##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041739_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5079602
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.179535522664963	31.0	31.0	33.0	30.0	34.0
2	31.402474445832567	31.0	31.0	34.0	30.0	34.0
3	31.37428680435987	31.0	31.0	34.0	28.0	34.0
4	35.09409989207816	35.0	35.0	37.0	32.0	37.0
5	34.85346115699616	35.0	35.0	37.0	32.0	37.0
6	35.18002040317332	36.0	35.0	37.0	32.0	37.0
7	35.1721806157254	37.0	35.0	37.0	32.0	37.0
8	35.16418904473225	37.0	35.0	37.0	32.0	37.0
9	36.699445547111765	38.0	35.0	39.0	32.0	39.0
10	36.364657506631424	38.0	35.0	39.0	32.0	39.0
11	36.66572459810828	38.0	35.0	39.0	32.0	39.0
12	36.33508412666977	38.0	35.0	39.0	32.0	39.0
13	36.462163964814565	38.0	35.0	39.0	32.0	39.0
14	37.157416270014856	39.0	36.0	40.0	32.0	40.0
15	37.18796512010193	39.0	36.0	40.0	32.0	40.0
16	37.11012988812903	39.0	36.0	40.0	32.0	40.0
17	37.07173298222971	39.0	36.0	40.0	32.0	40.0
18	36.97307033110074	38.0	36.0	40.0	32.0	40.0
19	36.9811939990574	39.0	36.0	40.0	32.0	40.0
20	36.868419415536884	39.0	36.0	40.0	31.0	40.0
21	36.77336905529213	39.0	36.0	40.0	31.0	40.0
22	36.64778244437261	38.0	36.0	40.0	31.0	40.0
23	36.520084644426866	38.0	36.0	40.0	31.0	40.0
24	36.39570324604172	38.0	35.0	40.0	30.0	40.0
25	36.35548434700199	38.0	35.0	40.0	30.0	40.0
26	36.058865635536016	38.0	35.0	40.0	30.0	40.0
27	35.95639028412069	38.0	35.0	40.0	30.0	40.0
28	35.80934214924713	38.0	34.0	40.0	29.0	40.0
29	35.6665815550116	38.0	34.0	40.0	29.0	40.0
30	35.509499169423115	38.0	34.0	40.0	28.0	40.0
31	35.35350722359744	38.0	34.0	40.0	27.0	40.0
32	35.425356553525255	38.0	34.0	40.0	28.0	40.0
33	35.60046968246725	38.0	34.0	40.0	29.0	40.0
34	35.59587955906782	38.0	34.0	40.0	29.0	40.0
35	35.625153702986964	38.0	34.0	40.0	29.0	40.0
36	35.53978953469189	38.0	34.0	40.0	28.0	40.0
37	35.48786834086608	38.0	34.0	40.0	28.0	40.0
38	35.39547409423022	38.0	34.0	40.0	27.0	40.0
39	35.24102892313217	38.0	34.0	40.0	27.0	40.0
40	35.18836200158989	38.0	34.0	40.0	27.0	40.0
41	35.09115596064416	38.0	33.0	40.0	27.0	40.0
42	34.92643951238699	38.0	33.0	40.0	26.0	40.0
43	34.32694766243497	37.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	7.0
13	10.0
14	23.0
15	37.0
16	93.0
17	209.0
18	402.0
19	794.0
20	1648.0
21	3067.0
22	5694.0
23	9652.0
24	15961.0
25	24405.0
26	36553.0
27	52030.0
28	72604.0
29	98609.0
30	132436.0
31	175596.0
32	233199.0
33	308124.0
34	418494.0
35	574190.0
36	785928.0
37	979750.0
38	1051486.0
39	98597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.586742425882974	24.284619149295555	13.985249238030853	20.14338918679062
2	23.255818073935714	23.7681219906599	32.29152598963462	20.68453394576977
3	24.45250631840841	23.9939664564271	28.91708838605859	22.6364388391059
4	19.553677630649016	19.374293497797662	32.03325772373505	29.038771147818277
5	15.291847668380317	38.332747329416755	32.70366457844532	13.671740423757608
6	33.846943126646536	37.41696691984923	14.368940716221468	14.367149237282764
7	29.53528642598377	32.09798720450933	19.13246352765433	19.23426284185257
8	28.072356850005175	35.46012069449536	16.944555892371095	19.52296656312837
9	27.857733735832056	14.456171959929144	18.242078808536576	39.444015495702224
10	17.26009242456397	28.732664488280772	30.065564191840227	23.94167889531503
