987851898154226	23.603794897488413
38	24.58025928125683	25.502308517381568	26.266103915361548	23.651328286000055
39	24.24704636761585	25.45619940010228	26.787041101805574	23.50971313047629
40	24.580685231531927	25.627684318667242	26.639502575268743	23.152127874532084
41	25.266957679444197	25.29387241245191	26.278589582800343	23.160580325303552
42	25.275370197377374	25.37923550977063	25.96809845414659	23.377295838705408
43	24.813054417543615	24.7164435707731	26.31757734391784	24.15292466776544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4403.0
1	3354.0
2	2305.0
3	3182.0
4	4059.0
5	4059.0
6	5205.0
7	6351.0
8	5179.0
9	4007.0
10	5515.0
11	7023.0
12	7023.0
13	12806.5
14	18590.0
15	38660.0
16	58730.0
17	57677.0
18	56624.0
19	56624.0
20	59152.5
21	61681.0
22	55121.5
23	48562.0
24	58245.5
25	67929.0
26	67929.0
27	80365.0
28	92801.0
29	108517.5
30	124234.0
31	145942.0
32	167650.0
33	167650.0
34	191858.0
35	216066.0
36	244268.0
37	272470.0
38	309379.5
39	346289.0
40	346289.0
41	383246.5
42	420204.0
43	461143.0
44	502082.0
45	558621.0
46	615160.0
47	615160.0
48	664348.0
49	713536.0
50	728183.0
51	742830.0
52	734478.0
53	726126.0
54	726126.0
55	691833.5
56	657541.0
57	577349.5
58	497158.0
59	427021.0
60	356884.0
61	356884.0
62	304876.0
63	252868.0
64	221061.0
65	189254.0
66	150487.0
67	111720.0
68	111720.0
69	89203.0
70	66686.0
71	55340.0
72	43994.0
73	34523.5
74	25053.0
75	25053.0
76	19564.0
77	14075.0
78	11046.5
79	8018.0
80	6309.0
81	4600.0
82	4600.0
83	3640.0
84	2680.0
85	2061.5
86	1443.0
87	1018.0
88	593.0
89	593.0
90	411.0
91	229.0
92	145.5
93	62.0
94	48.0
95	34.0
96	34.0
97	20.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7512614.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.62572046520217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.89522661386154	35.852953412062824
2	12.451003500159644	11.610740174193946
3	4.128825164698628	5.775283439367916
4	1.9993682159500086	3.7288793417558024
5	1.1343031604230438	2.6443851040340105
6	0.723794072466704	2.0248452058321695
7	0.48928458614756093	1.5969272439153814
8	0.3587874467920597	1.3382978560440137
9	0.2594034484336662	1.0885385406939845
>10	1.407075329589195	11.679989624188645
>50	0.08338809114000892	2.678845730794651
>100	0.05076148684580718	4.868509532526041
>500	0.008702560698707458	2.8427384462638776
>1k	0.009332048952198394	8.650083180525952
>5k	6.011442563153593E-4	1.8266492038536597
>10k+	1.431295848369903E-4	1.7923339639472766
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38924	0.5181152658715062	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	36021	0.479473589352521	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31446	0.4185760109597006	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	16836	0.22410308848557903	No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC	11060	0.14721906383051225	No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC	9767	0.13000801052736105	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9149	0.12178184583954399	No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA	9052	0.12049068406815523	No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT	7939	0.10567560106242647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.3310946096791343E-5	0.0	0.0	1.3310946096791343E-5	0.0
6	5.324378438716537E-5	0.0	0.0	1.3310946096791343E-5	0.0
7	5.324378438716537E-5	0.0	0.0	2.6621892193582687E-5	0.0
8	6.655473048395672E-5	0.0	0.0	3.993283829037403E-5	0.0
9	6.655473048395672E-5	0.0	0.0	1.3310946096791343E-4	0.0
10	7.986567658074806E-5	0.0	0.0	1.5973135316149612E-4	0.0
11	7.986567658074806E-5	0.0	0.0	1.863532453550788E-4	0.0
12	7.986567658074806E-5	0.0	0.0	1.9966419145187015E-4	0.0
13	7.986567658074806E-5	0.0	0.0	1.9966419145187015E-4	2.6621892193582687E-5
14	7.986567658074806E-5	0.0	0.0	1.9966419145187015E-4	2.6621892193582687E-5
15	7.986567658074806E-5	0.0	0.0	1.9966419145187015E-4	3.993283829037403E-5
16	1.1979851487112209E-4	0.0	0.0	2.3959702974224418E-4	7.986567658074806E-5
17	1.1979851487112209E-4	0.0	0.0	2.3959702974224418E-4	7.986567658074806E-5
18	1.1979851487112209E-4	0.0	0.0	2.529079758390355E-4	7.986567658074806E-5
19	1.1979851487112209E-4	0.0	0.0	2.529079758390355E-4	7.986567658074806E-5
20	1.3310946096791343E-4	0.0	0.0	2.6621892193582687E-4	7.986567658074806E-5
21	1.5973135316149612E-4	0.0	0.0	3.727064907101576E-4	7.986567658074806E-5
22	1.5973135316149612E-4	0.0	0.0	4.7919405948448836E-4	1.0648756877433075E-4
23	1.5973135316149612E-4	0.0	0.0	5.324378438716537E-4	1.1979851487112209E-4
24	1.5973135316149612E-4	0.0	0.0	7.187910892267325E-4	1.1979851487112209E-4
25	1.5973135316149612E-4	0.0	0.0	7.986567658074806E-4	1.1979851487112209E-4
26	1.7304229925828746E-4	0.0	0.0	9.184552806786026E-4	1.1979851487112209E-4
27	1.7304229925828746E-4	0.0	0.0	0.001357716501872717	1.1979851487112209E-4
28	1.7304229925828746E-4	0.0	0.0	0.0028485424647133476	1.1979851487112209E-4
29	1.7304229925828746E-4	0.0	0.0	0.006322699395975888	1.1979851487112209E-4
30	1.7304229925828746E-4	0.0	0.0	0.011127950936917564	1.1979851487112209E-4
31	1.7304229925828746E-4	0.0	0.0	0.01834248372137847	1.1979851487112209E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9290	0.0	30.607645	1
TACGCAT	2090	0.0	20.889952	19
CTACGCA	2255	0.0	19.443459	18
GCTACGC	2305	0.0	19.02169	17
ACGCATT	2365	0.0	18.617338	20
CGCATTC	2390	0.0	18.577404	21
CGCTACG	2340	0.0	18.5	16
TCGAAAT	3765	0.0	18.328022	26
GTATCAA	18535	0.0	18.325329	1
GTCGAAA	3900	0.0	17.69359	25
GTGCGAA	4015	0.0	17.463264	11
CCCGTAG	4045	0.0	17.333746	19
CGGGTTA	2500	0.0	17.316	34
CCGTAGT	4030	0.0	17.260546	20
TGCGAAC	4020	0.0	17.211443	12
AGTCGAA	4095	0.0	17.076923	24
GCTTTCG	2600	0.0	17.00577	11
TCGGGTT	2600	0.0	16.86346	33
TAGTCGA	4200	0.0	16.738094	23
CGTAGTC	4190	0.0	16.601433	21
>>END_MODULE