FastQCFastQC Report
Thu 26 May 2016
SRR1041739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5079602
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT260220.5122842301424403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT216490.42619480817591615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214910.42308432826036374No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC135540.26683192895821367No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC116580.2295061699715844No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC95020.1870618997315144No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT75460.14855494584024495No Hit
ATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGG72050.14184182146554No Hit
AGGCTAAACCGATCATGTGTGATAGCCGGCAGGCGTTGCATGG71290.1403456412529958No Hit
ATCCACAGCTCATCCCCTCCATTTTTAACTGAAGTGGGTTCGG70230.13825886358813153No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC69370.13656581755814728No Hit
TATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGAC69050.13593584694233918No Hit
GGTAGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAG64860.127687169191602No Hit
GCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCT63330.1246751221847696No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62950.12392703207849749No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA62860.12374985284280147No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGT62620.12327737488094541No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA61970.12199774706758522No Hit
GGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTC59330.11680048948716848No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCG58160.11449715942312017No Hit
ATGTAACGGGGCTCAAGCACGCCACCGAAGTTGTGGCATTGAT56590.11140636608931172No Hit
GAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGCGAAGT52000.10237022506881445No Hit
GGTTTACACCGCCGCTTCGCTGGCCACACGACGCTCTCCTACC51050.10049999980313418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67600.030.815091
TACGCAT20950.021.81145719
CTACGCA21550.021.20417818
CGCTACG22000.020.77045416
ACGCATT22900.020.11571920
CGCATTC22850.020.07877321
GCTACGC22850.019.9978117
GCTTTCG24800.018.64919311
CGTATCG902.155166E-618.510
CGGGTTA25250.018.39009934
TCGGGTT25750.018.03300933
CGCTTTC25750.018.03300910
ATTCCCC27100.017.27121724
CTCGGGT27750.016.86666732
CTCTCGG28150.016.69271930
GTATCAA124800.016.6173882
TTCGCTA28050.016.48841314
TCGCTAC28800.016.12326415
GCATTCC29100.015.89347122
CCCCTCT29650.015.84822927
Produced by FastQC (version 0.11.5)