11	38.182735576527456	22.37622160161367	20.13484521031372	19.306197611545155
12	23.795899757500685	25.021606023464045	27.875116987512015	23.307377231523258
13	31.198566344371077	20.735738744885918	24.438548531951913	23.627146378791096
14	23.059464107621032	20.487589382002763	26.33082276918546	30.122123741190748
15	26.537335011680046	27.914135792528626	21.960066950127192	23.58846224566413
16	25.199710528502035	27.732920807575084	23.018653823665712	24.048714840257173
17	24.982193486812548	27.008316793323573	24.98937908914911	23.02011063071477
18	25.298261556712514	25.57424774618169	25.642973603050002	23.484517094055793
19	27.227802493187458	25.269834132674173	25.651714445344343	21.85064892879403
20	25.840922182485947	25.778436972030487	25.290327864269678	23.09031298121388
21	26.413565472255502	25.315428255993282	25.14047360403433	23.13053266771688
22	24.847596327428803	25.562947648260632	25.85468310312501	23.734772921185556
23	24.467133448644205	25.1031478450477	26.825566254993994	23.604152451314096
24	24.109979482644505	25.939965375240025	26.473137068612857	23.47691807350261
25	24.626693193679348	26.07649181963469	26.828834227563497	22.467980759122465
26	24.252845006360737	26.2134907419912	26.575841965571318	22.95782228607674
27	25.120334230910217	26.31676654982024	25.27875215420421	23.284147065065333
28	25.453096522129098	25.915219341987815	25.43545734488647	23.19622679099662
29	25.28950102783643	25.454218657288504	25.628878010521294	23.62740230435377
30	24.361869296059023	26.68403941883636	25.365412487041304	23.588678798063313
31	24.56704285099502	25.846670664355198	26.079621986131983	23.5066644985178
32	25.227645787996778	25.760266257080772	25.734870566631006	23.277217388291447
33	26.08025195674779	25.592438147713146	25.69864725622204	22.628662639317017
34	25.687150292483548	25.244025024007787	25.09393452479151	23.97489015871716
35	25.572948431786585	26.597083787273096	24.970243731693937	22.859724049246378
36	25.063912487631907	25.803753916153273	26.09105989012525	23.04127370608957
37	25.729968607776748	25.189217580432484	25.41362492573237	23.667188886058398
38	25.014420421127483	25.501584572964575	25.715243044632235	23.768751961275704
39	24.658053918397542	25.26530621887305	26.50634833201499	23.570291530714414
40	24.833677914135794	25.97012521847184	26.049540889227146	23.146655978165217
41	26.030326785445002	25.3419657681842	25.742705826165118	22.885001620205678
42	26.0140853555062	25.305624338284773	25.25841591526265	23.421874390946375
43	25.44722992076938	24.436166455560887	25.743512976016625	24.373090647653104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2312.0
1	1843.0
2	1374.0
3	1720.5
4	2067.0
5	2067.0
6	2989.0
7	3911.0
8	3164.5
9	2418.0
10	3580.0
11	4742.0
12	4742.0
13	8808.0
14	12874.0
15	26473.5
16	40073.0
17	37391.0
18	34709.0
19	34709.0
20	37208.5
21	39708.0
22	34492.0
23	29276.0
24	34527.0
25	39778.0
26	39778.0
27	46894.0
28	54010.0
29	64369.5
30	74729.0
31	87625.0
32	100521.0
33	100521.0
34	114983.0
35	129445.0
36	146901.0
37	164357.0
38	191211.5
39	218066.0
40	218066.0
41	244336.5
42	270607.0
43	299829.0
44	329051.0
45	376016.5
46	422982.0
47	422982.0
48	466624.5
49	510267.0
50	518289.0
51	526311.0
52	527330.5
53	528350.0
54	528350.0
55	505261.0
56	482172.0
57	413622.5
58	345073.0
59	290140.0
60	235207.0
61	235207.0
62	199953.5
63	164700.0
64	147786.0
65	130872.0
66	102379.0
67	73886.0
68	73886.0
69	58247.5
70	42609.0
71	35461.0
72	28313.0
73	21826.5
74	15340.0
75	15340.0
76	12142.5
77	8945.0
78	6934.0
79	4923.0
80	3787.0
81	2651.0
82	2651.0
83	2132.0
84	1613.0
85	1213.5
86	814.0
87	600.0
88	386.0
89	386.0
90	251.0
91	116.0
92	73.0
93	30.0
94	19.5
95	9.0
96	9.0
97	6.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5079602.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.573778547973156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.73206328552166	34.648117749658766
2	12.089251807470621	10.777272657537592
3	3.9658777185022602	5.303224655185821
4	1.8912579183493914	3.3720204651842582
5	1.0711463035671929	2.3872519063842037
6	0.6913960037381772	1.8490879415747479
7	0.4769003787209255	1.4880076309385744
8	0.3402676352127296	1.213361137521178
9	0.249137456840244	0.999449804628203
>10	1.3348960540288324	10.541305533575294
>50	0.07666920099089894	2.3139772040979754
>100	0.054051042584952795	5.221939373059794
>500	0.01349834102646316	4.2505269897188285
>1k	0.012568945858526852	11.448536030821064
>5k	7.966233290615371E-4	2.3242096435173374
>10k+	2.2128425807264917E-4	1.8617112765963486
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26022	0.5122842301424403	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21649	0.42619480817591615	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21491	0.42308432826036374	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	13554	0.26683192895821367	No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC	11658	0.2295061699715844	No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC	9502	0.1870618997315144	No Hit
GGTCTAGGACATGCGACTGAATCGCCCTATTCAGACTCGCTTT	7546	0.14855494584024495	No Hit
ATAGTGAACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGG	7205	0.14184182146554	No Hit
AGGCTAAACCGATCATGTGTGATAGCCGGCAGGCGTTGCATGG	7129	0.1403456412529958	No Hit
ATCCACAGCTCATCCCCTCCATTTTTAACTGAAGTGGGTTCGG	7023	0.13825886358813153	No Hit
AGTCTGAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGC	6937	0.13656581755814728	No Hit
TATCAACGCAGAGTACGGGAATATAAACCCGTTGTCCATCGAC	6905	0.13593584694233918	No Hit
GGTAGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAG	6486	0.127687169191602	No Hit
GCTGTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCT	6333	0.1246751221847696	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6295	0.12392703207849749	No Hit
TCCTAGACCCGAAGCGAAGTGATCTATCCATGGCCAGGTTGAA	6286	0.12374985284280147	No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGT	6262	0.12327737488094541	No Hit
TTACAACGTTGTATAGACGAACAGGATTGAAAGCCTGGTCATA	6197	0.12199774706758522	No Hit
GGATAGGGGTGAAAGGCCAATCAAACTTCGTGATAGCTGGTTC	5933	0.11680048948716848	No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCG	5816	0.11449715942312017	No Hit
ATGTAACGGGGCTCAAGCACGCCACCGAAGTTGTGGCATTGAT	5659	0.11140636608931172	No Hit
GAATAGGGCGATTCAGTCGCATGTCCTAGACCCGAAGCGAAGT	5200	0.10237022506881445	No Hit
GGTTTACACCGCCGCTTCGCTGGCCACACGACGCTCTCCTACC	5105	0.10049999980313418	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9686581744002777E-5	0.0	0.0	0.0	0.0
2	1.9686581744002777E-5	0.0	0.0	1.9686581744002777E-5	0.0
3	1.9686581744002777E-5	0.0	0.0	3.9373163488005554E-5	0.0
4	1.9686581744002777E-5	0.0	0.0	1.3780607220801946E-4	0.0
5	3.9373163488005554E-5	0.0	0.0	1.5749265395202222E-4	0.0
6	7.874632697601111E-5	0.0	0.0	1.5749265395202222E-4	0.0
7	7.874632697601111E-5	1.9686581744002777E-5	0.0	1.77179235696025E-4	0.0
8	7.874632697601111E-5	1.9686581744002777E-5	0.0	1.77179235696025E-4	0.0
9	7.874632697601111E-5	1.9686581744002777E-5	0.0	1.9686581744002778E-4	1.9686581744002777E-5
10	7.874632697601111E-5	1.9686581744002777E-5	0.0	2.3623898092803335E-4	3.9373163488005554E-5
11	7.874632697601111E-5	1.9686581744002777E-5	0.0	2.756121444160389E-4	3.9373163488005554E-5
12	7.874632697601111E-5	1.9686581744002777E-5	0.0	2.756121444160389E-4	3.9373163488005554E-5
13	7.874632697601111E-5	1.9686581744002777E-5	0.0	2.756121444160389E-4	5.905974523200834E-5
14	9.843290872001389E-5	1.9686581744002777E-5	0.0	2.9529872616004165E-4	7.874632697601111E-5
15	1.1811949046401668E-4	1.9686581744002777E-5	0.0	2.9529872616004165E-4	7.874632697601111E-5
16	1.1811949046401668E-4	1.9686581744002777E-5	0.0	3.1498530790404443E-4	9.843290872001389E-5
17	1.1811949046401668E-4	1.9686581744002777E-5	0.0	3.1498530790404443E-4	9.843290872001389E-5
18	1.1811949046401668E-4	1.9686581744002777E-5	1.9686581744002777E-5	3.1498530790404443E-4	9.843290872001389E-5
19	1.1811949046401668E-4	1.9686581744002777E-5	1.9686581744002777E-5	3.5435847139205E-4	9.843290872001389E-5
20	1.3780607220801946E-4	1.9686581744002777E-5	1.9686581744002777E-5	4.1341821662405835E-4	9.843290872001389E-5
21	1.77179235696025E-4	1.9686581744002777E-5	1.9686581744002777E-5	5.118511253440723E-4	9.843290872001389E-5
22	1.77179235696025E-4	1.9686581744002777E-5	1.9686581744002777E-5	5.905974523200833E-4	9.843290872001389E-5
23	1.9686581744002778E-4	1.9686581744002777E-5	1.9686581744002777E-5	6.102840340640861E-4	1.1811949046401668E-4
24	2.1655239918403057E-4	1.9686581744002777E-5	1.9686581744002777E-5	8.662095967361223E-4	1.1811949046401668E-4
25	2.1655239918403057E-4	1.9686581744002777E-5	1.9686581744002777E-5	9.449559237121334E-4	1.1811949046401668E-4
26	2.5592556267203613E-4	1.9686581744002777E-5	1.9686581744002777E-5	0.0010433888324321472	1.1811949046401668E-4
27	2.5592556267203613E-4	1.9686581744002777E-5	1.9686581744002777E-5	0.0013780607220801944	1.1811949046401668E-4
28	2.5592556267203613E-4	1.9686581744002777E-5	1.9686581744002777E-5	0.002756121444160389	1.3780607220801946E-4
29	2.5592556267203613E-4	1.9686581744002777E-5	1.9686581744002777E-5	0.005472869724832772	1.5749265395202222E-4
30	2.5592556267203613E-4	1.9686581744002777E-5	1.9686581744002777E-5	0.009055827602241278	1.5749265395202222E-4
31	2.5592556267203613E-4	1.9686581744002777E-5	1.9686581744002777E-5	0.014134965692193994	1.5749265395202222E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6760	0.0	30.81509	1
TACGCAT	2095	0.0	21.811457	19
CTACGCA	2155	0.0	21.204178	18
CGCTACG	2200	0.0	20.770454	16
ACGCATT	2290	0.0	20.115719	20
CGCATTC	2285	0.0	20.078773	21
GCTACGC	2285	0.0	19.99781	17
GCTTTCG	2480	0.0	18.649193	11
CGTATCG	90	2.155166E-6	18.5	10
CGGGTTA	2525	0.0	18.390099	34
TCGGGTT	2575	0.0	18.033009	33
CGCTTTC	2575	0.0	18.033009	10
ATTCCCC	2710	0.0	17.271217	24
CTCGGGT	2775	0.0	16.866667	32
CTCTCGG	2815	0.0	16.692719	30
GTATCAA	12480	0.0	16.617388	2
TTCGCTA	2805	0.0	16.488413	14
TCGCTAC	2880	0.0	16.123264	15
GCATTCC	2910	0.0	15.893471	22
CCCCTCT	2965	0.0	15.848229	27
>>END_MODULE